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Preliminary Study on Detecting the SARS-CoV Specific Target cDNA Fragments by Multiplex PCR

The multiplex polymerase chain reaction (PCR) technique was applied to detect the SARS-CoV (severe acute respiratory syndrome-associated coronavirus) specific target cDNA fragments in the present study. The target cDNA fragments of SARS-CoV were synthesized artificially according to the genome seque...

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Detalles Bibliográficos
Autores principales: Chen, Wenbing, Li, Shousong, Shao, Biying, Zheng, Teng, Jiang, Shuxun, Huang, Xiaorong, Cai, Kaizhen, Zhang, Zhideng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2004
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5172431/
https://www.ncbi.nlm.nih.gov/pubmed/15629044
http://dx.doi.org/10.1016/S1672-0229(04)02008-X
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author Chen, Wenbing
Li, Shousong
Shao, Biying
Zheng, Teng
Jiang, Shuxun
Huang, Xiaorong
Cai, Kaizhen
Zhang, Zhideng
author_facet Chen, Wenbing
Li, Shousong
Shao, Biying
Zheng, Teng
Jiang, Shuxun
Huang, Xiaorong
Cai, Kaizhen
Zhang, Zhideng
author_sort Chen, Wenbing
collection PubMed
description The multiplex polymerase chain reaction (PCR) technique was applied to detect the SARS-CoV (severe acute respiratory syndrome-associated coronavirus) specific target cDNA fragments in the present study. The target cDNA fragments of SARS-CoV were synthesized artificially according to the genome sequence of SARS-CoV in GenBank submitted by The Chinese University of Hong Kong, and were used as simulated positive samples. Five primers recommended by World Health Organization (WHO) were used to amplify the fragments by single PCR and multiplex PCR. Three target cDNA fragments (121, 182 and 302 bp), as well as the three different combinations of any two of these fragments, were amplified by single PCR. The combination of these three fragments was amplified by multiplex PCR. The results indicated that the multiplex PCR technique could be applied to detect the SARS-CoV specific target cDNA fragments successfully.
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spelling pubmed-51724312016-12-23 Preliminary Study on Detecting the SARS-CoV Specific Target cDNA Fragments by Multiplex PCR Chen, Wenbing Li, Shousong Shao, Biying Zheng, Teng Jiang, Shuxun Huang, Xiaorong Cai, Kaizhen Zhang, Zhideng Genomics Proteomics Bioinformatics Brief Report The multiplex polymerase chain reaction (PCR) technique was applied to detect the SARS-CoV (severe acute respiratory syndrome-associated coronavirus) specific target cDNA fragments in the present study. The target cDNA fragments of SARS-CoV were synthesized artificially according to the genome sequence of SARS-CoV in GenBank submitted by The Chinese University of Hong Kong, and were used as simulated positive samples. Five primers recommended by World Health Organization (WHO) were used to amplify the fragments by single PCR and multiplex PCR. Three target cDNA fragments (121, 182 and 302 bp), as well as the three different combinations of any two of these fragments, were amplified by single PCR. The combination of these three fragments was amplified by multiplex PCR. The results indicated that the multiplex PCR technique could be applied to detect the SARS-CoV specific target cDNA fragments successfully. Elsevier 2004-02 2016-11-28 /pmc/articles/PMC5172431/ /pubmed/15629044 http://dx.doi.org/10.1016/S1672-0229(04)02008-X Text en . http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Brief Report
Chen, Wenbing
Li, Shousong
Shao, Biying
Zheng, Teng
Jiang, Shuxun
Huang, Xiaorong
Cai, Kaizhen
Zhang, Zhideng
Preliminary Study on Detecting the SARS-CoV Specific Target cDNA Fragments by Multiplex PCR
title Preliminary Study on Detecting the SARS-CoV Specific Target cDNA Fragments by Multiplex PCR
title_full Preliminary Study on Detecting the SARS-CoV Specific Target cDNA Fragments by Multiplex PCR
title_fullStr Preliminary Study on Detecting the SARS-CoV Specific Target cDNA Fragments by Multiplex PCR
title_full_unstemmed Preliminary Study on Detecting the SARS-CoV Specific Target cDNA Fragments by Multiplex PCR
title_short Preliminary Study on Detecting the SARS-CoV Specific Target cDNA Fragments by Multiplex PCR
title_sort preliminary study on detecting the sars-cov specific target cdna fragments by multiplex pcr
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5172431/
https://www.ncbi.nlm.nih.gov/pubmed/15629044
http://dx.doi.org/10.1016/S1672-0229(04)02008-X
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