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Role of proteasome-dependent protein degradation in long-term operant memory in Aplysia
We investigated the in vivo role of protein degradation during intermediate (ITM) and long-term memory (LTM) in Aplysia using an operant learning paradigm. The proteasome inhibitor MG-132 inhibited the induction and molecular consolidation of LTM with no effect on ITM. Remarkably, maintenance of ste...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory Press
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5159658/ https://www.ncbi.nlm.nih.gov/pubmed/27980077 http://dx.doi.org/10.1101/lm.043794.116 |
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author | Lyons, Lisa C. Gardner, Jacob S. Gandour, Catherine E. Krishnan, Harini C. |
author_facet | Lyons, Lisa C. Gardner, Jacob S. Gandour, Catherine E. Krishnan, Harini C. |
author_sort | Lyons, Lisa C. |
collection | PubMed |
description | We investigated the in vivo role of protein degradation during intermediate (ITM) and long-term memory (LTM) in Aplysia using an operant learning paradigm. The proteasome inhibitor MG-132 inhibited the induction and molecular consolidation of LTM with no effect on ITM. Remarkably, maintenance of steady-state protein levels through inhibition of protein synthesis using either anisomycin or rapamycin in conjunction with proteasome inhibition permitted the formation of robust 24 h LTM. Our studies suggest a primary role for proteasomal activity in facilitation of gene transcription for LTM and raise the possibility that synaptic mechanisms are sufficient to sustain 24 h memory. |
format | Online Article Text |
id | pubmed-5159658 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Cold Spring Harbor Laboratory Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-51596582018-01-01 Role of proteasome-dependent protein degradation in long-term operant memory in Aplysia Lyons, Lisa C. Gardner, Jacob S. Gandour, Catherine E. Krishnan, Harini C. Learn Mem Brief Communication We investigated the in vivo role of protein degradation during intermediate (ITM) and long-term memory (LTM) in Aplysia using an operant learning paradigm. The proteasome inhibitor MG-132 inhibited the induction and molecular consolidation of LTM with no effect on ITM. Remarkably, maintenance of steady-state protein levels through inhibition of protein synthesis using either anisomycin or rapamycin in conjunction with proteasome inhibition permitted the formation of robust 24 h LTM. Our studies suggest a primary role for proteasomal activity in facilitation of gene transcription for LTM and raise the possibility that synaptic mechanisms are sufficient to sustain 24 h memory. Cold Spring Harbor Laboratory Press 2017-01 /pmc/articles/PMC5159658/ /pubmed/27980077 http://dx.doi.org/10.1101/lm.043794.116 Text en © 2016 Lyons et al.; Published by Cold Spring Harbor Laboratory Press http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first 12 months after the full-issue publication date (see http://learnmem.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/. |
spellingShingle | Brief Communication Lyons, Lisa C. Gardner, Jacob S. Gandour, Catherine E. Krishnan, Harini C. Role of proteasome-dependent protein degradation in long-term operant memory in Aplysia |
title | Role of proteasome-dependent protein degradation in long-term operant memory in Aplysia |
title_full | Role of proteasome-dependent protein degradation in long-term operant memory in Aplysia |
title_fullStr | Role of proteasome-dependent protein degradation in long-term operant memory in Aplysia |
title_full_unstemmed | Role of proteasome-dependent protein degradation in long-term operant memory in Aplysia |
title_short | Role of proteasome-dependent protein degradation in long-term operant memory in Aplysia |
title_sort | role of proteasome-dependent protein degradation in long-term operant memory in aplysia |
topic | Brief Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5159658/ https://www.ncbi.nlm.nih.gov/pubmed/27980077 http://dx.doi.org/10.1101/lm.043794.116 |
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