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Budding yeast chromatin is dispersed in a crowded nucleoplasm in vivo

Chromatin organization has an important role in the regulation of eukaryotic systems. Although recent studies have refined the three-dimensional models of chromatin organization with high resolution at the genome sequence level, little is known about how the most fundamental units of chromatin—nucle...

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Autores principales: Chen, Chen, Lim, Hong Hwa, Shi, Jian, Tamura, Sachiko, Maeshima, Kazuhiro, Surana, Uttam, Gan, Lu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5170867/
https://www.ncbi.nlm.nih.gov/pubmed/27605704
http://dx.doi.org/10.1091/mbc.E16-07-0506
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author Chen, Chen
Lim, Hong Hwa
Shi, Jian
Tamura, Sachiko
Maeshima, Kazuhiro
Surana, Uttam
Gan, Lu
author_facet Chen, Chen
Lim, Hong Hwa
Shi, Jian
Tamura, Sachiko
Maeshima, Kazuhiro
Surana, Uttam
Gan, Lu
author_sort Chen, Chen
collection PubMed
description Chromatin organization has an important role in the regulation of eukaryotic systems. Although recent studies have refined the three-dimensional models of chromatin organization with high resolution at the genome sequence level, little is known about how the most fundamental units of chromatin—nucleosomes—are positioned in three dimensions in vivo. Here we use electron cryotomography to study chromatin organization in the budding yeast Saccharomyces cerevisiae. Direct visualization of yeast nuclear densities shows no evidence of 30-nm fibers. Aside from preribosomes and spindle microtubules, few nuclear structures are larger than a tetranucleosome. Yeast chromatin does not form compact structures in interphase or mitosis and is consistent with being in an “open” configuration that is conducive to high levels of transcription. From our study and those of others, we propose that yeast can regulate its transcription using local nucleosome–nucleosome associations.
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spelling pubmed-51708672017-01-16 Budding yeast chromatin is dispersed in a crowded nucleoplasm in vivo Chen, Chen Lim, Hong Hwa Shi, Jian Tamura, Sachiko Maeshima, Kazuhiro Surana, Uttam Gan, Lu Mol Biol Cell Articles Chromatin organization has an important role in the regulation of eukaryotic systems. Although recent studies have refined the three-dimensional models of chromatin organization with high resolution at the genome sequence level, little is known about how the most fundamental units of chromatin—nucleosomes—are positioned in three dimensions in vivo. Here we use electron cryotomography to study chromatin organization in the budding yeast Saccharomyces cerevisiae. Direct visualization of yeast nuclear densities shows no evidence of 30-nm fibers. Aside from preribosomes and spindle microtubules, few nuclear structures are larger than a tetranucleosome. Yeast chromatin does not form compact structures in interphase or mitosis and is consistent with being in an “open” configuration that is conducive to high levels of transcription. From our study and those of others, we propose that yeast can regulate its transcription using local nucleosome–nucleosome associations. The American Society for Cell Biology 2016-11-01 /pmc/articles/PMC5170867/ /pubmed/27605704 http://dx.doi.org/10.1091/mbc.E16-07-0506 Text en © 2016 Chen et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.
spellingShingle Articles
Chen, Chen
Lim, Hong Hwa
Shi, Jian
Tamura, Sachiko
Maeshima, Kazuhiro
Surana, Uttam
Gan, Lu
Budding yeast chromatin is dispersed in a crowded nucleoplasm in vivo
title Budding yeast chromatin is dispersed in a crowded nucleoplasm in vivo
title_full Budding yeast chromatin is dispersed in a crowded nucleoplasm in vivo
title_fullStr Budding yeast chromatin is dispersed in a crowded nucleoplasm in vivo
title_full_unstemmed Budding yeast chromatin is dispersed in a crowded nucleoplasm in vivo
title_short Budding yeast chromatin is dispersed in a crowded nucleoplasm in vivo
title_sort budding yeast chromatin is dispersed in a crowded nucleoplasm in vivo
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5170867/
https://www.ncbi.nlm.nih.gov/pubmed/27605704
http://dx.doi.org/10.1091/mbc.E16-07-0506
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