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Cubozoan genome illuminates functional diversification of opsins and photoreceptor evolution

Animals sense light primarily by an opsin-based photopigment present in a photoreceptor cell. Cnidaria are arguably the most basal phylum containing a well-developed visual system. The evolutionary history of opsins in the animal kingdom has not yet been resolved. Here, we study the evolution of ani...

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Autores principales: Liegertová, Michaela, Pergner, Jiří, Kozmiková, Iryna, Fabian, Peter, Pombinho, Antonio R., Strnad, Hynek, Pačes, Jan, Vlček, Čestmír, Bartůněk, Petr, Kozmik, Zbyněk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5155618/
https://www.ncbi.nlm.nih.gov/pubmed/26154478
http://dx.doi.org/10.1038/srep11885
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author Liegertová, Michaela
Pergner, Jiří
Kozmiková, Iryna
Fabian, Peter
Pombinho, Antonio R.
Strnad, Hynek
Pačes, Jan
Vlček, Čestmír
Bartůněk, Petr
Kozmik, Zbyněk
author_facet Liegertová, Michaela
Pergner, Jiří
Kozmiková, Iryna
Fabian, Peter
Pombinho, Antonio R.
Strnad, Hynek
Pačes, Jan
Vlček, Čestmír
Bartůněk, Petr
Kozmik, Zbyněk
author_sort Liegertová, Michaela
collection PubMed
description Animals sense light primarily by an opsin-based photopigment present in a photoreceptor cell. Cnidaria are arguably the most basal phylum containing a well-developed visual system. The evolutionary history of opsins in the animal kingdom has not yet been resolved. Here, we study the evolution of animal opsins by genome-wide analysis of the cubozoan jellyfish Tripedalia cystophora, a cnidarian possessing complex lens-containing eyes and minor photoreceptors. A large number of opsin genes with distinct tissue- and stage-specific expression were identified. Our phylogenetic analysis unequivocally classifies cubozoan opsins as a sister group to c-opsins and documents lineage-specific expansion of the opsin gene repertoire in the cubozoan genome. Functional analyses provided evidence for the use of the Gs-cAMP signaling pathway in a small set of cubozoan opsins, indicating the possibility that the majority of other cubozoan opsins signal via distinct pathways. Additionally, these tests uncovered subtle differences among individual opsins, suggesting possible fine-tuning for specific photoreceptor tasks. Based on phylogenetic, expression and biochemical analysis we propose that rapid lineage- and species-specific duplications of the intron-less opsin genes and their subsequent functional diversification promoted evolution of a large repertoire of both visual and extraocular photoreceptors in cubozoans.
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spelling pubmed-51556182016-12-20 Cubozoan genome illuminates functional diversification of opsins and photoreceptor evolution Liegertová, Michaela Pergner, Jiří Kozmiková, Iryna Fabian, Peter Pombinho, Antonio R. Strnad, Hynek Pačes, Jan Vlček, Čestmír Bartůněk, Petr Kozmik, Zbyněk Sci Rep Article Animals sense light primarily by an opsin-based photopigment present in a photoreceptor cell. Cnidaria are arguably the most basal phylum containing a well-developed visual system. The evolutionary history of opsins in the animal kingdom has not yet been resolved. Here, we study the evolution of animal opsins by genome-wide analysis of the cubozoan jellyfish Tripedalia cystophora, a cnidarian possessing complex lens-containing eyes and minor photoreceptors. A large number of opsin genes with distinct tissue- and stage-specific expression were identified. Our phylogenetic analysis unequivocally classifies cubozoan opsins as a sister group to c-opsins and documents lineage-specific expansion of the opsin gene repertoire in the cubozoan genome. Functional analyses provided evidence for the use of the Gs-cAMP signaling pathway in a small set of cubozoan opsins, indicating the possibility that the majority of other cubozoan opsins signal via distinct pathways. Additionally, these tests uncovered subtle differences among individual opsins, suggesting possible fine-tuning for specific photoreceptor tasks. Based on phylogenetic, expression and biochemical analysis we propose that rapid lineage- and species-specific duplications of the intron-less opsin genes and their subsequent functional diversification promoted evolution of a large repertoire of both visual and extraocular photoreceptors in cubozoans. Nature Publishing Group 2015-07-08 /pmc/articles/PMC5155618/ /pubmed/26154478 http://dx.doi.org/10.1038/srep11885 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Liegertová, Michaela
Pergner, Jiří
Kozmiková, Iryna
Fabian, Peter
Pombinho, Antonio R.
Strnad, Hynek
Pačes, Jan
Vlček, Čestmír
Bartůněk, Petr
Kozmik, Zbyněk
Cubozoan genome illuminates functional diversification of opsins and photoreceptor evolution
title Cubozoan genome illuminates functional diversification of opsins and photoreceptor evolution
title_full Cubozoan genome illuminates functional diversification of opsins and photoreceptor evolution
title_fullStr Cubozoan genome illuminates functional diversification of opsins and photoreceptor evolution
title_full_unstemmed Cubozoan genome illuminates functional diversification of opsins and photoreceptor evolution
title_short Cubozoan genome illuminates functional diversification of opsins and photoreceptor evolution
title_sort cubozoan genome illuminates functional diversification of opsins and photoreceptor evolution
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5155618/
https://www.ncbi.nlm.nih.gov/pubmed/26154478
http://dx.doi.org/10.1038/srep11885
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