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The phospholipid flippase ATP9A is required for the recycling pathway from the endosomes to the plasma membrane
Type IV P-type ATPases (P4-ATPases) are phospholipid flippases that translocate phospholipids from the exoplasmic (or luminal) to the cytoplasmic leaflet of lipid bilayers. In Saccharomyces cerevisiae, P4-ATPases are localized to specific subcellular compartments and play roles in compartment-mediat...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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The American Society for Cell Biology
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5170610/ https://www.ncbi.nlm.nih.gov/pubmed/27733620 http://dx.doi.org/10.1091/mbc.E16-08-0586 |
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author | Tanaka, Yoshiki Ono, Natsuki Shima, Takahiro Tanaka, Gaku Katoh, Yohei Nakayama, Kazuhisa Takatsu, Hiroyuki Shin, Hye-Won |
author_facet | Tanaka, Yoshiki Ono, Natsuki Shima, Takahiro Tanaka, Gaku Katoh, Yohei Nakayama, Kazuhisa Takatsu, Hiroyuki Shin, Hye-Won |
author_sort | Tanaka, Yoshiki |
collection | PubMed |
description | Type IV P-type ATPases (P4-ATPases) are phospholipid flippases that translocate phospholipids from the exoplasmic (or luminal) to the cytoplasmic leaflet of lipid bilayers. In Saccharomyces cerevisiae, P4-ATPases are localized to specific subcellular compartments and play roles in compartment-mediated membrane trafficking; however, roles of mammalian P4-ATPases in membrane trafficking are poorly understood. We previously reported that ATP9A, one of 14 human P4-ATPases, is localized to endosomal compartments and the Golgi complex. In this study, we found that ATP9A is localized to phosphatidylserine (PS)-positive early and recycling endosomes, but not late endosomes, in HeLa cells. Depletion of ATP9A delayed the recycling of transferrin from endosomes to the plasma membrane, although it did not affect the morphology of endosomal structures. Moreover, depletion of ATP9A caused accumulation of glucose transporter 1 in endosomes, probably by inhibiting their recycling. By contrast, depletion of ATP9A affected neither the early/late endosomal transport and degradation of epidermal growth factor (EGF) nor the transport of Shiga toxin B fragment from early/recycling endosomes to the Golgi complex. Therefore ATP9A plays a crucial role in recycling from endosomes to the plasma membrane. |
format | Online Article Text |
id | pubmed-5170610 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-51706102017-02-16 The phospholipid flippase ATP9A is required for the recycling pathway from the endosomes to the plasma membrane Tanaka, Yoshiki Ono, Natsuki Shima, Takahiro Tanaka, Gaku Katoh, Yohei Nakayama, Kazuhisa Takatsu, Hiroyuki Shin, Hye-Won Mol Biol Cell Articles Type IV P-type ATPases (P4-ATPases) are phospholipid flippases that translocate phospholipids from the exoplasmic (or luminal) to the cytoplasmic leaflet of lipid bilayers. In Saccharomyces cerevisiae, P4-ATPases are localized to specific subcellular compartments and play roles in compartment-mediated membrane trafficking; however, roles of mammalian P4-ATPases in membrane trafficking are poorly understood. We previously reported that ATP9A, one of 14 human P4-ATPases, is localized to endosomal compartments and the Golgi complex. In this study, we found that ATP9A is localized to phosphatidylserine (PS)-positive early and recycling endosomes, but not late endosomes, in HeLa cells. Depletion of ATP9A delayed the recycling of transferrin from endosomes to the plasma membrane, although it did not affect the morphology of endosomal structures. Moreover, depletion of ATP9A caused accumulation of glucose transporter 1 in endosomes, probably by inhibiting their recycling. By contrast, depletion of ATP9A affected neither the early/late endosomal transport and degradation of epidermal growth factor (EGF) nor the transport of Shiga toxin B fragment from early/recycling endosomes to the Golgi complex. Therefore ATP9A plays a crucial role in recycling from endosomes to the plasma membrane. The American Society for Cell Biology 2016-12-01 /pmc/articles/PMC5170610/ /pubmed/27733620 http://dx.doi.org/10.1091/mbc.E16-08-0586 Text en © 2016 Tanaka, Ono, et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. |
spellingShingle | Articles Tanaka, Yoshiki Ono, Natsuki Shima, Takahiro Tanaka, Gaku Katoh, Yohei Nakayama, Kazuhisa Takatsu, Hiroyuki Shin, Hye-Won The phospholipid flippase ATP9A is required for the recycling pathway from the endosomes to the plasma membrane |
title | The phospholipid flippase ATP9A is required for the recycling pathway from the endosomes to the plasma membrane |
title_full | The phospholipid flippase ATP9A is required for the recycling pathway from the endosomes to the plasma membrane |
title_fullStr | The phospholipid flippase ATP9A is required for the recycling pathway from the endosomes to the plasma membrane |
title_full_unstemmed | The phospholipid flippase ATP9A is required for the recycling pathway from the endosomes to the plasma membrane |
title_short | The phospholipid flippase ATP9A is required for the recycling pathway from the endosomes to the plasma membrane |
title_sort | phospholipid flippase atp9a is required for the recycling pathway from the endosomes to the plasma membrane |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5170610/ https://www.ncbi.nlm.nih.gov/pubmed/27733620 http://dx.doi.org/10.1091/mbc.E16-08-0586 |
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