CRISPR/Cas9 with single guide RNA expression driven by small tRNA promoters showed reduced editing efficiency compared to a U6 promoter

Multiplex genome engineering in vivo with CRISPR/Cas9 shows great promise as a potential therapeutic approach. The ability to incorporate multiple single guide RNA (sgRNA) cassettes together with Cas9 gene expression in one AAV vector could greatly enhance the efficiency. In a recent Method article,...

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Detalles Bibliográficos
Autores principales: Wei, Yuda, Qiu, Yan, Chen, Yanhao, Liu, Gaigai, Zhang, Yongxian, Xu, Luwei, Ding, Qiurong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2017
Materias:
Letter to the Editor
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5159642/
https://www.ncbi.nlm.nih.gov/pubmed/27742910
http://dx.doi.org/10.1261/rna.057596.116

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