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Altered stoichiometry Escherichia coli Cascade complexes with shortened CRISPR RNA spacers are capable of interference and primed adaptation

The Escherichia coli type I-E CRISPR-Cas system Cascade effector is a multisubunit complex that binds CRISPR RNA (crRNA). Through its 32-nucleotide spacer sequence, Cascade-bound crRNA recognizes protospacers in foreign DNA, causing its destruction during CRISPR interference or acquisition of additi...

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Detalles Bibliográficos
Autores principales:	Kuznedelov, Konstantin, Mekler, Vladimir, Lemak, Sofia, Tokmina-Lukaszewska, Monika, Datsenko, Kirill A., Jain, Ishita, Savitskaya, Ekaterina, Mallon, John, Shmakov, Sergey, Bothner, Brian, Bailey, Scott, Yakunin, Alexander F., Severinov, Konstantin, Semenova, Ekaterina
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2016
Materias:	Nucleic Acid Enzymes
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5159557/ https://www.ncbi.nlm.nih.gov/pubmed/27738137 http://dx.doi.org/10.1093/nar/gkw914

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