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Brown adipogenic potential of brown adipocytes and peri-renal adipocytes from human embryo

Both brown adipocytes (BAC) and beige cells hold therapeutic potential for the treatment of metabolic disorders. Unfortunately, the amount and activity of these cells are limited in adults. Although BAC marker expression has been shown in peri-renal adipose tissues in children and adults, functional...

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Autores principales: Wu, Nan-Nan, Zhang, Chuan-Hai, Lee, Hyuek-Jong, Ma, Yan, Wang, Xin, Ma, Xiao-Juan, Ma, Wei, Zhao, Dong, Feng, Ying-Mei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5159842/
https://www.ncbi.nlm.nih.gov/pubmed/27982067
http://dx.doi.org/10.1038/srep39193
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author Wu, Nan-Nan
Zhang, Chuan-Hai
Lee, Hyuek-Jong
Ma, Yan
Wang, Xin
Ma, Xiao-Juan
Ma, Wei
Zhao, Dong
Feng, Ying-Mei
author_facet Wu, Nan-Nan
Zhang, Chuan-Hai
Lee, Hyuek-Jong
Ma, Yan
Wang, Xin
Ma, Xiao-Juan
Ma, Wei
Zhao, Dong
Feng, Ying-Mei
author_sort Wu, Nan-Nan
collection PubMed
description Both brown adipocytes (BAC) and beige cells hold therapeutic potential for the treatment of metabolic disorders. Unfortunately, the amount and activity of these cells are limited in adults. Although BAC marker expression has been shown in peri-renal adipose tissues in children and adults, functional assessment is lacking. Furthermore, it is entirely unknown whether adipose progenitors are present in human embryo and able to give rise to BAC in situ during evolution. Therefore, adipose tissues in the interscapular and peri-renal regions were dissected from human embryo and subcutaneous white adipose tissues (sWAT) were obtained from an adult. After subjected to differentiation in vitro, adipocyte progenitors were detected present in all these adipose tissues. When stimulated for adipogenesis, differentiated adipocytes in the intercapular and peri-renal regions showed similar features: (1) induced BAC and beige cell marker expression including UCP1 and PRDM16 and comparable mitochondrion copy number; (2) similar gene expression patterns by RNA-Seq analysis; and (3) similar maximal oxygen consumption rates examined by respirometry. Nevertheless, stimulation of adipocyte progenitors in sWAT induces neither BAC and beige cell marker expression nor any change of oxygen consumption. In conclusion, peri-renal adipocyte progenitors in human embryo hold browning potential for BAC production.
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spelling pubmed-51598422016-12-21 Brown adipogenic potential of brown adipocytes and peri-renal adipocytes from human embryo Wu, Nan-Nan Zhang, Chuan-Hai Lee, Hyuek-Jong Ma, Yan Wang, Xin Ma, Xiao-Juan Ma, Wei Zhao, Dong Feng, Ying-Mei Sci Rep Article Both brown adipocytes (BAC) and beige cells hold therapeutic potential for the treatment of metabolic disorders. Unfortunately, the amount and activity of these cells are limited in adults. Although BAC marker expression has been shown in peri-renal adipose tissues in children and adults, functional assessment is lacking. Furthermore, it is entirely unknown whether adipose progenitors are present in human embryo and able to give rise to BAC in situ during evolution. Therefore, adipose tissues in the interscapular and peri-renal regions were dissected from human embryo and subcutaneous white adipose tissues (sWAT) were obtained from an adult. After subjected to differentiation in vitro, adipocyte progenitors were detected present in all these adipose tissues. When stimulated for adipogenesis, differentiated adipocytes in the intercapular and peri-renal regions showed similar features: (1) induced BAC and beige cell marker expression including UCP1 and PRDM16 and comparable mitochondrion copy number; (2) similar gene expression patterns by RNA-Seq analysis; and (3) similar maximal oxygen consumption rates examined by respirometry. Nevertheless, stimulation of adipocyte progenitors in sWAT induces neither BAC and beige cell marker expression nor any change of oxygen consumption. In conclusion, peri-renal adipocyte progenitors in human embryo hold browning potential for BAC production. Nature Publishing Group 2016-12-16 /pmc/articles/PMC5159842/ /pubmed/27982067 http://dx.doi.org/10.1038/srep39193 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Wu, Nan-Nan
Zhang, Chuan-Hai
Lee, Hyuek-Jong
Ma, Yan
Wang, Xin
Ma, Xiao-Juan
Ma, Wei
Zhao, Dong
Feng, Ying-Mei
Brown adipogenic potential of brown adipocytes and peri-renal adipocytes from human embryo
title Brown adipogenic potential of brown adipocytes and peri-renal adipocytes from human embryo
title_full Brown adipogenic potential of brown adipocytes and peri-renal adipocytes from human embryo
title_fullStr Brown adipogenic potential of brown adipocytes and peri-renal adipocytes from human embryo
title_full_unstemmed Brown adipogenic potential of brown adipocytes and peri-renal adipocytes from human embryo
title_short Brown adipogenic potential of brown adipocytes and peri-renal adipocytes from human embryo
title_sort brown adipogenic potential of brown adipocytes and peri-renal adipocytes from human embryo
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5159842/
https://www.ncbi.nlm.nih.gov/pubmed/27982067
http://dx.doi.org/10.1038/srep39193
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