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TECRL, a new life‐threatening inherited arrhythmia gene associated with overlapping clinical features of both LQTS and CPVT
Genetic causes of many familial arrhythmia syndromes remain elusive. In this study, whole‐exome sequencing (WES) was carried out on patients from three different families that presented with life‐threatening arrhythmias and high risk of sudden cardiac death (SCD). Two French Canadian probands carrie...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5167130/ https://www.ncbi.nlm.nih.gov/pubmed/27861123 http://dx.doi.org/10.15252/emmm.201505719 |
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author | Devalla, Harsha D Gélinas, Roselle Aburawi, Elhadi H Beqqali, Abdelaziz Goyette, Philippe Freund, Christian Chaix, Marie‐A Tadros, Rafik Jiang, Hui Le Béchec, Antony Monshouwer‐Kloots, Jantine J Zwetsloot, Tom Kosmidis, Georgios Latour, Frédéric Alikashani, Azadeh Hoekstra, Maaike Schlaepfer, Jurg Mummery, Christine L Stevenson, Brian Kutalik, Zoltan de Vries, Antoine AF Rivard, Léna Wilde, Arthur AM Talajic, Mario Verkerk, Arie O Al‐Gazali, Lihadh Rioux, John D Bhuiyan, Zahurul A Passier, Robert |
author_facet | Devalla, Harsha D Gélinas, Roselle Aburawi, Elhadi H Beqqali, Abdelaziz Goyette, Philippe Freund, Christian Chaix, Marie‐A Tadros, Rafik Jiang, Hui Le Béchec, Antony Monshouwer‐Kloots, Jantine J Zwetsloot, Tom Kosmidis, Georgios Latour, Frédéric Alikashani, Azadeh Hoekstra, Maaike Schlaepfer, Jurg Mummery, Christine L Stevenson, Brian Kutalik, Zoltan de Vries, Antoine AF Rivard, Léna Wilde, Arthur AM Talajic, Mario Verkerk, Arie O Al‐Gazali, Lihadh Rioux, John D Bhuiyan, Zahurul A Passier, Robert |
author_sort | Devalla, Harsha D |
collection | PubMed |
description | Genetic causes of many familial arrhythmia syndromes remain elusive. In this study, whole‐exome sequencing (WES) was carried out on patients from three different families that presented with life‐threatening arrhythmias and high risk of sudden cardiac death (SCD). Two French Canadian probands carried identical homozygous rare variant in TECRL gene (p.Arg196Gln), which encodes the trans‐2,3‐enoyl‐CoA reductase‐like protein. Both patients had cardiac arrest, stress‐induced atrial and ventricular tachycardia, and QT prolongation on adrenergic stimulation. A third patient from a consanguineous Sudanese family diagnosed with catecholaminergic polymorphic ventricular tachycardia (CPVT) had a homozygous splice site mutation (c.331+1G>A) in TECRL. Analysis of intracellular calcium ([Ca(2+)](i)) dynamics in human induced pluripotent stem cell‐derived cardiomyocytes (hiPSC‐CMs) generated from this individual (TECRL(H) (om)‐hiPSCs), his heterozygous but clinically asymptomatic father (TECRL(H) (et)‐hiPSCs), and a healthy individual (CTRL‐hiPSCs) from the same Sudanese family, revealed smaller [Ca(2+)](i) transient amplitudes as well as elevated diastolic [Ca(2+)](i) in TECRL(H) (om)‐hiPSC‐CMs compared with CTRL‐hiPSC‐CMs. The [Ca(2+)](i) transient also rose markedly slower and contained lower sarcoplasmic reticulum (SR) calcium stores, evidenced by the decreased magnitude of caffeine‐induced [Ca(2+)](i) transients. In addition, the decay phase of the [Ca(2+)](i) transient was slower in TECRL(H) (om)‐hiPSC‐CMs due to decreased SERCA and NCX activities. Furthermore, TECRL(H) (om)‐hiPSC‐CMs showed prolonged action potentials (APs) compared with CTRL‐hiPSC‐CMs. TECRL knockdown in control human embryonic stem cell‐derived CMs (hESC‐CMs) also resulted in significantly longer APs. Moreover, stimulation by noradrenaline (NA) significantly increased the propensity for triggered activity based on delayed afterdepolarizations (DADs) in TECRL(H) (om)‐hiPSC‐CMs and treatment with flecainide, a class Ic antiarrhythmic drug, significantly reduced the triggered activity in these cells. In summary, we report that mutations in TECRL are associated with inherited arrhythmias characterized by clinical features of both LQTS and CPVT. Patient‐specific hiPSC‐CMs recapitulated salient features of the clinical phenotype and provide a platform for drug screening evidenced by initial identification of flecainide as a potential therapeutic. These findings have implications for diagnosis and treatment of inherited cardiac arrhythmias. |
format | Online Article Text |
id | pubmed-5167130 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-51671302016-12-28 TECRL, a new life‐threatening inherited arrhythmia gene associated with overlapping clinical features of both LQTS and CPVT Devalla, Harsha D Gélinas, Roselle Aburawi, Elhadi H Beqqali, Abdelaziz Goyette, Philippe Freund, Christian Chaix, Marie‐A Tadros, Rafik Jiang, Hui Le Béchec, Antony Monshouwer‐Kloots, Jantine J Zwetsloot, Tom Kosmidis, Georgios Latour, Frédéric Alikashani, Azadeh Hoekstra, Maaike Schlaepfer, Jurg Mummery, Christine L Stevenson, Brian Kutalik, Zoltan de Vries, Antoine AF Rivard, Léna Wilde, Arthur AM Talajic, Mario Verkerk, Arie O Al‐Gazali, Lihadh Rioux, John D Bhuiyan, Zahurul A Passier, Robert EMBO Mol Med Research Articles Genetic causes of many familial arrhythmia syndromes remain elusive. In this study, whole‐exome sequencing (WES) was carried out on patients from three different families that presented with life‐threatening arrhythmias and high risk of sudden cardiac death (SCD). Two French Canadian probands carried identical homozygous rare variant in TECRL gene (p.Arg196Gln), which encodes the trans‐2,3‐enoyl‐CoA reductase‐like protein. Both patients had cardiac arrest, stress‐induced atrial and ventricular tachycardia, and QT prolongation on adrenergic stimulation. A third patient from a consanguineous Sudanese family diagnosed with catecholaminergic polymorphic ventricular tachycardia (CPVT) had a homozygous splice site mutation (c.331+1G>A) in TECRL. Analysis of intracellular calcium ([Ca(2+)](i)) dynamics in human induced pluripotent stem cell‐derived cardiomyocytes (hiPSC‐CMs) generated from this individual (TECRL(H) (om)‐hiPSCs), his heterozygous but clinically asymptomatic father (TECRL(H) (et)‐hiPSCs), and a healthy individual (CTRL‐hiPSCs) from the same Sudanese family, revealed smaller [Ca(2+)](i) transient amplitudes as well as elevated diastolic [Ca(2+)](i) in TECRL(H) (om)‐hiPSC‐CMs compared with CTRL‐hiPSC‐CMs. The [Ca(2+)](i) transient also rose markedly slower and contained lower sarcoplasmic reticulum (SR) calcium stores, evidenced by the decreased magnitude of caffeine‐induced [Ca(2+)](i) transients. In addition, the decay phase of the [Ca(2+)](i) transient was slower in TECRL(H) (om)‐hiPSC‐CMs due to decreased SERCA and NCX activities. Furthermore, TECRL(H) (om)‐hiPSC‐CMs showed prolonged action potentials (APs) compared with CTRL‐hiPSC‐CMs. TECRL knockdown in control human embryonic stem cell‐derived CMs (hESC‐CMs) also resulted in significantly longer APs. Moreover, stimulation by noradrenaline (NA) significantly increased the propensity for triggered activity based on delayed afterdepolarizations (DADs) in TECRL(H) (om)‐hiPSC‐CMs and treatment with flecainide, a class Ic antiarrhythmic drug, significantly reduced the triggered activity in these cells. In summary, we report that mutations in TECRL are associated with inherited arrhythmias characterized by clinical features of both LQTS and CPVT. Patient‐specific hiPSC‐CMs recapitulated salient features of the clinical phenotype and provide a platform for drug screening evidenced by initial identification of flecainide as a potential therapeutic. These findings have implications for diagnosis and treatment of inherited cardiac arrhythmias. John Wiley and Sons Inc. 2016-10-24 2016-12 /pmc/articles/PMC5167130/ /pubmed/27861123 http://dx.doi.org/10.15252/emmm.201505719 Text en © 2016 The Authors. Published under the terms of the CC BY 4.0 license This is an open access article under the terms of the Creative Commons Attribution 4.0 (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Devalla, Harsha D Gélinas, Roselle Aburawi, Elhadi H Beqqali, Abdelaziz Goyette, Philippe Freund, Christian Chaix, Marie‐A Tadros, Rafik Jiang, Hui Le Béchec, Antony Monshouwer‐Kloots, Jantine J Zwetsloot, Tom Kosmidis, Georgios Latour, Frédéric Alikashani, Azadeh Hoekstra, Maaike Schlaepfer, Jurg Mummery, Christine L Stevenson, Brian Kutalik, Zoltan de Vries, Antoine AF Rivard, Léna Wilde, Arthur AM Talajic, Mario Verkerk, Arie O Al‐Gazali, Lihadh Rioux, John D Bhuiyan, Zahurul A Passier, Robert TECRL, a new life‐threatening inherited arrhythmia gene associated with overlapping clinical features of both LQTS and CPVT |
title |
TECRL, a new life‐threatening inherited arrhythmia gene associated with overlapping clinical features of both LQTS and CPVT
|
title_full |
TECRL, a new life‐threatening inherited arrhythmia gene associated with overlapping clinical features of both LQTS and CPVT
|
title_fullStr |
TECRL, a new life‐threatening inherited arrhythmia gene associated with overlapping clinical features of both LQTS and CPVT
|
title_full_unstemmed |
TECRL, a new life‐threatening inherited arrhythmia gene associated with overlapping clinical features of both LQTS and CPVT
|
title_short |
TECRL, a new life‐threatening inherited arrhythmia gene associated with overlapping clinical features of both LQTS and CPVT
|
title_sort | tecrl, a new life‐threatening inherited arrhythmia gene associated with overlapping clinical features of both lqts and cpvt |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5167130/ https://www.ncbi.nlm.nih.gov/pubmed/27861123 http://dx.doi.org/10.15252/emmm.201505719 |
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