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USP10 Is an Essential Deubiquitinase for Hematopoiesis and Inhibits Apoptosis of Long-Term Hematopoietic Stem Cells
Self-renewal, replication, and differentiation of hematopoietic stem cells (HSCs) are regulated by cytokines produced by niche cells in fetal liver and bone marrow. HSCs must overcome stresses induced by cytokine deprivation during normal development. In this study, we found that ubiquitin-specific...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5161743/ https://www.ncbi.nlm.nih.gov/pubmed/27974222 http://dx.doi.org/10.1016/j.stemcr.2016.11.003 |
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author | Higuchi, Masaya Kawamura, Hiroki Matsuki, Hideaki Hara, Toshifumi Takahashi, Masahiko Saito, Suguru Saito, Kousuke Jiang, Shuying Naito, Makoto Kiyonari, Hiroshi Fujii, Masahiro |
author_facet | Higuchi, Masaya Kawamura, Hiroki Matsuki, Hideaki Hara, Toshifumi Takahashi, Masahiko Saito, Suguru Saito, Kousuke Jiang, Shuying Naito, Makoto Kiyonari, Hiroshi Fujii, Masahiro |
author_sort | Higuchi, Masaya |
collection | PubMed |
description | Self-renewal, replication, and differentiation of hematopoietic stem cells (HSCs) are regulated by cytokines produced by niche cells in fetal liver and bone marrow. HSCs must overcome stresses induced by cytokine deprivation during normal development. In this study, we found that ubiquitin-specific peptidase 10 (USP10) is a crucial deubiquitinase for mouse hematopoiesis. All USP10 knockout (KO) mice died within 1 year because of bone marrow failure with pancytopenia. Bone marrow failure in these USP10-KO mice was associated with remarkable reductions of long-term HSCs (LT-HSCs) in bone marrow and fetal liver. Such USP10-KO fetal liver exhibited enhanced apoptosis of hematopoietic stem/progenitor cells (HSPCs) including LT-HSCs but not of lineage-committed progenitor cells. Transplantation of USP10-competent bone marrow cells into USP10-KO mice reconstituted multilineage hematopoiesis. These results suggest that USP10 is an essential deubiquitinase in hematopoiesis and functions by inhibiting apoptosis of HSPCs including LT-HSCs. |
format | Online Article Text |
id | pubmed-5161743 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-51617432016-12-21 USP10 Is an Essential Deubiquitinase for Hematopoiesis and Inhibits Apoptosis of Long-Term Hematopoietic Stem Cells Higuchi, Masaya Kawamura, Hiroki Matsuki, Hideaki Hara, Toshifumi Takahashi, Masahiko Saito, Suguru Saito, Kousuke Jiang, Shuying Naito, Makoto Kiyonari, Hiroshi Fujii, Masahiro Stem Cell Reports Article Self-renewal, replication, and differentiation of hematopoietic stem cells (HSCs) are regulated by cytokines produced by niche cells in fetal liver and bone marrow. HSCs must overcome stresses induced by cytokine deprivation during normal development. In this study, we found that ubiquitin-specific peptidase 10 (USP10) is a crucial deubiquitinase for mouse hematopoiesis. All USP10 knockout (KO) mice died within 1 year because of bone marrow failure with pancytopenia. Bone marrow failure in these USP10-KO mice was associated with remarkable reductions of long-term HSCs (LT-HSCs) in bone marrow and fetal liver. Such USP10-KO fetal liver exhibited enhanced apoptosis of hematopoietic stem/progenitor cells (HSPCs) including LT-HSCs but not of lineage-committed progenitor cells. Transplantation of USP10-competent bone marrow cells into USP10-KO mice reconstituted multilineage hematopoiesis. These results suggest that USP10 is an essential deubiquitinase in hematopoiesis and functions by inhibiting apoptosis of HSPCs including LT-HSCs. Elsevier 2016-12-13 /pmc/articles/PMC5161743/ /pubmed/27974222 http://dx.doi.org/10.1016/j.stemcr.2016.11.003 Text en © 2016 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Higuchi, Masaya Kawamura, Hiroki Matsuki, Hideaki Hara, Toshifumi Takahashi, Masahiko Saito, Suguru Saito, Kousuke Jiang, Shuying Naito, Makoto Kiyonari, Hiroshi Fujii, Masahiro USP10 Is an Essential Deubiquitinase for Hematopoiesis and Inhibits Apoptosis of Long-Term Hematopoietic Stem Cells |
title | USP10 Is an Essential Deubiquitinase for Hematopoiesis and Inhibits Apoptosis of Long-Term Hematopoietic Stem Cells |
title_full | USP10 Is an Essential Deubiquitinase for Hematopoiesis and Inhibits Apoptosis of Long-Term Hematopoietic Stem Cells |
title_fullStr | USP10 Is an Essential Deubiquitinase for Hematopoiesis and Inhibits Apoptosis of Long-Term Hematopoietic Stem Cells |
title_full_unstemmed | USP10 Is an Essential Deubiquitinase for Hematopoiesis and Inhibits Apoptosis of Long-Term Hematopoietic Stem Cells |
title_short | USP10 Is an Essential Deubiquitinase for Hematopoiesis and Inhibits Apoptosis of Long-Term Hematopoietic Stem Cells |
title_sort | usp10 is an essential deubiquitinase for hematopoiesis and inhibits apoptosis of long-term hematopoietic stem cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5161743/ https://www.ncbi.nlm.nih.gov/pubmed/27974222 http://dx.doi.org/10.1016/j.stemcr.2016.11.003 |
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