Performance of Kalon herpes simplex virus 2 assay using dried blood spots among young women in Uganda

This study evaluated the performance of the Kalon Biological HSV2 IgG enzyme-linked immunosorbent assay (Kalon Biological Ltd, Surrey, United Kingdom) on dried blood spots (DBS) of various dilutions compared with plasma from young women aged 18–24 years in Uganda. We estimated the sensitivity and sp...

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Detalles Bibliográficos
Autores principales: Nsobya, Sam L., Hewett, Paul C., Kalibala, Sam, Mensch, Barbara S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AOSIS 2016
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5167355/
https://www.ncbi.nlm.nih.gov/pubmed/28003966
http://dx.doi.org/10.4102/ajlm.v5i1.429
Descripción
Sumario:This study evaluated the performance of the Kalon Biological HSV2 IgG enzyme-linked immunosorbent assay (Kalon Biological Ltd, Surrey, United Kingdom) on dried blood spots (DBS) of various dilutions compared with plasma from young women aged 18–24 years in Uganda. We estimated the sensitivity and specificity of three DBS dilutions using plasma as the reference. All three evaluated DBS dilutions yielded low sensitivities and specificities, with DBS 1:2 yielding the highest concurrence. Other herpes simplex virus type 2 assays should be examined with regard to their utility for testing DBS.

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