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Molecular analysis of idiopathic subglottic stenosis for Mycobacterium species

OBJECTIVES/HYPOTHESIS: Idiopathic subglottic stenosis (iSGS) is an unexplained obstruction involving the lower laryngeal and upper tracheal airway. Persistent mucosal inflammation is a hallmark of the disease. Epithelial microbiota dysbiosis is found in other chronic inflammatory mucosal diseases; h...

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Autores principales: Gelbard, Alexander, Katsantonis, Nicolas‐George, Mizuta, Masanobu, Newcomb, Dawn, Rotsinger, Joseph, Rousseau, Bernard, Daniero, James J., Edell, Eric S., Ekbom, Dale C., Kasperbauer, Jan L., Hillel, Alexander T., Yang, Liying, Garrett, C. Gaelyn, Netterville, James L., Wootten, Christopher T., Francis, David O., Stratton, Charles, Jenkins, Kevin, McGregor, Tracy L., Gaddy, Jennifer A., Blackwell, Timothy S., Drake, Wonder P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5156582/
https://www.ncbi.nlm.nih.gov/pubmed/27295947
http://dx.doi.org/10.1002/lary.26097
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author Gelbard, Alexander
Katsantonis, Nicolas‐George
Mizuta, Masanobu
Newcomb, Dawn
Rotsinger, Joseph
Rousseau, Bernard
Daniero, James J.
Edell, Eric S.
Ekbom, Dale C.
Kasperbauer, Jan L.
Hillel, Alexander T.
Yang, Liying
Garrett, C. Gaelyn
Netterville, James L.
Wootten, Christopher T.
Francis, David O.
Stratton, Charles
Jenkins, Kevin
McGregor, Tracy L.
Gaddy, Jennifer A.
Blackwell, Timothy S.
Drake, Wonder P.
author_facet Gelbard, Alexander
Katsantonis, Nicolas‐George
Mizuta, Masanobu
Newcomb, Dawn
Rotsinger, Joseph
Rousseau, Bernard
Daniero, James J.
Edell, Eric S.
Ekbom, Dale C.
Kasperbauer, Jan L.
Hillel, Alexander T.
Yang, Liying
Garrett, C. Gaelyn
Netterville, James L.
Wootten, Christopher T.
Francis, David O.
Stratton, Charles
Jenkins, Kevin
McGregor, Tracy L.
Gaddy, Jennifer A.
Blackwell, Timothy S.
Drake, Wonder P.
author_sort Gelbard, Alexander
collection PubMed
description OBJECTIVES/HYPOTHESIS: Idiopathic subglottic stenosis (iSGS) is an unexplained obstruction involving the lower laryngeal and upper tracheal airway. Persistent mucosal inflammation is a hallmark of the disease. Epithelial microbiota dysbiosis is found in other chronic inflammatory mucosal diseases; however, the relationship between tracheal microbiota composition and iSGS is unknown. Given the critical role for host defense at mucosal barriers, we analyzed tissue specimens from iSGS patients for the presence of microbial pathogens. METHODS: Utilizing 30 human iSGS, 20 intubation‐related tracheal stenosis (iLTS), and 20 healthy control specimens, we applied molecular, immunohistochemical, electron microscopic, immunologic, and Sanger‐sequencing techniques. RESULTS: With unbiased culture‐independent nucleic acid, protein, and immunologic approaches, we demonstrate that Mycobacterium species are uniquely associated with iSGS. Phylogenetic analysis of the mycobacterial virulence factor rpoB suggests that, rather than Mycobacterium tuberculosis, a variant member of the Mycobacterium tuberculosis complex or a closely related novel mycobacterium is present in iSGS specimens. CONCLUSION: These studies identify a novel pathogenic role for established large airway bacteria and provide new targets for future therapeutic intervention. LEVEL OF EVIDENCE: NA Laryngoscope, 127:179–185, 2017
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spelling pubmed-51565822018-01-01 Molecular analysis of idiopathic subglottic stenosis for Mycobacterium species Gelbard, Alexander Katsantonis, Nicolas‐George Mizuta, Masanobu Newcomb, Dawn Rotsinger, Joseph Rousseau, Bernard Daniero, James J. Edell, Eric S. Ekbom, Dale C. Kasperbauer, Jan L. Hillel, Alexander T. Yang, Liying Garrett, C. Gaelyn Netterville, James L. Wootten, Christopher T. Francis, David O. Stratton, Charles Jenkins, Kevin McGregor, Tracy L. Gaddy, Jennifer A. Blackwell, Timothy S. Drake, Wonder P. Laryngoscope Laryngology OBJECTIVES/HYPOTHESIS: Idiopathic subglottic stenosis (iSGS) is an unexplained obstruction involving the lower laryngeal and upper tracheal airway. Persistent mucosal inflammation is a hallmark of the disease. Epithelial microbiota dysbiosis is found in other chronic inflammatory mucosal diseases; however, the relationship between tracheal microbiota composition and iSGS is unknown. Given the critical role for host defense at mucosal barriers, we analyzed tissue specimens from iSGS patients for the presence of microbial pathogens. METHODS: Utilizing 30 human iSGS, 20 intubation‐related tracheal stenosis (iLTS), and 20 healthy control specimens, we applied molecular, immunohistochemical, electron microscopic, immunologic, and Sanger‐sequencing techniques. RESULTS: With unbiased culture‐independent nucleic acid, protein, and immunologic approaches, we demonstrate that Mycobacterium species are uniquely associated with iSGS. Phylogenetic analysis of the mycobacterial virulence factor rpoB suggests that, rather than Mycobacterium tuberculosis, a variant member of the Mycobacterium tuberculosis complex or a closely related novel mycobacterium is present in iSGS specimens. CONCLUSION: These studies identify a novel pathogenic role for established large airway bacteria and provide new targets for future therapeutic intervention. LEVEL OF EVIDENCE: NA Laryngoscope, 127:179–185, 2017 John Wiley and Sons Inc. 2016-06-14 2017-01 /pmc/articles/PMC5156582/ /pubmed/27295947 http://dx.doi.org/10.1002/lary.26097 Text en © 2016 The American Laryngological, Rhinological and Otological Society, Inc. This article is being made freely available through PubMed Central as part of the COVID-19 public health emergency response. It can be used for unrestricted research re-use and analysis in any form or by any means with acknowledgement of the original source, for the duration of the public health emergency.
spellingShingle Laryngology
Gelbard, Alexander
Katsantonis, Nicolas‐George
Mizuta, Masanobu
Newcomb, Dawn
Rotsinger, Joseph
Rousseau, Bernard
Daniero, James J.
Edell, Eric S.
Ekbom, Dale C.
Kasperbauer, Jan L.
Hillel, Alexander T.
Yang, Liying
Garrett, C. Gaelyn
Netterville, James L.
Wootten, Christopher T.
Francis, David O.
Stratton, Charles
Jenkins, Kevin
McGregor, Tracy L.
Gaddy, Jennifer A.
Blackwell, Timothy S.
Drake, Wonder P.
Molecular analysis of idiopathic subglottic stenosis for Mycobacterium species
title Molecular analysis of idiopathic subglottic stenosis for Mycobacterium species
title_full Molecular analysis of idiopathic subglottic stenosis for Mycobacterium species
title_fullStr Molecular analysis of idiopathic subglottic stenosis for Mycobacterium species
title_full_unstemmed Molecular analysis of idiopathic subglottic stenosis for Mycobacterium species
title_short Molecular analysis of idiopathic subglottic stenosis for Mycobacterium species
title_sort molecular analysis of idiopathic subglottic stenosis for mycobacterium species
topic Laryngology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5156582/
https://www.ncbi.nlm.nih.gov/pubmed/27295947
http://dx.doi.org/10.1002/lary.26097
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