Cargando…
Dietary olive oil induces cannabinoid CB2 receptor expression in adipose tissue of Apc(Min/+) transgenic mice
BACKGROUND: Cannabinoid- 2 (CB2) receptor is known for its anti-obesity effects silencing the activated immune cells that are key drivers of metabolic syndrome and inflammation. Nutritional interventions in experimental models of carcinogenesis have been demonstrated to modulate tissue inflammation...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
IOS Press
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5166557/ https://www.ncbi.nlm.nih.gov/pubmed/28035344 http://dx.doi.org/10.3233/NHA-160008 |
_version_ | 1782483052102942720 |
---|---|
author | Notarnicola, Maria Tutino, Valeria Tafaro, Angela Bianco, Giusy Guglielmi, Emilia Caruso, Maria Gabriella |
author_facet | Notarnicola, Maria Tutino, Valeria Tafaro, Angela Bianco, Giusy Guglielmi, Emilia Caruso, Maria Gabriella |
author_sort | Notarnicola, Maria |
collection | PubMed |
description | BACKGROUND: Cannabinoid- 2 (CB2) receptor is known for its anti-obesity effects silencing the activated immune cells that are key drivers of metabolic syndrome and inflammation. Nutritional interventions in experimental models of carcinogenesis have been demonstrated to modulate tissue inflammation state and proliferation. OBJECTIVE: Aim of this study was to test, in Apc(Min/+) mice, whether a diet enriched with olive oil, omega- 3 and omega-6- PUFAs affects the adipose tissue inflammation status. METHODS: Four groups of animal were studied: ST group, receiving a standard diet; OO group, receiving the standard diet in which soybean oil (source of fats) was replaced with olive oil; OM-3 group, receiving the standard diet in which soybean oil was replaced with salmon oil; OM-6 group, receiving the standard diet in which soybean oil was replaced with oenothera oil. Gene and protein expression, in adipose tissue, were evaluated by RT-PCR and Western Blotting, respectively. Enzymatic activities were assayed by fluorescent and radiometric method, where appropriated. RESULTS: The diet enriched with olive oil significantly induced CB2 receptor expression and it was able to control inflammatory and proliferative activity of mice adipose tissue. CONCLUSIONS: The present findings open opportunities for developing novel nutritional strategies considering olive oil a key ingredient of a healthy dietary pattern. |
format | Online Article Text |
id | pubmed-5166557 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | IOS Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-51665572016-12-27 Dietary olive oil induces cannabinoid CB2 receptor expression in adipose tissue of Apc(Min/+) transgenic mice Notarnicola, Maria Tutino, Valeria Tafaro, Angela Bianco, Giusy Guglielmi, Emilia Caruso, Maria Gabriella Nutr Healthy Aging Research Report BACKGROUND: Cannabinoid- 2 (CB2) receptor is known for its anti-obesity effects silencing the activated immune cells that are key drivers of metabolic syndrome and inflammation. Nutritional interventions in experimental models of carcinogenesis have been demonstrated to modulate tissue inflammation state and proliferation. OBJECTIVE: Aim of this study was to test, in Apc(Min/+) mice, whether a diet enriched with olive oil, omega- 3 and omega-6- PUFAs affects the adipose tissue inflammation status. METHODS: Four groups of animal were studied: ST group, receiving a standard diet; OO group, receiving the standard diet in which soybean oil (source of fats) was replaced with olive oil; OM-3 group, receiving the standard diet in which soybean oil was replaced with salmon oil; OM-6 group, receiving the standard diet in which soybean oil was replaced with oenothera oil. Gene and protein expression, in adipose tissue, were evaluated by RT-PCR and Western Blotting, respectively. Enzymatic activities were assayed by fluorescent and radiometric method, where appropriated. RESULTS: The diet enriched with olive oil significantly induced CB2 receptor expression and it was able to control inflammatory and proliferative activity of mice adipose tissue. CONCLUSIONS: The present findings open opportunities for developing novel nutritional strategies considering olive oil a key ingredient of a healthy dietary pattern. IOS Press 2016-10-27 /pmc/articles/PMC5166557/ /pubmed/28035344 http://dx.doi.org/10.3233/NHA-160008 Text en IOS Press and the authors. All rights reserved https://creativecommons.org/licenses/by-nc/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial (CC BY-NC 4.0) License (https://creativecommons.org/licenses/by-nc/4.0/) , which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Report Notarnicola, Maria Tutino, Valeria Tafaro, Angela Bianco, Giusy Guglielmi, Emilia Caruso, Maria Gabriella Dietary olive oil induces cannabinoid CB2 receptor expression in adipose tissue of Apc(Min/+) transgenic mice |
title | Dietary olive oil induces cannabinoid CB2 receptor expression in adipose tissue of Apc(Min/+) transgenic mice |
title_full | Dietary olive oil induces cannabinoid CB2 receptor expression in adipose tissue of Apc(Min/+) transgenic mice |
title_fullStr | Dietary olive oil induces cannabinoid CB2 receptor expression in adipose tissue of Apc(Min/+) transgenic mice |
title_full_unstemmed | Dietary olive oil induces cannabinoid CB2 receptor expression in adipose tissue of Apc(Min/+) transgenic mice |
title_short | Dietary olive oil induces cannabinoid CB2 receptor expression in adipose tissue of Apc(Min/+) transgenic mice |
title_sort | dietary olive oil induces cannabinoid cb2 receptor expression in adipose tissue of apc(min/+) transgenic mice |
topic | Research Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5166557/ https://www.ncbi.nlm.nih.gov/pubmed/28035344 http://dx.doi.org/10.3233/NHA-160008 |
work_keys_str_mv | AT notarnicolamaria dietaryoliveoilinducescannabinoidcb2receptorexpressioninadiposetissueofapcmintransgenicmice AT tutinovaleria dietaryoliveoilinducescannabinoidcb2receptorexpressioninadiposetissueofapcmintransgenicmice AT tafaroangela dietaryoliveoilinducescannabinoidcb2receptorexpressioninadiposetissueofapcmintransgenicmice AT biancogiusy dietaryoliveoilinducescannabinoidcb2receptorexpressioninadiposetissueofapcmintransgenicmice AT guglielmiemilia dietaryoliveoilinducescannabinoidcb2receptorexpressioninadiposetissueofapcmintransgenicmice AT carusomariagabriella dietaryoliveoilinducescannabinoidcb2receptorexpressioninadiposetissueofapcmintransgenicmice |