A Genome Sequence of Novel SARS-CoV Isolates: the Genotype, GD-Ins29, Leads to a Hypothesis of Viral Transmission in South China

We report a complete genomic sequence of rare isolates (minor genotype) of the SARS-CoV from SARS patients in Guangdong, China, where the first few cases emerged. The most striking discovery from the isolate is an extra 29-nucleotide sequence located at the nucleotide positions between 27,863 and 27...

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Detalles Bibliográficos
Autores principales: Qin, E’de, He, Xionglei, Tian, Wei, Liu, Yong, Li, Wei, Wen, Jie, Wang, Jingqiang, Fan, Baochang, Wu, Qingfa, Chang, Guohui, Cao, Wuchun, Xu, Zuyuan, Yang, Ruifu, Wang, Jing, Yu, Man, Li, Yan, Xu, Jing, Si, Bingyin, Hu, Yongwu, Peng, Wenming, Tang, Lin, Jiang, Tao, Shi, Jianping, Ji, Jia, Zhang, Yu, Ye, Jia, Wang, Cui’e, Han, Yujun, Zhou, Jun, Deng, Yajun, Li, Xiaoyu, Hu, Jianfei, Wang, Caiping, Yan, Chunxia, Zhang, Qingrun, Bao, Jingyue, Li, Guoqing, Chen, Weijun, Fang, Lin, Li, Changfeng, Lei, Meng, Li, Dawei, Tong, Wei, Tian, Xiangjun, Wang, Jin, Zhang, Bo, Zhang, Haiqing, Zhang, Yilin, Zhao, Hui, Zhang, Xiaowei, Li, Shuangli, Cheng, Xiaojie, Zhang, Xiuqing, Liu, Bin, Zeng, Changqing, Li, Songgang, Tan, Xuehai, Liu, Siqi, Dong, Wei, Wang, Jun, Wong, Gane Ka-Shu, Yu, Jun, Wang, Jian, Zhu, Qingyu, Yang, Huanming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2003
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5172408/
https://www.ncbi.nlm.nih.gov/pubmed/15626340
http://dx.doi.org/10.1016/S1672-0229(03)01014-3
Descripción
Sumario:We report a complete genomic sequence of rare isolates (minor genotype) of the SARS-CoV from SARS patients in Guangdong, China, where the first few cases emerged. The most striking discovery from the isolate is an extra 29-nucleotide sequence located at the nucleotide positions between 27,863 and 27,864 (referred to the complete sequence of BJ01) within an overlapped region composed of BGI-PUP5 (BGI-postulated uncharacterized protein 5) and BGI-PUP6 upstream of the N (nucleocapsid) protein. The discovery of this minor genotype, GD-Ins29, suggests a significant genetic event and differentiates it from the previously reported genotype, the dominant form among all sequenced SARS-CoV isolates. A 17-nt segment of this extra sequence is identical to a segment of the same size in two human mRNA sequences that may interfere with viral genome replication and transcription in the cytosol of the infected cells. It provides a new avenue for the exploration of the virus-host interaction in viral evolution, host pathogenesis, and vaccine development.

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