Cargando…
Diversity of Methane-Oxidizing Bacteria in Soils from “Hot Lands of Medolla” (Italy) Featured by Anomalous High-Temperatures and Biogenic CO(2) Emission
“Terre Calde di Medolla” (TCM) (literally, “Hot Lands of Medolla”) refers to a farming area in Italy with anomalously high temperatures and diffuse emissions of biogenic CO(2), which has been linked to CH(4) oxidation processes from a depth of 0.7 m to the surface. We herein assessed the composition...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
the Japanese Society of Microbial Ecology (JSME)/the Japanese Society of Soil Microbiology (JSSM)/the Taiwan Society of Microbial Ecology (TSME)/the Japanese Society of Plant Microbe Interactions (JSPMI)
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5158108/ https://www.ncbi.nlm.nih.gov/pubmed/27645100 http://dx.doi.org/10.1264/jsme2.ME16087 |
_version_ | 1782481566964908032 |
---|---|
author | Cappelletti, Martina Ghezzi, Daniele Zannoni, Davide Capaccioni, Bruno Fedi, Stefano |
author_facet | Cappelletti, Martina Ghezzi, Daniele Zannoni, Davide Capaccioni, Bruno Fedi, Stefano |
author_sort | Cappelletti, Martina |
collection | PubMed |
description | “Terre Calde di Medolla” (TCM) (literally, “Hot Lands of Medolla”) refers to a farming area in Italy with anomalously high temperatures and diffuse emissions of biogenic CO(2), which has been linked to CH(4) oxidation processes from a depth of 0.7 m to the surface. We herein assessed the composition of the total bacterial community and diversity of methane-oxidizing bacteria (MOB) in soil samples collected at a depth at which the peak temperature was detected (0.6 m). Cultivation-independent methods were used, such as: i) a clone library analysis of the 16S rRNA gene and pmoA (coding for the α-subunit of the particulate methane monooxygenase) gene, and ii) Terminal Restriction Fragment Length Polymorphism (T-RFLP) fingerprinting. The 16S rRNA gene analysis assessed the predominance of Actinobacteria, Acidobacteria, Proteobacteria, and Bacillus in TCM samples collected at a depth of 0.6 m along with the presence of methanotrophs (Methylocaldum and Methylobacter) and methylotrophs (Methylobacillus). The phylogenetic analysis of pmoA sequences showed the presence of MOB affiliated with Methylomonas, Methylocystis, Methylococcus, and Methylocaldum in addition to as yet uncultivated and uncharacterized methanotrophs. Jaccard’s analysis of T-RFLP profiles at different ground depths revealed a similar MOB composition in soil samples at depths of 0.6 m and 0.7 m, while this similarity was weaker between these samples and those taken at a depth of 2.5 m, in which the genus Methylocaldum was absent. These results correlate the anomalously high temperatures of the farming area of “Terre Calde di Medolla” with the presence of microbial methane-oxidizing bacteria. |
format | Online Article Text |
id | pubmed-5158108 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | the Japanese Society of Microbial Ecology (JSME)/the Japanese Society of Soil Microbiology (JSSM)/the Taiwan Society of Microbial Ecology (TSME)/the Japanese Society of Plant Microbe Interactions (JSPMI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-51581082016-12-20 Diversity of Methane-Oxidizing Bacteria in Soils from “Hot Lands of Medolla” (Italy) Featured by Anomalous High-Temperatures and Biogenic CO(2) Emission Cappelletti, Martina Ghezzi, Daniele Zannoni, Davide Capaccioni, Bruno Fedi, Stefano Microbes Environ Articles “Terre Calde di Medolla” (TCM) (literally, “Hot Lands of Medolla”) refers to a farming area in Italy with anomalously high temperatures and diffuse emissions of biogenic CO(2), which has been linked to CH(4) oxidation processes from a depth of 0.7 m to the surface. We herein assessed the composition of the total bacterial community and diversity of methane-oxidizing bacteria (MOB) in soil samples collected at a depth at which the peak temperature was detected (0.6 m). Cultivation-independent methods were used, such as: i) a clone library analysis of the 16S rRNA gene and pmoA (coding for the α-subunit of the particulate methane monooxygenase) gene, and ii) Terminal Restriction Fragment Length Polymorphism (T-RFLP) fingerprinting. The 16S rRNA gene analysis assessed the predominance of Actinobacteria, Acidobacteria, Proteobacteria, and Bacillus in TCM samples collected at a depth of 0.6 m along with the presence of methanotrophs (Methylocaldum and Methylobacter) and methylotrophs (Methylobacillus). The phylogenetic analysis of pmoA sequences showed the presence of MOB affiliated with Methylomonas, Methylocystis, Methylococcus, and Methylocaldum in addition to as yet uncultivated and uncharacterized methanotrophs. Jaccard’s analysis of T-RFLP profiles at different ground depths revealed a similar MOB composition in soil samples at depths of 0.6 m and 0.7 m, while this similarity was weaker between these samples and those taken at a depth of 2.5 m, in which the genus Methylocaldum was absent. These results correlate the anomalously high temperatures of the farming area of “Terre Calde di Medolla” with the presence of microbial methane-oxidizing bacteria. the Japanese Society of Microbial Ecology (JSME)/the Japanese Society of Soil Microbiology (JSSM)/the Taiwan Society of Microbial Ecology (TSME)/the Japanese Society of Plant Microbe Interactions (JSPMI) 2016-12 2016-09-17 /pmc/articles/PMC5158108/ /pubmed/27645100 http://dx.doi.org/10.1264/jsme2.ME16087 Text en Copyright © 2016 by Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions. http://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Articles Cappelletti, Martina Ghezzi, Daniele Zannoni, Davide Capaccioni, Bruno Fedi, Stefano Diversity of Methane-Oxidizing Bacteria in Soils from “Hot Lands of Medolla” (Italy) Featured by Anomalous High-Temperatures and Biogenic CO(2) Emission |
title | Diversity of Methane-Oxidizing Bacteria in Soils from “Hot Lands of Medolla” (Italy) Featured by Anomalous High-Temperatures and Biogenic CO(2) Emission |
title_full | Diversity of Methane-Oxidizing Bacteria in Soils from “Hot Lands of Medolla” (Italy) Featured by Anomalous High-Temperatures and Biogenic CO(2) Emission |
title_fullStr | Diversity of Methane-Oxidizing Bacteria in Soils from “Hot Lands of Medolla” (Italy) Featured by Anomalous High-Temperatures and Biogenic CO(2) Emission |
title_full_unstemmed | Diversity of Methane-Oxidizing Bacteria in Soils from “Hot Lands of Medolla” (Italy) Featured by Anomalous High-Temperatures and Biogenic CO(2) Emission |
title_short | Diversity of Methane-Oxidizing Bacteria in Soils from “Hot Lands of Medolla” (Italy) Featured by Anomalous High-Temperatures and Biogenic CO(2) Emission |
title_sort | diversity of methane-oxidizing bacteria in soils from “hot lands of medolla” (italy) featured by anomalous high-temperatures and biogenic co(2) emission |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5158108/ https://www.ncbi.nlm.nih.gov/pubmed/27645100 http://dx.doi.org/10.1264/jsme2.ME16087 |
work_keys_str_mv | AT cappellettimartina diversityofmethaneoxidizingbacteriainsoilsfromhotlandsofmedollaitalyfeaturedbyanomaloushightemperaturesandbiogenicco2emission AT ghezzidaniele diversityofmethaneoxidizingbacteriainsoilsfromhotlandsofmedollaitalyfeaturedbyanomaloushightemperaturesandbiogenicco2emission AT zannonidavide diversityofmethaneoxidizingbacteriainsoilsfromhotlandsofmedollaitalyfeaturedbyanomaloushightemperaturesandbiogenicco2emission AT capaccionibruno diversityofmethaneoxidizingbacteriainsoilsfromhotlandsofmedollaitalyfeaturedbyanomaloushightemperaturesandbiogenicco2emission AT fedistefano diversityofmethaneoxidizingbacteriainsoilsfromhotlandsofmedollaitalyfeaturedbyanomaloushightemperaturesandbiogenicco2emission |