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Gene Expression Profiling in Porcine Fetal Thymus
To obtain an initial overview of gene diversity and expression pattern in porcine thymus, 11,712 ESTs (Expressed Sequence Tags) from 100-day-old porcine thymus (FTY) were sequenced and 7,071 cleaned ESTs were used for gene expression analysis. Clustered by the PHRAP program, 959 contigs and 3,074 si...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2003
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5172427/ https://www.ncbi.nlm.nih.gov/pubmed/15626347 http://dx.doi.org/10.1016/S1672-0229(03)01021-0 |
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author | Chen, Yanjiong Li, Shengbin Ye, Lin Geng, Jianing Deng, Yajun Hu, Songnian |
author_facet | Chen, Yanjiong Li, Shengbin Ye, Lin Geng, Jianing Deng, Yajun Hu, Songnian |
author_sort | Chen, Yanjiong |
collection | PubMed |
description | To obtain an initial overview of gene diversity and expression pattern in porcine thymus, 11,712 ESTs (Expressed Sequence Tags) from 100-day-old porcine thymus (FTY) were sequenced and 7,071 cleaned ESTs were used for gene expression analysis. Clustered by the PHRAP program, 959 contigs and 3,074 singlets were obtained. Blast search showed that 806 contigs and 1,669 singlets (totally 5,442 ESTs) had homologues in GenBank and 1,629 ESTs were novel. According to the Gene Ontology classification, 36.99% ESTs were cataloged into the gene expression group, indicating that although the functional gene (18.78% in defense group) of thymus is expressed in a certain degree, the 100-day-old porcine thymus still exists in a developmental stage. Comparative analysis showed that the gene expression pattern of the 100-day-old porcine thymus is similar to that of the human infant thymus. |
format | Online Article Text |
id | pubmed-5172427 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2003 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-51724272016-12-23 Gene Expression Profiling in Porcine Fetal Thymus Chen, Yanjiong Li, Shengbin Ye, Lin Geng, Jianing Deng, Yajun Hu, Songnian Genomics Proteomics Bioinformatics Brief Report To obtain an initial overview of gene diversity and expression pattern in porcine thymus, 11,712 ESTs (Expressed Sequence Tags) from 100-day-old porcine thymus (FTY) were sequenced and 7,071 cleaned ESTs were used for gene expression analysis. Clustered by the PHRAP program, 959 contigs and 3,074 singlets were obtained. Blast search showed that 806 contigs and 1,669 singlets (totally 5,442 ESTs) had homologues in GenBank and 1,629 ESTs were novel. According to the Gene Ontology classification, 36.99% ESTs were cataloged into the gene expression group, indicating that although the functional gene (18.78% in defense group) of thymus is expressed in a certain degree, the 100-day-old porcine thymus still exists in a developmental stage. Comparative analysis showed that the gene expression pattern of the 100-day-old porcine thymus is similar to that of the human infant thymus. Elsevier 2003-05 2016-11-28 /pmc/articles/PMC5172427/ /pubmed/15626347 http://dx.doi.org/10.1016/S1672-0229(03)01021-0 Text en . http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Brief Report Chen, Yanjiong Li, Shengbin Ye, Lin Geng, Jianing Deng, Yajun Hu, Songnian Gene Expression Profiling in Porcine Fetal Thymus |
title | Gene Expression Profiling in Porcine Fetal Thymus |
title_full | Gene Expression Profiling in Porcine Fetal Thymus |
title_fullStr | Gene Expression Profiling in Porcine Fetal Thymus |
title_full_unstemmed | Gene Expression Profiling in Porcine Fetal Thymus |
title_short | Gene Expression Profiling in Porcine Fetal Thymus |
title_sort | gene expression profiling in porcine fetal thymus |
topic | Brief Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5172427/ https://www.ncbi.nlm.nih.gov/pubmed/15626347 http://dx.doi.org/10.1016/S1672-0229(03)01021-0 |
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