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The draft genome of whitefly Bemisia tabaci MEAM1, a global crop pest, provides novel insights into virus transmission, host adaptation, and insecticide resistance
BACKGROUND: The whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) is among the 100 worst invasive species in the world. As one of the most important crop pests and virus vectors, B. tabaci causes substantial crop losses and poses a serious threat to global food security. RESULTS: We report the 615-Mb...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5157087/ https://www.ncbi.nlm.nih.gov/pubmed/27974049 http://dx.doi.org/10.1186/s12915-016-0321-y |
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author | Chen, Wenbo Hasegawa, Daniel K. Kaur, Navneet Kliot, Adi Pinheiro, Patricia Valle Luan, Junbo Stensmyr, Marcus C. Zheng, Yi Liu, Wenli Sun, Honghe Xu, Yimin Luo, Yuan Kruse, Angela Yang, Xiaowei Kontsedalov, Svetlana Lebedev, Galina Fisher, Tonja W. Nelson, David R. Hunter, Wayne B. Brown, Judith K. Jander, Georg Cilia, Michelle Douglas, Angela E. Ghanim, Murad Simmons, Alvin M. Wintermantel, William M. Ling, Kai-Shu Fei, Zhangjun |
author_facet | Chen, Wenbo Hasegawa, Daniel K. Kaur, Navneet Kliot, Adi Pinheiro, Patricia Valle Luan, Junbo Stensmyr, Marcus C. Zheng, Yi Liu, Wenli Sun, Honghe Xu, Yimin Luo, Yuan Kruse, Angela Yang, Xiaowei Kontsedalov, Svetlana Lebedev, Galina Fisher, Tonja W. Nelson, David R. Hunter, Wayne B. Brown, Judith K. Jander, Georg Cilia, Michelle Douglas, Angela E. Ghanim, Murad Simmons, Alvin M. Wintermantel, William M. Ling, Kai-Shu Fei, Zhangjun |
author_sort | Chen, Wenbo |
collection | PubMed |
description | BACKGROUND: The whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) is among the 100 worst invasive species in the world. As one of the most important crop pests and virus vectors, B. tabaci causes substantial crop losses and poses a serious threat to global food security. RESULTS: We report the 615-Mb high-quality genome sequence of B. tabaci Middle East-Asia Minor 1 (MEAM1), the first genome sequence in the Aleyrodidae family, which contains 15,664 protein-coding genes. The B. tabaci genome is highly divergent from other sequenced hemipteran genomes, sharing no detectable synteny. A number of known detoxification gene families, including cytochrome P450s and UDP-glucuronosyltransferases, are significantly expanded in B. tabaci. Other expanded gene families, including cathepsins, large clusters of tandemly duplicated B. tabaci-specific genes, and phosphatidylethanolamine-binding proteins (PEBPs), were found to be associated with virus acquisition and transmission and/or insecticide resistance, likely contributing to the global invasiveness and efficient virus transmission capacity of B. tabaci. The presence of 142 horizontally transferred genes from bacteria or fungi in the B. tabaci genome, including genes encoding hopanoid/sterol synthesis and xenobiotic detoxification enzymes that are not present in other insects, offers novel insights into the unique biological adaptations of this insect such as polyphagy and insecticide resistance. Interestingly, two adjacent bacterial pantothenate biosynthesis genes, panB and panC, have been co-transferred into B. tabaci and fused into a single gene that has acquired introns during its evolution. CONCLUSIONS: The B. tabaci genome contains numerous genetic novelties, including expansions in gene families associated with insecticide resistance, detoxification and virus transmission, as well as numerous horizontally transferred genes from bacteria and fungi. We believe these novelties likely have shaped B. tabaci as a highly invasive polyphagous crop pest and efficient vector of plant viruses. The genome serves as a reference for resolving the B. tabaci cryptic species complex, understanding fundamental biological novelties, and providing valuable genetic information to assist the development of novel strategies for controlling whiteflies and the viruses they transmit. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12915-016-0321-y) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5157087 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-51570872016-12-20 The draft genome of whitefly Bemisia tabaci MEAM1, a global crop pest, provides novel insights into virus transmission, host adaptation, and insecticide resistance Chen, Wenbo Hasegawa, Daniel K. Kaur, Navneet Kliot, Adi Pinheiro, Patricia Valle Luan, Junbo Stensmyr, Marcus C. Zheng, Yi Liu, Wenli Sun, Honghe Xu, Yimin Luo, Yuan Kruse, Angela Yang, Xiaowei Kontsedalov, Svetlana Lebedev, Galina Fisher, Tonja W. Nelson, David R. Hunter, Wayne B. Brown, Judith K. Jander, Georg Cilia, Michelle Douglas, Angela E. Ghanim, Murad Simmons, Alvin M. Wintermantel, William M. Ling, Kai-Shu Fei, Zhangjun BMC Biol Research Article BACKGROUND: The whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) is among the 100 worst invasive species in the world. As one of the most important crop pests and virus vectors, B. tabaci causes substantial crop losses and poses a serious threat to global food security. RESULTS: We report the 615-Mb high-quality genome sequence of B. tabaci Middle East-Asia Minor 1 (MEAM1), the first genome sequence in the Aleyrodidae family, which contains 15,664 protein-coding genes. The B. tabaci genome is highly divergent from other sequenced hemipteran genomes, sharing no detectable synteny. A number of known detoxification gene families, including cytochrome P450s and UDP-glucuronosyltransferases, are significantly expanded in B. tabaci. Other expanded gene families, including cathepsins, large clusters of tandemly duplicated B. tabaci-specific genes, and phosphatidylethanolamine-binding proteins (PEBPs), were found to be associated with virus acquisition and transmission and/or insecticide resistance, likely contributing to the global invasiveness and efficient virus transmission capacity of B. tabaci. The presence of 142 horizontally transferred genes from bacteria or fungi in the B. tabaci genome, including genes encoding hopanoid/sterol synthesis and xenobiotic detoxification enzymes that are not present in other insects, offers novel insights into the unique biological adaptations of this insect such as polyphagy and insecticide resistance. Interestingly, two adjacent bacterial pantothenate biosynthesis genes, panB and panC, have been co-transferred into B. tabaci and fused into a single gene that has acquired introns during its evolution. CONCLUSIONS: The B. tabaci genome contains numerous genetic novelties, including expansions in gene families associated with insecticide resistance, detoxification and virus transmission, as well as numerous horizontally transferred genes from bacteria and fungi. We believe these novelties likely have shaped B. tabaci as a highly invasive polyphagous crop pest and efficient vector of plant viruses. The genome serves as a reference for resolving the B. tabaci cryptic species complex, understanding fundamental biological novelties, and providing valuable genetic information to assist the development of novel strategies for controlling whiteflies and the viruses they transmit. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12915-016-0321-y) contains supplementary material, which is available to authorized users. BioMed Central 2016-12-14 /pmc/articles/PMC5157087/ /pubmed/27974049 http://dx.doi.org/10.1186/s12915-016-0321-y Text en © Chen et al. 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Chen, Wenbo Hasegawa, Daniel K. Kaur, Navneet Kliot, Adi Pinheiro, Patricia Valle Luan, Junbo Stensmyr, Marcus C. Zheng, Yi Liu, Wenli Sun, Honghe Xu, Yimin Luo, Yuan Kruse, Angela Yang, Xiaowei Kontsedalov, Svetlana Lebedev, Galina Fisher, Tonja W. Nelson, David R. Hunter, Wayne B. Brown, Judith K. Jander, Georg Cilia, Michelle Douglas, Angela E. Ghanim, Murad Simmons, Alvin M. Wintermantel, William M. Ling, Kai-Shu Fei, Zhangjun The draft genome of whitefly Bemisia tabaci MEAM1, a global crop pest, provides novel insights into virus transmission, host adaptation, and insecticide resistance |
title | The draft genome of whitefly Bemisia tabaci MEAM1, a global crop pest, provides novel insights into virus transmission, host adaptation, and insecticide resistance |
title_full | The draft genome of whitefly Bemisia tabaci MEAM1, a global crop pest, provides novel insights into virus transmission, host adaptation, and insecticide resistance |
title_fullStr | The draft genome of whitefly Bemisia tabaci MEAM1, a global crop pest, provides novel insights into virus transmission, host adaptation, and insecticide resistance |
title_full_unstemmed | The draft genome of whitefly Bemisia tabaci MEAM1, a global crop pest, provides novel insights into virus transmission, host adaptation, and insecticide resistance |
title_short | The draft genome of whitefly Bemisia tabaci MEAM1, a global crop pest, provides novel insights into virus transmission, host adaptation, and insecticide resistance |
title_sort | draft genome of whitefly bemisia tabaci meam1, a global crop pest, provides novel insights into virus transmission, host adaptation, and insecticide resistance |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5157087/ https://www.ncbi.nlm.nih.gov/pubmed/27974049 http://dx.doi.org/10.1186/s12915-016-0321-y |
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