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Multicenter evaluation of crystal violet decolorization assay (CVDA) for rapid detection of isoniazid and rifampicin resistance in Mycobacterium tuberculosis
The aim of this multicenter study was to evaluate the performance of the crystal violet decolorization assay (CVDA) for detection of multidrug resistant tuberculosis (MDR-TB). This study was performed in 11 centers in two phases. A total of 156 isolates were tested for INH and RIF resistance. In the...
Autores principales: | , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5159858/ https://www.ncbi.nlm.nih.gov/pubmed/27982061 http://dx.doi.org/10.1038/srep39050 |
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author | Coban, Ahmet Yilmaz Akbal, Ahmet Ugur Bicmen, Can Albay, Ali Sig, Ali Korhan Uzun, Meltem Selale, Deniz Sertel Ozkutuk, Nuri Surucuoglu, Suheyla Albayrak, Nurhan Ucarman, Nilay Ozkutuk, Aydan Esen, Nuran Ceyhan, Ismail Ozyurt, Mustafa Bektore, Bayhan Aslan, Gonul Delialioğlu, Nuran Alp, Alpaslan |
author_facet | Coban, Ahmet Yilmaz Akbal, Ahmet Ugur Bicmen, Can Albay, Ali Sig, Ali Korhan Uzun, Meltem Selale, Deniz Sertel Ozkutuk, Nuri Surucuoglu, Suheyla Albayrak, Nurhan Ucarman, Nilay Ozkutuk, Aydan Esen, Nuran Ceyhan, Ismail Ozyurt, Mustafa Bektore, Bayhan Aslan, Gonul Delialioğlu, Nuran Alp, Alpaslan |
author_sort | Coban, Ahmet Yilmaz |
collection | PubMed |
description | The aim of this multicenter study was to evaluate the performance of the crystal violet decolorization assay (CVDA) for detection of multidrug resistant tuberculosis (MDR-TB). This study was performed in 11 centers in two phases. A total of 156 isolates were tested for INH and RIF resistance. In the phase I, 106 clinical isolates were tested in the Center 1–7. In the phase 2, 156 clinical isolates were tested in the center 1–6, center 8–11. Eighty six of 156 tested isolates were the same in phase I. Agreements were 96.2–96.8% for INH and 98.1–98.7% for RIF in the phase I-II, respectively. Mean time to obtain the results in the phase I was 14.3 ± 5.4 days. In the phase II, mean time to obtain the results was 11.6 ± 3.5 days. Test results were obtained within 14days for 62.3% (66/106) of isolates in the phase I and 81.4% (127/156) of isolates in the phase II. In conclusion, CVDA is rapid, reliable, inexpensive, and easy to perform for rapid detection of MDR-TB isolates. In addition, it could be adapted for drug susceptibility testing with all drugs both in developed and developing countries. |
format | Online Article Text |
id | pubmed-5159858 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-51598582016-12-21 Multicenter evaluation of crystal violet decolorization assay (CVDA) for rapid detection of isoniazid and rifampicin resistance in Mycobacterium tuberculosis Coban, Ahmet Yilmaz Akbal, Ahmet Ugur Bicmen, Can Albay, Ali Sig, Ali Korhan Uzun, Meltem Selale, Deniz Sertel Ozkutuk, Nuri Surucuoglu, Suheyla Albayrak, Nurhan Ucarman, Nilay Ozkutuk, Aydan Esen, Nuran Ceyhan, Ismail Ozyurt, Mustafa Bektore, Bayhan Aslan, Gonul Delialioğlu, Nuran Alp, Alpaslan Sci Rep Article The aim of this multicenter study was to evaluate the performance of the crystal violet decolorization assay (CVDA) for detection of multidrug resistant tuberculosis (MDR-TB). This study was performed in 11 centers in two phases. A total of 156 isolates were tested for INH and RIF resistance. In the phase I, 106 clinical isolates were tested in the Center 1–7. In the phase 2, 156 clinical isolates were tested in the center 1–6, center 8–11. Eighty six of 156 tested isolates were the same in phase I. Agreements were 96.2–96.8% for INH and 98.1–98.7% for RIF in the phase I-II, respectively. Mean time to obtain the results in the phase I was 14.3 ± 5.4 days. In the phase II, mean time to obtain the results was 11.6 ± 3.5 days. Test results were obtained within 14days for 62.3% (66/106) of isolates in the phase I and 81.4% (127/156) of isolates in the phase II. In conclusion, CVDA is rapid, reliable, inexpensive, and easy to perform for rapid detection of MDR-TB isolates. In addition, it could be adapted for drug susceptibility testing with all drugs both in developed and developing countries. Nature Publishing Group 2016-12-16 /pmc/articles/PMC5159858/ /pubmed/27982061 http://dx.doi.org/10.1038/srep39050 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Coban, Ahmet Yilmaz Akbal, Ahmet Ugur Bicmen, Can Albay, Ali Sig, Ali Korhan Uzun, Meltem Selale, Deniz Sertel Ozkutuk, Nuri Surucuoglu, Suheyla Albayrak, Nurhan Ucarman, Nilay Ozkutuk, Aydan Esen, Nuran Ceyhan, Ismail Ozyurt, Mustafa Bektore, Bayhan Aslan, Gonul Delialioğlu, Nuran Alp, Alpaslan Multicenter evaluation of crystal violet decolorization assay (CVDA) for rapid detection of isoniazid and rifampicin resistance in Mycobacterium tuberculosis |
title | Multicenter evaluation of crystal violet decolorization assay (CVDA) for rapid detection of isoniazid and rifampicin resistance in Mycobacterium tuberculosis |
title_full | Multicenter evaluation of crystal violet decolorization assay (CVDA) for rapid detection of isoniazid and rifampicin resistance in Mycobacterium tuberculosis |
title_fullStr | Multicenter evaluation of crystal violet decolorization assay (CVDA) for rapid detection of isoniazid and rifampicin resistance in Mycobacterium tuberculosis |
title_full_unstemmed | Multicenter evaluation of crystal violet decolorization assay (CVDA) for rapid detection of isoniazid and rifampicin resistance in Mycobacterium tuberculosis |
title_short | Multicenter evaluation of crystal violet decolorization assay (CVDA) for rapid detection of isoniazid and rifampicin resistance in Mycobacterium tuberculosis |
title_sort | multicenter evaluation of crystal violet decolorization assay (cvda) for rapid detection of isoniazid and rifampicin resistance in mycobacterium tuberculosis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5159858/ https://www.ncbi.nlm.nih.gov/pubmed/27982061 http://dx.doi.org/10.1038/srep39050 |
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