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The proteomic profile of whole and glandular saliva in healthy pain-free subjects
Determination of the variability in the salivary proteome is a prerequisite for the development of saliva as a diagnostic and prognostic tool in particular physiological states. In this context, it is important that technical variability induced by sample collection and processing is kept at minimum...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5157045/ https://www.ncbi.nlm.nih.gov/pubmed/27976689 http://dx.doi.org/10.1038/srep39073 |
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author | Jasim, Hajer Olausson, Patrik Hedenberg-Magnusson, Britt Ernberg, Malin Ghafouri, Bijar |
author_facet | Jasim, Hajer Olausson, Patrik Hedenberg-Magnusson, Britt Ernberg, Malin Ghafouri, Bijar |
author_sort | Jasim, Hajer |
collection | PubMed |
description | Determination of the variability in the salivary proteome is a prerequisite for the development of saliva as a diagnostic and prognostic tool in particular physiological states. In this context, it is important that technical variability induced by sample collection and processing is kept at minimum to be able to reproducibly assess variability in states of health and disease. In the current study, the proteome profile in unstimulated and stimulated whole, parotid and sublingual saliva was investigated using two-dimensional gel electrophoresis. Saliva samples were structurally collected from ten examined and characterized healthy individuals during the exactly same conditions. The results demonstrated that different collection methods provide clear differences in the snapshot of the salivary proteome and also in the relative amount of specific proteins. The variable nature of the salivary proteome suggests that different approaches may have to be adopted when studying its composition or its possible role as an indicator for particular physiological states. The results emphasize the importance of consistency when collecting saliva samples for proteomic analysis. |
format | Online Article Text |
id | pubmed-5157045 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-51570452016-12-20 The proteomic profile of whole and glandular saliva in healthy pain-free subjects Jasim, Hajer Olausson, Patrik Hedenberg-Magnusson, Britt Ernberg, Malin Ghafouri, Bijar Sci Rep Article Determination of the variability in the salivary proteome is a prerequisite for the development of saliva as a diagnostic and prognostic tool in particular physiological states. In this context, it is important that technical variability induced by sample collection and processing is kept at minimum to be able to reproducibly assess variability in states of health and disease. In the current study, the proteome profile in unstimulated and stimulated whole, parotid and sublingual saliva was investigated using two-dimensional gel electrophoresis. Saliva samples were structurally collected from ten examined and characterized healthy individuals during the exactly same conditions. The results demonstrated that different collection methods provide clear differences in the snapshot of the salivary proteome and also in the relative amount of specific proteins. The variable nature of the salivary proteome suggests that different approaches may have to be adopted when studying its composition or its possible role as an indicator for particular physiological states. The results emphasize the importance of consistency when collecting saliva samples for proteomic analysis. Nature Publishing Group 2016-12-15 /pmc/articles/PMC5157045/ /pubmed/27976689 http://dx.doi.org/10.1038/srep39073 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Jasim, Hajer Olausson, Patrik Hedenberg-Magnusson, Britt Ernberg, Malin Ghafouri, Bijar The proteomic profile of whole and glandular saliva in healthy pain-free subjects |
title | The proteomic profile of whole and glandular saliva in healthy pain-free subjects |
title_full | The proteomic profile of whole and glandular saliva in healthy pain-free subjects |
title_fullStr | The proteomic profile of whole and glandular saliva in healthy pain-free subjects |
title_full_unstemmed | The proteomic profile of whole and glandular saliva in healthy pain-free subjects |
title_short | The proteomic profile of whole and glandular saliva in healthy pain-free subjects |
title_sort | proteomic profile of whole and glandular saliva in healthy pain-free subjects |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5157045/ https://www.ncbi.nlm.nih.gov/pubmed/27976689 http://dx.doi.org/10.1038/srep39073 |
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