Expression of Cucumber mosaic virus suppressor 2b alters FWA methylation and its siRNA accumulation in Arabidopsis thaliana

The Cucumber mosaic virus (CMV) suppressor 2b co-localizes with AGO4 in cytoplasmic and nuclear fractions of Arabidopsis thaliana. Biochemical fractionation of A. thaliana cellular extracts revealed that 2b and AGO4 coexist in multiple size exclusions. 2b transgenic A. thaliana exhibited an enhanced...

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Autores principales: Hamera, Sadia, Yan, Youngsheng, Song, Xiaoguang, Chaudhary, Safee Ullah, Murtaza, Iram, Su, Lei, Tariq, Muhammad, Chen, Xiaoying, Fang, Rongxiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Ltd 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5155521/
https://www.ncbi.nlm.nih.gov/pubmed/27659688
http://dx.doi.org/10.1242/bio.017244
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author Hamera, Sadia
Yan, Youngsheng
Song, Xiaoguang
Chaudhary, Safee Ullah
Murtaza, Iram
Su, Lei
Tariq, Muhammad
Chen, Xiaoying
Fang, Rongxiang
author_facet Hamera, Sadia
Yan, Youngsheng
Song, Xiaoguang
Chaudhary, Safee Ullah
Murtaza, Iram
Su, Lei
Tariq, Muhammad
Chen, Xiaoying
Fang, Rongxiang
author_sort Hamera, Sadia
collection PubMed
description The Cucumber mosaic virus (CMV) suppressor 2b co-localizes with AGO4 in cytoplasmic and nuclear fractions of Arabidopsis thaliana. Biochemical fractionation of A. thaliana cellular extracts revealed that 2b and AGO4 coexist in multiple size exclusions. 2b transgenic A. thaliana exhibited an enhanced accumulation of 24nt siRNAs from flowering wageningen (FWA) and other heterochromatic loci. These plants also exhibited hypo-methylation of an endogenous- as well as transgene-FWA promoter at non-CG sites. In corroboration, both transgenic 2b and CMV infection affected the regulation of transposons which mimics the ago4 phenotype. In conclusion, 2b perturbs plant defense by interfering with AGO4-regulated transcriptional gene silencing.
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spelling pubmed-51555212016-12-16 Expression of Cucumber mosaic virus suppressor 2b alters FWA methylation and its siRNA accumulation in Arabidopsis thaliana Hamera, Sadia Yan, Youngsheng Song, Xiaoguang Chaudhary, Safee Ullah Murtaza, Iram Su, Lei Tariq, Muhammad Chen, Xiaoying Fang, Rongxiang Biol Open Research Article The Cucumber mosaic virus (CMV) suppressor 2b co-localizes with AGO4 in cytoplasmic and nuclear fractions of Arabidopsis thaliana. Biochemical fractionation of A. thaliana cellular extracts revealed that 2b and AGO4 coexist in multiple size exclusions. 2b transgenic A. thaliana exhibited an enhanced accumulation of 24nt siRNAs from flowering wageningen (FWA) and other heterochromatic loci. These plants also exhibited hypo-methylation of an endogenous- as well as transgene-FWA promoter at non-CG sites. In corroboration, both transgenic 2b and CMV infection affected the regulation of transposons which mimics the ago4 phenotype. In conclusion, 2b perturbs plant defense by interfering with AGO4-regulated transcriptional gene silencing. The Company of Biologists Ltd 2016-09-22 /pmc/articles/PMC5155521/ /pubmed/27659688 http://dx.doi.org/10.1242/bio.017244 Text en © 2016. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
spellingShingle Research Article
Hamera, Sadia
Yan, Youngsheng
Song, Xiaoguang
Chaudhary, Safee Ullah
Murtaza, Iram
Su, Lei
Tariq, Muhammad
Chen, Xiaoying
Fang, Rongxiang
Expression of Cucumber mosaic virus suppressor 2b alters FWA methylation and its siRNA accumulation in Arabidopsis thaliana
title Expression of Cucumber mosaic virus suppressor 2b alters FWA methylation and its siRNA accumulation in Arabidopsis thaliana
title_full Expression of Cucumber mosaic virus suppressor 2b alters FWA methylation and its siRNA accumulation in Arabidopsis thaliana
title_fullStr Expression of Cucumber mosaic virus suppressor 2b alters FWA methylation and its siRNA accumulation in Arabidopsis thaliana
title_full_unstemmed Expression of Cucumber mosaic virus suppressor 2b alters FWA methylation and its siRNA accumulation in Arabidopsis thaliana
title_short Expression of Cucumber mosaic virus suppressor 2b alters FWA methylation and its siRNA accumulation in Arabidopsis thaliana
title_sort expression of cucumber mosaic virus suppressor 2b alters fwa methylation and its sirna accumulation in arabidopsis thaliana
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5155521/
https://www.ncbi.nlm.nih.gov/pubmed/27659688
http://dx.doi.org/10.1242/bio.017244
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