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The exocytic Rabs Ypt3 and Ypt2 regulate the early step of biogenesis of the spore plasma membrane in fission yeast

During fission yeast sporulation, a membrane compartment called the forespore membrane (FSM) is newly formed on the spindle pole body (SPB). The FSM expands by membrane vesicle fusion, encapsulates the daughter nucleus resulting from meiosis, and eventually matures into the plasma membrane of the sp...

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Autores principales: Imada, Kazuki, Nakamura, Taro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5170864/
https://www.ncbi.nlm.nih.gov/pubmed/27630265
http://dx.doi.org/10.1091/mbc.E16-03-0162
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author Imada, Kazuki
Nakamura, Taro
author_facet Imada, Kazuki
Nakamura, Taro
author_sort Imada, Kazuki
collection PubMed
description During fission yeast sporulation, a membrane compartment called the forespore membrane (FSM) is newly formed on the spindle pole body (SPB). The FSM expands by membrane vesicle fusion, encapsulates the daughter nucleus resulting from meiosis, and eventually matures into the plasma membrane of the spore. Although many of the genes involved in FSM formation have been identified, its molecular mechanism is not fully understood. Here a genetic screen for sporulation-deficient mutations identified Ypt3, a Rab-family small GTPase known to function in the exocytic pathway. The ypt3-ki8 mutant showed defects in both the initiation of FSM biogenesis and FSM expansion. We also show that a mutation in Ypt2, another Rab protein that may function in the same pathway as Ypt3, compromises the initiation of FSM formation. As meiosis proceeds, both GFP-Ypt3 and GFP-Ypt2 are observed at the SPB and then relocalize to the FSM. Their localizations at the SPB precede FSM formation and depend on the meiotic SPB component Spo13, a putative GDP/GTP exchange factor for Ypt2. Given that Spo13 is essential for initiating FSM formation, these results suggest that two exocytic Rabs, Ypt3 and Ypt2, regulate the initiation of FSM formation on the SPB in concert with Spo13.
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spelling pubmed-51708642017-01-16 The exocytic Rabs Ypt3 and Ypt2 regulate the early step of biogenesis of the spore plasma membrane in fission yeast Imada, Kazuki Nakamura, Taro Mol Biol Cell Articles During fission yeast sporulation, a membrane compartment called the forespore membrane (FSM) is newly formed on the spindle pole body (SPB). The FSM expands by membrane vesicle fusion, encapsulates the daughter nucleus resulting from meiosis, and eventually matures into the plasma membrane of the spore. Although many of the genes involved in FSM formation have been identified, its molecular mechanism is not fully understood. Here a genetic screen for sporulation-deficient mutations identified Ypt3, a Rab-family small GTPase known to function in the exocytic pathway. The ypt3-ki8 mutant showed defects in both the initiation of FSM biogenesis and FSM expansion. We also show that a mutation in Ypt2, another Rab protein that may function in the same pathway as Ypt3, compromises the initiation of FSM formation. As meiosis proceeds, both GFP-Ypt3 and GFP-Ypt2 are observed at the SPB and then relocalize to the FSM. Their localizations at the SPB precede FSM formation and depend on the meiotic SPB component Spo13, a putative GDP/GTP exchange factor for Ypt2. Given that Spo13 is essential for initiating FSM formation, these results suggest that two exocytic Rabs, Ypt3 and Ypt2, regulate the initiation of FSM formation on the SPB in concert with Spo13. The American Society for Cell Biology 2016-11-01 /pmc/articles/PMC5170864/ /pubmed/27630265 http://dx.doi.org/10.1091/mbc.E16-03-0162 Text en © 2016 Imada and Nakamura. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.
spellingShingle Articles
Imada, Kazuki
Nakamura, Taro
The exocytic Rabs Ypt3 and Ypt2 regulate the early step of biogenesis of the spore plasma membrane in fission yeast
title The exocytic Rabs Ypt3 and Ypt2 regulate the early step of biogenesis of the spore plasma membrane in fission yeast
title_full The exocytic Rabs Ypt3 and Ypt2 regulate the early step of biogenesis of the spore plasma membrane in fission yeast
title_fullStr The exocytic Rabs Ypt3 and Ypt2 regulate the early step of biogenesis of the spore plasma membrane in fission yeast
title_full_unstemmed The exocytic Rabs Ypt3 and Ypt2 regulate the early step of biogenesis of the spore plasma membrane in fission yeast
title_short The exocytic Rabs Ypt3 and Ypt2 regulate the early step of biogenesis of the spore plasma membrane in fission yeast
title_sort exocytic rabs ypt3 and ypt2 regulate the early step of biogenesis of the spore plasma membrane in fission yeast
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5170864/
https://www.ncbi.nlm.nih.gov/pubmed/27630265
http://dx.doi.org/10.1091/mbc.E16-03-0162
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