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The phospholipid flippase ATP9A is required for the recycling pathway from the endosomes to the plasma membrane

Type IV P-type ATPases (P4-ATPases) are phospholipid flippases that translocate phospholipids from the exoplasmic (or luminal) to the cytoplasmic leaflet of lipid bilayers. In Saccharomyces cerevisiae, P4-ATPases are localized to specific subcellular compartments and play roles in compartment-mediat...

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Autores principales: Tanaka, Yoshiki, Ono, Natsuki, Shima, Takahiro, Tanaka, Gaku, Katoh, Yohei, Nakayama, Kazuhisa, Takatsu, Hiroyuki, Shin, Hye-Won
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5170610/
https://www.ncbi.nlm.nih.gov/pubmed/27733620
http://dx.doi.org/10.1091/mbc.E16-08-0586
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author Tanaka, Yoshiki
Ono, Natsuki
Shima, Takahiro
Tanaka, Gaku
Katoh, Yohei
Nakayama, Kazuhisa
Takatsu, Hiroyuki
Shin, Hye-Won
author_facet Tanaka, Yoshiki
Ono, Natsuki
Shima, Takahiro
Tanaka, Gaku
Katoh, Yohei
Nakayama, Kazuhisa
Takatsu, Hiroyuki
Shin, Hye-Won
author_sort Tanaka, Yoshiki
collection PubMed
description Type IV P-type ATPases (P4-ATPases) are phospholipid flippases that translocate phospholipids from the exoplasmic (or luminal) to the cytoplasmic leaflet of lipid bilayers. In Saccharomyces cerevisiae, P4-ATPases are localized to specific subcellular compartments and play roles in compartment-mediated membrane trafficking; however, roles of mammalian P4-ATPases in membrane trafficking are poorly understood. We previously reported that ATP9A, one of 14 human P4-ATPases, is localized to endosomal compartments and the Golgi complex. In this study, we found that ATP9A is localized to phosphatidylserine (PS)-positive early and recycling endosomes, but not late endosomes, in HeLa cells. Depletion of ATP9A delayed the recycling of transferrin from endosomes to the plasma membrane, although it did not affect the morphology of endosomal structures. Moreover, depletion of ATP9A caused accumulation of glucose transporter 1 in endosomes, probably by inhibiting their recycling. By contrast, depletion of ATP9A affected neither the early/late endosomal transport and degradation of epidermal growth factor (EGF) nor the transport of Shiga toxin B fragment from early/recycling endosomes to the Golgi complex. Therefore ATP9A plays a crucial role in recycling from endosomes to the plasma membrane.
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spelling pubmed-51706102017-02-16 The phospholipid flippase ATP9A is required for the recycling pathway from the endosomes to the plasma membrane Tanaka, Yoshiki Ono, Natsuki Shima, Takahiro Tanaka, Gaku Katoh, Yohei Nakayama, Kazuhisa Takatsu, Hiroyuki Shin, Hye-Won Mol Biol Cell Articles Type IV P-type ATPases (P4-ATPases) are phospholipid flippases that translocate phospholipids from the exoplasmic (or luminal) to the cytoplasmic leaflet of lipid bilayers. In Saccharomyces cerevisiae, P4-ATPases are localized to specific subcellular compartments and play roles in compartment-mediated membrane trafficking; however, roles of mammalian P4-ATPases in membrane trafficking are poorly understood. We previously reported that ATP9A, one of 14 human P4-ATPases, is localized to endosomal compartments and the Golgi complex. In this study, we found that ATP9A is localized to phosphatidylserine (PS)-positive early and recycling endosomes, but not late endosomes, in HeLa cells. Depletion of ATP9A delayed the recycling of transferrin from endosomes to the plasma membrane, although it did not affect the morphology of endosomal structures. Moreover, depletion of ATP9A caused accumulation of glucose transporter 1 in endosomes, probably by inhibiting their recycling. By contrast, depletion of ATP9A affected neither the early/late endosomal transport and degradation of epidermal growth factor (EGF) nor the transport of Shiga toxin B fragment from early/recycling endosomes to the Golgi complex. Therefore ATP9A plays a crucial role in recycling from endosomes to the plasma membrane. The American Society for Cell Biology 2016-12-01 /pmc/articles/PMC5170610/ /pubmed/27733620 http://dx.doi.org/10.1091/mbc.E16-08-0586 Text en © 2016 Tanaka, Ono, et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.
spellingShingle Articles
Tanaka, Yoshiki
Ono, Natsuki
Shima, Takahiro
Tanaka, Gaku
Katoh, Yohei
Nakayama, Kazuhisa
Takatsu, Hiroyuki
Shin, Hye-Won
The phospholipid flippase ATP9A is required for the recycling pathway from the endosomes to the plasma membrane
title The phospholipid flippase ATP9A is required for the recycling pathway from the endosomes to the plasma membrane
title_full The phospholipid flippase ATP9A is required for the recycling pathway from the endosomes to the plasma membrane
title_fullStr The phospholipid flippase ATP9A is required for the recycling pathway from the endosomes to the plasma membrane
title_full_unstemmed The phospholipid flippase ATP9A is required for the recycling pathway from the endosomes to the plasma membrane
title_short The phospholipid flippase ATP9A is required for the recycling pathway from the endosomes to the plasma membrane
title_sort phospholipid flippase atp9a is required for the recycling pathway from the endosomes to the plasma membrane
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5170610/
https://www.ncbi.nlm.nih.gov/pubmed/27733620
http://dx.doi.org/10.1091/mbc.E16-08-0586
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