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Post-transcriptional Gene Silencing Induced by Short Interfering RNAs in Cultured Transgenic Plant Cells

Short interfering RNA (siRNA) is widely used for studying post-transcriptional gene silencing and holds great promise as a tool for both identifying function of novel genes and validating drug targets. Two siRNA fragments (siRNA-a and -b), which were designed against different specific areas of codi...

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Autores principales: Tang, Wei, Samuels, Vanessa, Whitley, Nicki, Bloom, Nicole, DeLaGarza, Tinya, Newton, Ronald J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2004
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5172445/
https://www.ncbi.nlm.nih.gov/pubmed/15629049
http://dx.doi.org/10.1016/S1672-0229(04)02015-7
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author Tang, Wei
Samuels, Vanessa
Whitley, Nicki
Bloom, Nicole
DeLaGarza, Tinya
Newton, Ronald J.
author_facet Tang, Wei
Samuels, Vanessa
Whitley, Nicki
Bloom, Nicole
DeLaGarza, Tinya
Newton, Ronald J.
author_sort Tang, Wei
collection PubMed
description Short interfering RNA (siRNA) is widely used for studying post-transcriptional gene silencing and holds great promise as a tool for both identifying function of novel genes and validating drug targets. Two siRNA fragments (siRNA-a and -b), which were designed against different specific areas of coding region of the same target green fluorescent protein (GFP) gene, were used to silence GFP expression in cultured gfp transgenic cells of rice (Oryza sativa L.; OS), cotton (Gossypium hirsutum L.; GH), Fraser fir [Abies fraseri (Pursh) Poir; AF], and Virginia pine (Pinus virginiana Mill.; PV). Differential gene silencing was observed in the bombarded transgenic cells between two siRNAs, and these results were consistent with the inactivation of GFP confirmed by laser scanning microscopy, Northern blot, and siRNA analysis in tested transgenic cell cultures. These data suggest that siRNA-mediated gene inactivation can be the siRNA specific in different plant species. These results indicate that siRNA is a highly specific tool for targeted gene knockdown and for establishing siRNA-mediated gene silencing, which could be a reliable approach for large-scale screening of gene function and drug target validation.
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spelling pubmed-51724452016-12-23 Post-transcriptional Gene Silencing Induced by Short Interfering RNAs in Cultured Transgenic Plant Cells Tang, Wei Samuels, Vanessa Whitley, Nicki Bloom, Nicole DeLaGarza, Tinya Newton, Ronald J. Genomics Proteomics Bioinformatics Article Short interfering RNA (siRNA) is widely used for studying post-transcriptional gene silencing and holds great promise as a tool for both identifying function of novel genes and validating drug targets. Two siRNA fragments (siRNA-a and -b), which were designed against different specific areas of coding region of the same target green fluorescent protein (GFP) gene, were used to silence GFP expression in cultured gfp transgenic cells of rice (Oryza sativa L.; OS), cotton (Gossypium hirsutum L.; GH), Fraser fir [Abies fraseri (Pursh) Poir; AF], and Virginia pine (Pinus virginiana Mill.; PV). Differential gene silencing was observed in the bombarded transgenic cells between two siRNAs, and these results were consistent with the inactivation of GFP confirmed by laser scanning microscopy, Northern blot, and siRNA analysis in tested transgenic cell cultures. These data suggest that siRNA-mediated gene inactivation can be the siRNA specific in different plant species. These results indicate that siRNA is a highly specific tool for targeted gene knockdown and for establishing siRNA-mediated gene silencing, which could be a reliable approach for large-scale screening of gene function and drug target validation. Elsevier 2004-05 2016-11-28 /pmc/articles/PMC5172445/ /pubmed/15629049 http://dx.doi.org/10.1016/S1672-0229(04)02015-7 Text en . http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Tang, Wei
Samuels, Vanessa
Whitley, Nicki
Bloom, Nicole
DeLaGarza, Tinya
Newton, Ronald J.
Post-transcriptional Gene Silencing Induced by Short Interfering RNAs in Cultured Transgenic Plant Cells
title Post-transcriptional Gene Silencing Induced by Short Interfering RNAs in Cultured Transgenic Plant Cells
title_full Post-transcriptional Gene Silencing Induced by Short Interfering RNAs in Cultured Transgenic Plant Cells
title_fullStr Post-transcriptional Gene Silencing Induced by Short Interfering RNAs in Cultured Transgenic Plant Cells
title_full_unstemmed Post-transcriptional Gene Silencing Induced by Short Interfering RNAs in Cultured Transgenic Plant Cells
title_short Post-transcriptional Gene Silencing Induced by Short Interfering RNAs in Cultured Transgenic Plant Cells
title_sort post-transcriptional gene silencing induced by short interfering rnas in cultured transgenic plant cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5172445/
https://www.ncbi.nlm.nih.gov/pubmed/15629049
http://dx.doi.org/10.1016/S1672-0229(04)02015-7
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