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Proteome Analysis of Rice Root Proteins Regulated by Gibberellin
To gain an enhanced understanding of the mechanism by which gibberellins (GAs) regulate the growth and development of plants, it is necessary to identify proteins regulated by GA. Proteome analysis techniques have been applied as a direct, effective, and reliable tool in differential protein express...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2005
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5172542/ https://www.ncbi.nlm.nih.gov/pubmed/16487079 http://dx.doi.org/10.1016/S1672-0229(05)03019-6 |
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author | Komatsu, Setsuko Konishi, Hirosato |
author_facet | Komatsu, Setsuko Konishi, Hirosato |
author_sort | Komatsu, Setsuko |
collection | PubMed |
description | To gain an enhanced understanding of the mechanism by which gibberellins (GAs) regulate the growth and development of plants, it is necessary to identify proteins regulated by GA. Proteome analysis techniques have been applied as a direct, effective, and reliable tool in differential protein expressions. In previous studies, sixteen proteins showed differences in accumulation levels as a result of treatment with GA(3), uniconazole, or abscisic acid (ABA), and/or the differences between the GA-deficient semi-dwarf mutant, Tan-ginbozu, and normal cultivars. Among these proteins, aldolase increased in roots treated with GA(3), was present at low levels in Tan-ginbozu roots, and decreased in roots treated with uniconazole or ABA. In a root elongation assay, the growth of aldolase-antisense transgenic rice was half of that of vector control transgenic rice. These results indicate that increases in aldolase activity stimulate the glycolytic pathway and may play an important role in the GA-induced growth of roots. In this review, we discuss the relationship among GA, aldolase, and root growth. |
format | Online Article Text |
id | pubmed-5172542 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2005 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-51725422016-12-23 Proteome Analysis of Rice Root Proteins Regulated by Gibberellin Komatsu, Setsuko Konishi, Hirosato Genomics Proteomics Bioinformatics Review To gain an enhanced understanding of the mechanism by which gibberellins (GAs) regulate the growth and development of plants, it is necessary to identify proteins regulated by GA. Proteome analysis techniques have been applied as a direct, effective, and reliable tool in differential protein expressions. In previous studies, sixteen proteins showed differences in accumulation levels as a result of treatment with GA(3), uniconazole, or abscisic acid (ABA), and/or the differences between the GA-deficient semi-dwarf mutant, Tan-ginbozu, and normal cultivars. Among these proteins, aldolase increased in roots treated with GA(3), was present at low levels in Tan-ginbozu roots, and decreased in roots treated with uniconazole or ABA. In a root elongation assay, the growth of aldolase-antisense transgenic rice was half of that of vector control transgenic rice. These results indicate that increases in aldolase activity stimulate the glycolytic pathway and may play an important role in the GA-induced growth of roots. In this review, we discuss the relationship among GA, aldolase, and root growth. Elsevier 2005 2016-11-28 /pmc/articles/PMC5172542/ /pubmed/16487079 http://dx.doi.org/10.1016/S1672-0229(05)03019-6 Text en . http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Review Komatsu, Setsuko Konishi, Hirosato Proteome Analysis of Rice Root Proteins Regulated by Gibberellin |
title | Proteome Analysis of Rice Root Proteins Regulated by Gibberellin |
title_full | Proteome Analysis of Rice Root Proteins Regulated by Gibberellin |
title_fullStr | Proteome Analysis of Rice Root Proteins Regulated by Gibberellin |
title_full_unstemmed | Proteome Analysis of Rice Root Proteins Regulated by Gibberellin |
title_short | Proteome Analysis of Rice Root Proteins Regulated by Gibberellin |
title_sort | proteome analysis of rice root proteins regulated by gibberellin |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5172542/ https://www.ncbi.nlm.nih.gov/pubmed/16487079 http://dx.doi.org/10.1016/S1672-0229(05)03019-6 |
work_keys_str_mv | AT komatsusetsuko proteomeanalysisofricerootproteinsregulatedbygibberellin AT konishihirosato proteomeanalysisofricerootproteinsregulatedbygibberellin |