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Rapid dissemination of taxonomic discoveries based on DNA barcoding and morphology

The taxonomic impediment is characterized by dwindling classical taxonomic expertise, and slow pace of revisionary work, thus more rapid taxonomic assessments are needed. Here we pair rapid DNA barcoding methods with swift assessment of morphology in an effort to gauge diversity, establish species l...

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Autores principales: Cao, Xiaowei, Liu, Jie, Chen, Jian, Zheng, Guo, Kuntner, Matjaž, Agnarsson, Ingi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5171852/
https://www.ncbi.nlm.nih.gov/pubmed/27991489
http://dx.doi.org/10.1038/srep37066
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author Cao, Xiaowei
Liu, Jie
Chen, Jian
Zheng, Guo
Kuntner, Matjaž
Agnarsson, Ingi
author_facet Cao, Xiaowei
Liu, Jie
Chen, Jian
Zheng, Guo
Kuntner, Matjaž
Agnarsson, Ingi
author_sort Cao, Xiaowei
collection PubMed
description The taxonomic impediment is characterized by dwindling classical taxonomic expertise, and slow pace of revisionary work, thus more rapid taxonomic assessments are needed. Here we pair rapid DNA barcoding methods with swift assessment of morphology in an effort to gauge diversity, establish species limits, and rapidly disseminate taxonomic information prior to completion of formal taxonomic revisions. We focus on a poorly studied, but diverse spider genus, Pseudopoda, from East Asia. We augmented the standard barcoding locus (COI) with nuclear DNA sequence data (ITS2) and analyzed congruence among datasets and species delimitation methods for a total of 572 individuals representing 23 described species and many potentially new species. Our results suggest that a combination of CO1 + ITS2 fragments identify and diagnose species better than the mitochondrial barcodes alone, and that certain tree based methods yield considerably higher diversity estimates than the distance-based approaches and morphology. Combined, through an extensive field survey, we detect a twofold increase in species diversity in the surveyed area, at 42–45, with most species representing short range endemics. Our study demonstrates the power of biodiversity assessments and swift dissemination of taxonomic data through rapid inventory, and through a combination of morphological and multi-locus DNA barcoding diagnoses of diverse arthropod lineages.
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spelling pubmed-51718522016-12-28 Rapid dissemination of taxonomic discoveries based on DNA barcoding and morphology Cao, Xiaowei Liu, Jie Chen, Jian Zheng, Guo Kuntner, Matjaž Agnarsson, Ingi Sci Rep Article The taxonomic impediment is characterized by dwindling classical taxonomic expertise, and slow pace of revisionary work, thus more rapid taxonomic assessments are needed. Here we pair rapid DNA barcoding methods with swift assessment of morphology in an effort to gauge diversity, establish species limits, and rapidly disseminate taxonomic information prior to completion of formal taxonomic revisions. We focus on a poorly studied, but diverse spider genus, Pseudopoda, from East Asia. We augmented the standard barcoding locus (COI) with nuclear DNA sequence data (ITS2) and analyzed congruence among datasets and species delimitation methods for a total of 572 individuals representing 23 described species and many potentially new species. Our results suggest that a combination of CO1 + ITS2 fragments identify and diagnose species better than the mitochondrial barcodes alone, and that certain tree based methods yield considerably higher diversity estimates than the distance-based approaches and morphology. Combined, through an extensive field survey, we detect a twofold increase in species diversity in the surveyed area, at 42–45, with most species representing short range endemics. Our study demonstrates the power of biodiversity assessments and swift dissemination of taxonomic data through rapid inventory, and through a combination of morphological and multi-locus DNA barcoding diagnoses of diverse arthropod lineages. Nature Publishing Group 2016-12-19 /pmc/articles/PMC5171852/ /pubmed/27991489 http://dx.doi.org/10.1038/srep37066 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Cao, Xiaowei
Liu, Jie
Chen, Jian
Zheng, Guo
Kuntner, Matjaž
Agnarsson, Ingi
Rapid dissemination of taxonomic discoveries based on DNA barcoding and morphology
title Rapid dissemination of taxonomic discoveries based on DNA barcoding and morphology
title_full Rapid dissemination of taxonomic discoveries based on DNA barcoding and morphology
title_fullStr Rapid dissemination of taxonomic discoveries based on DNA barcoding and morphology
title_full_unstemmed Rapid dissemination of taxonomic discoveries based on DNA barcoding and morphology
title_short Rapid dissemination of taxonomic discoveries based on DNA barcoding and morphology
title_sort rapid dissemination of taxonomic discoveries based on dna barcoding and morphology
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5171852/
https://www.ncbi.nlm.nih.gov/pubmed/27991489
http://dx.doi.org/10.1038/srep37066
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