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Ecdysone signaling induces two phases of cell cycle exit in Drosophila cells
During development, cell proliferation and differentiation must be tightly coordinated to ensure proper tissue morphogenesis. Because steroid hormones are central regulators of developmental timing, understanding the links between steroid hormone signaling and cell proliferation is crucial to unders...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Company of Biologists Ltd
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5155522/ https://www.ncbi.nlm.nih.gov/pubmed/27737823 http://dx.doi.org/10.1242/bio.017525 |
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author | Guo, Yongfeng Flegel, Kerry Kumar, Jayashree McKay, Daniel J. Buttitta, Laura A. |
author_facet | Guo, Yongfeng Flegel, Kerry Kumar, Jayashree McKay, Daniel J. Buttitta, Laura A. |
author_sort | Guo, Yongfeng |
collection | PubMed |
description | During development, cell proliferation and differentiation must be tightly coordinated to ensure proper tissue morphogenesis. Because steroid hormones are central regulators of developmental timing, understanding the links between steroid hormone signaling and cell proliferation is crucial to understanding the molecular basis of morphogenesis. Here we examined the mechanism by which the steroid hormone ecdysone regulates the cell cycle in Drosophila. We find that a cell cycle arrest induced by ecdysone in Drosophila cell culture is analogous to a G2 cell cycle arrest observed in the early pupa wing. We show that in the wing, ecdysone signaling at the larva-to-puparium transition induces Broad which in turn represses the cdc25c phosphatase String. The repression of String generates a temporary G2 arrest that synchronizes the cell cycle in the wing epithelium during early pupa wing elongation and flattening. As ecdysone levels decline after the larva-to-puparium pulse during early metamorphosis, Broad expression plummets, allowing String to become re-activated, which promotes rapid G2/M progression and a subsequent synchronized final cell cycle in the wing. In this manner, pulses of ecdysone can both synchronize the final cell cycle and promote the coordinated acquisition of terminal differentiation characteristics in the wing. |
format | Online Article Text |
id | pubmed-5155522 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | The Company of Biologists Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-51555222016-12-16 Ecdysone signaling induces two phases of cell cycle exit in Drosophila cells Guo, Yongfeng Flegel, Kerry Kumar, Jayashree McKay, Daniel J. Buttitta, Laura A. Biol Open Research Article During development, cell proliferation and differentiation must be tightly coordinated to ensure proper tissue morphogenesis. Because steroid hormones are central regulators of developmental timing, understanding the links between steroid hormone signaling and cell proliferation is crucial to understanding the molecular basis of morphogenesis. Here we examined the mechanism by which the steroid hormone ecdysone regulates the cell cycle in Drosophila. We find that a cell cycle arrest induced by ecdysone in Drosophila cell culture is analogous to a G2 cell cycle arrest observed in the early pupa wing. We show that in the wing, ecdysone signaling at the larva-to-puparium transition induces Broad which in turn represses the cdc25c phosphatase String. The repression of String generates a temporary G2 arrest that synchronizes the cell cycle in the wing epithelium during early pupa wing elongation and flattening. As ecdysone levels decline after the larva-to-puparium pulse during early metamorphosis, Broad expression plummets, allowing String to become re-activated, which promotes rapid G2/M progression and a subsequent synchronized final cell cycle in the wing. In this manner, pulses of ecdysone can both synchronize the final cell cycle and promote the coordinated acquisition of terminal differentiation characteristics in the wing. The Company of Biologists Ltd 2016-10-13 /pmc/articles/PMC5155522/ /pubmed/27737823 http://dx.doi.org/10.1242/bio.017525 Text en © 2016. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
spellingShingle | Research Article Guo, Yongfeng Flegel, Kerry Kumar, Jayashree McKay, Daniel J. Buttitta, Laura A. Ecdysone signaling induces two phases of cell cycle exit in Drosophila cells |
title | Ecdysone signaling induces two phases of cell cycle exit in Drosophila cells |
title_full | Ecdysone signaling induces two phases of cell cycle exit in Drosophila cells |
title_fullStr | Ecdysone signaling induces two phases of cell cycle exit in Drosophila cells |
title_full_unstemmed | Ecdysone signaling induces two phases of cell cycle exit in Drosophila cells |
title_short | Ecdysone signaling induces two phases of cell cycle exit in Drosophila cells |
title_sort | ecdysone signaling induces two phases of cell cycle exit in drosophila cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5155522/ https://www.ncbi.nlm.nih.gov/pubmed/27737823 http://dx.doi.org/10.1242/bio.017525 |
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