Performance of Kalon herpes simplex virus 2 assay using dried blood spots among young women in Uganda

This study evaluated the performance of the Kalon Biological HSV2 IgG enzyme-linked immunosorbent assay (Kalon Biological Ltd, Surrey, United Kingdom) on dried blood spots (DBS) of various dilutions compared with plasma from young women aged 18–24 years in Uganda. We estimated the sensitivity and sp...

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Autores principales: Nsobya, Sam L., Hewett, Paul C., Kalibala, Sam, Mensch, Barbara S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AOSIS 2016
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5167355/
https://www.ncbi.nlm.nih.gov/pubmed/28003966
http://dx.doi.org/10.4102/ajlm.v5i1.429
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author Nsobya, Sam L.
Hewett, Paul C.
Kalibala, Sam
Mensch, Barbara S.
author_facet Nsobya, Sam L.
Hewett, Paul C.
Kalibala, Sam
Mensch, Barbara S.
author_sort Nsobya, Sam L.
collection PubMed
description This study evaluated the performance of the Kalon Biological HSV2 IgG enzyme-linked immunosorbent assay (Kalon Biological Ltd, Surrey, United Kingdom) on dried blood spots (DBS) of various dilutions compared with plasma from young women aged 18–24 years in Uganda. We estimated the sensitivity and specificity of three DBS dilutions using plasma as the reference. All three evaluated DBS dilutions yielded low sensitivities and specificities, with DBS 1:2 yielding the highest concurrence. Other herpes simplex virus type 2 assays should be examined with regard to their utility for testing DBS.
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spelling pubmed-51673552016-12-19 Performance of Kalon herpes simplex virus 2 assay using dried blood spots among young women in Uganda Nsobya, Sam L. Hewett, Paul C. Kalibala, Sam Mensch, Barbara S. Afr J Lab Med Brief Report This study evaluated the performance of the Kalon Biological HSV2 IgG enzyme-linked immunosorbent assay (Kalon Biological Ltd, Surrey, United Kingdom) on dried blood spots (DBS) of various dilutions compared with plasma from young women aged 18–24 years in Uganda. We estimated the sensitivity and specificity of three DBS dilutions using plasma as the reference. All three evaluated DBS dilutions yielded low sensitivities and specificities, with DBS 1:2 yielding the highest concurrence. Other herpes simplex virus type 2 assays should be examined with regard to their utility for testing DBS. AOSIS 2016-08-16 /pmc/articles/PMC5167355/ /pubmed/28003966 http://dx.doi.org/10.4102/ajlm.v5i1.429 Text en © 2016. The Authors http://creativecommons.org/licenses/by/2.0/ Licensee: AOSIS. This work is licensed under the Creative Commons Attribution License.
spellingShingle Brief Report
Nsobya, Sam L.
Hewett, Paul C.
Kalibala, Sam
Mensch, Barbara S.
Performance of Kalon herpes simplex virus 2 assay using dried blood spots among young women in Uganda
title Performance of Kalon herpes simplex virus 2 assay using dried blood spots among young women in Uganda
title_full Performance of Kalon herpes simplex virus 2 assay using dried blood spots among young women in Uganda
title_fullStr Performance of Kalon herpes simplex virus 2 assay using dried blood spots among young women in Uganda
title_full_unstemmed Performance of Kalon herpes simplex virus 2 assay using dried blood spots among young women in Uganda
title_short Performance of Kalon herpes simplex virus 2 assay using dried blood spots among young women in Uganda
title_sort performance of kalon herpes simplex virus 2 assay using dried blood spots among young women in uganda
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5167355/
https://www.ncbi.nlm.nih.gov/pubmed/28003966
http://dx.doi.org/10.4102/ajlm.v5i1.429
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