EPSPS Gene Copy Number and Whole-Plant Glyphosate Resistance Level in Kochia scoparia

Glyphosate-resistant (GR) Kochia scoparia has evolved in dryland chemical fallow systems throughout North America and the mechanism of resistance involves 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene duplication. Agricultural fields in four states were surveyed for K. scoparia in 2013 an...

Descripción completa

Detalles Bibliográficos
Autores principales: Gaines, Todd A., Barker, Abigail L., Patterson, Eric L., Westra, Philip, Westra, Eric P., Wilson, Robert G., Jha, Prashant, Kumar, Vipan, Kniss, Andrew R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5161467/
https://www.ncbi.nlm.nih.gov/pubmed/27992501
http://dx.doi.org/10.1371/journal.pone.0168295
_version_ 1782482085499371520
author Gaines, Todd A.
Barker, Abigail L.
Patterson, Eric L.
Westra, Philip
Westra, Eric P.
Wilson, Robert G.
Jha, Prashant
Kumar, Vipan
Kniss, Andrew R.
author_facet Gaines, Todd A.
Barker, Abigail L.
Patterson, Eric L.
Westra, Philip
Westra, Eric P.
Wilson, Robert G.
Jha, Prashant
Kumar, Vipan
Kniss, Andrew R.
author_sort Gaines, Todd A.
collection PubMed
description Glyphosate-resistant (GR) Kochia scoparia has evolved in dryland chemical fallow systems throughout North America and the mechanism of resistance involves 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene duplication. Agricultural fields in four states were surveyed for K. scoparia in 2013 and tested for glyphosate-resistance level and EPSPS gene copy number. Glyphosate resistance was confirmed in K. scoparia populations collected from sugarbeet fields in Colorado, Wyoming, and Nebraska, and Montana. Glyphosate resistance was also confirmed in K. scoparia accessions collected from wheat-fallow fields in Montana. All GR samples had increased EPSPS gene copy number, with median population values up to 11 from sugarbeet fields and up to 13 in Montana wheat-fallow fields. The results indicate that glyphosate susceptibility can be accurately diagnosed using EPSPS gene copy number.
format Online
Article
Text
id pubmed-5161467
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-51614672017-01-04 EPSPS Gene Copy Number and Whole-Plant Glyphosate Resistance Level in Kochia scoparia Gaines, Todd A. Barker, Abigail L. Patterson, Eric L. Westra, Philip Westra, Eric P. Wilson, Robert G. Jha, Prashant Kumar, Vipan Kniss, Andrew R. PLoS One Research Article Glyphosate-resistant (GR) Kochia scoparia has evolved in dryland chemical fallow systems throughout North America and the mechanism of resistance involves 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene duplication. Agricultural fields in four states were surveyed for K. scoparia in 2013 and tested for glyphosate-resistance level and EPSPS gene copy number. Glyphosate resistance was confirmed in K. scoparia populations collected from sugarbeet fields in Colorado, Wyoming, and Nebraska, and Montana. Glyphosate resistance was also confirmed in K. scoparia accessions collected from wheat-fallow fields in Montana. All GR samples had increased EPSPS gene copy number, with median population values up to 11 from sugarbeet fields and up to 13 in Montana wheat-fallow fields. The results indicate that glyphosate susceptibility can be accurately diagnosed using EPSPS gene copy number. Public Library of Science 2016-12-16 /pmc/articles/PMC5161467/ /pubmed/27992501 http://dx.doi.org/10.1371/journal.pone.0168295 Text en © 2016 Gaines et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Gaines, Todd A.
Barker, Abigail L.
Patterson, Eric L.
Westra, Philip
Westra, Eric P.
Wilson, Robert G.
Jha, Prashant
Kumar, Vipan
Kniss, Andrew R.
EPSPS Gene Copy Number and Whole-Plant Glyphosate Resistance Level in Kochia scoparia
title EPSPS Gene Copy Number and Whole-Plant Glyphosate Resistance Level in Kochia scoparia
title_full EPSPS Gene Copy Number and Whole-Plant Glyphosate Resistance Level in Kochia scoparia
title_fullStr EPSPS Gene Copy Number and Whole-Plant Glyphosate Resistance Level in Kochia scoparia
title_full_unstemmed EPSPS Gene Copy Number and Whole-Plant Glyphosate Resistance Level in Kochia scoparia
title_short EPSPS Gene Copy Number and Whole-Plant Glyphosate Resistance Level in Kochia scoparia
title_sort epsps gene copy number and whole-plant glyphosate resistance level in kochia scoparia
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5161467/
https://www.ncbi.nlm.nih.gov/pubmed/27992501
http://dx.doi.org/10.1371/journal.pone.0168295
work_keys_str_mv AT gainestodda epspsgenecopynumberandwholeplantglyphosateresistancelevelinkochiascoparia
AT barkerabigaill epspsgenecopynumberandwholeplantglyphosateresistancelevelinkochiascoparia
AT pattersonericl epspsgenecopynumberandwholeplantglyphosateresistancelevelinkochiascoparia
AT westraphilip epspsgenecopynumberandwholeplantglyphosateresistancelevelinkochiascoparia
AT westraericp epspsgenecopynumberandwholeplantglyphosateresistancelevelinkochiascoparia
AT wilsonrobertg epspsgenecopynumberandwholeplantglyphosateresistancelevelinkochiascoparia
AT jhaprashant epspsgenecopynumberandwholeplantglyphosateresistancelevelinkochiascoparia
AT kumarvipan epspsgenecopynumberandwholeplantglyphosateresistancelevelinkochiascoparia
AT knissandrewr epspsgenecopynumberandwholeplantglyphosateresistancelevelinkochiascoparia

Ejemplares similares