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Moesin and cortactin control actin-dependent multivesicular endosome biogenesis
We used in vivo and in vitro strategies to study the mechanisms of multivesicular endosome biogenesis. We found that, whereas annexinA2 and ARP2/3 mediate F-actin nucleation and branching, respectively, the ERM protein moesin supports the formation of F-actin networks on early endosomes. We also fou...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5170863/ https://www.ncbi.nlm.nih.gov/pubmed/27605702 http://dx.doi.org/10.1091/mbc.E15-12-0853 |
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author | Muriel, Olivia Tomas, Alejandra Scott, Cameron C. Gruenberg, Jean |
author_facet | Muriel, Olivia Tomas, Alejandra Scott, Cameron C. Gruenberg, Jean |
author_sort | Muriel, Olivia |
collection | PubMed |
description | We used in vivo and in vitro strategies to study the mechanisms of multivesicular endosome biogenesis. We found that, whereas annexinA2 and ARP2/3 mediate F-actin nucleation and branching, respectively, the ERM protein moesin supports the formation of F-actin networks on early endosomes. We also found that moesin plays no role during endocytosis and recycling to the plasma membrane but is absolutely required, much like actin, for early-to-late-endosome transport and multivesicular endosome formation. Both actin network formation in vitro and early-to-late endosome transport in vivo also depend on the F-actin–binding protein cortactin. Our data thus show that moesin and cortactin are necessary for formation of F-actin networks that mediate endosome biogenesis or maturation and transport through the degradative pathway. We propose that the primary function of endosomal F-actin is to control the membrane remodeling that accompanies endosome biogenesis. We also speculate that this mechanism helps segregate tubular and multivesicular membranes along the recycling and degradation pathways, respectively. |
format | Online Article Text |
id | pubmed-5170863 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-51708632017-01-16 Moesin and cortactin control actin-dependent multivesicular endosome biogenesis Muriel, Olivia Tomas, Alejandra Scott, Cameron C. Gruenberg, Jean Mol Biol Cell Articles We used in vivo and in vitro strategies to study the mechanisms of multivesicular endosome biogenesis. We found that, whereas annexinA2 and ARP2/3 mediate F-actin nucleation and branching, respectively, the ERM protein moesin supports the formation of F-actin networks on early endosomes. We also found that moesin plays no role during endocytosis and recycling to the plasma membrane but is absolutely required, much like actin, for early-to-late-endosome transport and multivesicular endosome formation. Both actin network formation in vitro and early-to-late endosome transport in vivo also depend on the F-actin–binding protein cortactin. Our data thus show that moesin and cortactin are necessary for formation of F-actin networks that mediate endosome biogenesis or maturation and transport through the degradative pathway. We propose that the primary function of endosomal F-actin is to control the membrane remodeling that accompanies endosome biogenesis. We also speculate that this mechanism helps segregate tubular and multivesicular membranes along the recycling and degradation pathways, respectively. The American Society for Cell Biology 2016-11-01 /pmc/articles/PMC5170863/ /pubmed/27605702 http://dx.doi.org/10.1091/mbc.E15-12-0853 Text en © 2016 Muriel et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. |
spellingShingle | Articles Muriel, Olivia Tomas, Alejandra Scott, Cameron C. Gruenberg, Jean Moesin and cortactin control actin-dependent multivesicular endosome biogenesis |
title | Moesin and cortactin control actin-dependent multivesicular endosome biogenesis |
title_full | Moesin and cortactin control actin-dependent multivesicular endosome biogenesis |
title_fullStr | Moesin and cortactin control actin-dependent multivesicular endosome biogenesis |
title_full_unstemmed | Moesin and cortactin control actin-dependent multivesicular endosome biogenesis |
title_short | Moesin and cortactin control actin-dependent multivesicular endosome biogenesis |
title_sort | moesin and cortactin control actin-dependent multivesicular endosome biogenesis |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5170863/ https://www.ncbi.nlm.nih.gov/pubmed/27605702 http://dx.doi.org/10.1091/mbc.E15-12-0853 |
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