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Capillary isoelectric focusing after sample enrichment with immunoaffinity chromatography in a single capillary
For accurate micro-scale quantification of a specific protein in biological fluids, immunoaffinity chromatography (IAC) and isoelectric focusing (IEF) were combined in a single fused-silica capillary. The inner wall of the capillary was coated with an anti-E-tag antibody at the inlet side to form an...
Autores principales: | Shimura, Kiyohito, Nagai, Toshihiko |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5156949/ https://www.ncbi.nlm.nih.gov/pubmed/27976690 http://dx.doi.org/10.1038/srep39221 |
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