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In vitro fermentation of B-GOS: impact on faecal bacterial populations and metabolic activity in autistic and non-autistic children

Children with autism spectrum disorders (ASD) often suffer gastrointestinal problems consistent with imbalances in the gut microbial population. Treatment with antibiotics or pro/prebiotics has been postulated to regulate microbiota and improve gut symptoms, but there is a lack of evidence for such...

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Autores principales: Grimaldi, Roberta, Cela, Drinalda, Swann, Jonathan R., Vulevic, Jelena, Gibson, Glenn R., Tzortzis, George, Costabile, Adele
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5155555/
https://www.ncbi.nlm.nih.gov/pubmed/27856622
http://dx.doi.org/10.1093/femsec/fiw233
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author Grimaldi, Roberta
Cela, Drinalda
Swann, Jonathan R.
Vulevic, Jelena
Gibson, Glenn R.
Tzortzis, George
Costabile, Adele
author_facet Grimaldi, Roberta
Cela, Drinalda
Swann, Jonathan R.
Vulevic, Jelena
Gibson, Glenn R.
Tzortzis, George
Costabile, Adele
author_sort Grimaldi, Roberta
collection PubMed
description Children with autism spectrum disorders (ASD) often suffer gastrointestinal problems consistent with imbalances in the gut microbial population. Treatment with antibiotics or pro/prebiotics has been postulated to regulate microbiota and improve gut symptoms, but there is a lack of evidence for such approaches, especially for prebiotics. This study assessed the influence of a prebiotic galactooligosaccharide (B-GOS) on gut microbial ecology and metabolic function using faecal samples from autistic and non-autistic children in an in vitro gut model system. Bacteriology was analysed using flow cytometry combined with fluorescence in situ hybridization and metabolic activity by HPLC and (1)H-NMR. Consistent with previous studies, the microbiota of children with ASD contained a higher number of Clostridium spp. and a lower number of bifidobacteria compared with non-autistic children. B-GOS administration significantly increased bifidobacterial populations in each compartment of the models, both with autistic and non-autistic-derived samples, and lactobacilli in the final vessel of non-autistic models. In addition, changes in other bacterial population have been seen in particular for Clostridium, Rosburia, Bacteroides, Atopobium, Faecalibacterium prausnitzii, Sutterella spp. and Veillonellaceae. Furthermore, the addition of B-GOS to the models significantly altered short-chain fatty acid production in both groups, and increased ethanol and lactate in autistic children.
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spelling pubmed-51555552016-12-16 In vitro fermentation of B-GOS: impact on faecal bacterial populations and metabolic activity in autistic and non-autistic children Grimaldi, Roberta Cela, Drinalda Swann, Jonathan R. Vulevic, Jelena Gibson, Glenn R. Tzortzis, George Costabile, Adele FEMS Microbiol Ecol Research Article Children with autism spectrum disorders (ASD) often suffer gastrointestinal problems consistent with imbalances in the gut microbial population. Treatment with antibiotics or pro/prebiotics has been postulated to regulate microbiota and improve gut symptoms, but there is a lack of evidence for such approaches, especially for prebiotics. This study assessed the influence of a prebiotic galactooligosaccharide (B-GOS) on gut microbial ecology and metabolic function using faecal samples from autistic and non-autistic children in an in vitro gut model system. Bacteriology was analysed using flow cytometry combined with fluorescence in situ hybridization and metabolic activity by HPLC and (1)H-NMR. Consistent with previous studies, the microbiota of children with ASD contained a higher number of Clostridium spp. and a lower number of bifidobacteria compared with non-autistic children. B-GOS administration significantly increased bifidobacterial populations in each compartment of the models, both with autistic and non-autistic-derived samples, and lactobacilli in the final vessel of non-autistic models. In addition, changes in other bacterial population have been seen in particular for Clostridium, Rosburia, Bacteroides, Atopobium, Faecalibacterium prausnitzii, Sutterella spp. and Veillonellaceae. Furthermore, the addition of B-GOS to the models significantly altered short-chain fatty acid production in both groups, and increased ethanol and lactate in autistic children. Oxford University Press 2016-11-16 2017-02 /pmc/articles/PMC5155555/ /pubmed/27856622 http://dx.doi.org/10.1093/femsec/fiw233 Text en © FEMS 2016. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Research Article
Grimaldi, Roberta
Cela, Drinalda
Swann, Jonathan R.
Vulevic, Jelena
Gibson, Glenn R.
Tzortzis, George
Costabile, Adele
In vitro fermentation of B-GOS: impact on faecal bacterial populations and metabolic activity in autistic and non-autistic children
title In vitro fermentation of B-GOS: impact on faecal bacterial populations and metabolic activity in autistic and non-autistic children
title_full In vitro fermentation of B-GOS: impact on faecal bacterial populations and metabolic activity in autistic and non-autistic children
title_fullStr In vitro fermentation of B-GOS: impact on faecal bacterial populations and metabolic activity in autistic and non-autistic children
title_full_unstemmed In vitro fermentation of B-GOS: impact on faecal bacterial populations and metabolic activity in autistic and non-autistic children
title_short In vitro fermentation of B-GOS: impact on faecal bacterial populations and metabolic activity in autistic and non-autistic children
title_sort in vitro fermentation of b-gos: impact on faecal bacterial populations and metabolic activity in autistic and non-autistic children
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5155555/
https://www.ncbi.nlm.nih.gov/pubmed/27856622
http://dx.doi.org/10.1093/femsec/fiw233
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