Cargando…

Proteome Analysis of Rice Root Proteins Regulated by Gibberellin

To gain an enhanced understanding of the mechanism by which gibberellins (GAs) regulate the growth and development of plants, it is necessary to identify proteins regulated by GA. Proteome analysis techniques have been applied as a direct, effective, and reliable tool in differential protein express...

Descripción completa

Detalles Bibliográficos
Autores principales: Komatsu, Setsuko, Konishi, Hirosato
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5172542/
https://www.ncbi.nlm.nih.gov/pubmed/16487079
http://dx.doi.org/10.1016/S1672-0229(05)03019-6
_version_ 1782484145554849792
author Komatsu, Setsuko
Konishi, Hirosato
author_facet Komatsu, Setsuko
Konishi, Hirosato
author_sort Komatsu, Setsuko
collection PubMed
description To gain an enhanced understanding of the mechanism by which gibberellins (GAs) regulate the growth and development of plants, it is necessary to identify proteins regulated by GA. Proteome analysis techniques have been applied as a direct, effective, and reliable tool in differential protein expressions. In previous studies, sixteen proteins showed differences in accumulation levels as a result of treatment with GA(3), uniconazole, or abscisic acid (ABA), and/or the differences between the GA-deficient semi-dwarf mutant, Tan-ginbozu, and normal cultivars. Among these proteins, aldolase increased in roots treated with GA(3), was present at low levels in Tan-ginbozu roots, and decreased in roots treated with uniconazole or ABA. In a root elongation assay, the growth of aldolase-antisense transgenic rice was half of that of vector control transgenic rice. These results indicate that increases in aldolase activity stimulate the glycolytic pathway and may play an important role in the GA-induced growth of roots. In this review, we discuss the relationship among GA, aldolase, and root growth.
format Online
Article
Text
id pubmed-5172542
institution National Center for Biotechnology Information
language English
publishDate 2005
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-51725422016-12-23 Proteome Analysis of Rice Root Proteins Regulated by Gibberellin Komatsu, Setsuko Konishi, Hirosato Genomics Proteomics Bioinformatics Review To gain an enhanced understanding of the mechanism by which gibberellins (GAs) regulate the growth and development of plants, it is necessary to identify proteins regulated by GA. Proteome analysis techniques have been applied as a direct, effective, and reliable tool in differential protein expressions. In previous studies, sixteen proteins showed differences in accumulation levels as a result of treatment with GA(3), uniconazole, or abscisic acid (ABA), and/or the differences between the GA-deficient semi-dwarf mutant, Tan-ginbozu, and normal cultivars. Among these proteins, aldolase increased in roots treated with GA(3), was present at low levels in Tan-ginbozu roots, and decreased in roots treated with uniconazole or ABA. In a root elongation assay, the growth of aldolase-antisense transgenic rice was half of that of vector control transgenic rice. These results indicate that increases in aldolase activity stimulate the glycolytic pathway and may play an important role in the GA-induced growth of roots. In this review, we discuss the relationship among GA, aldolase, and root growth. Elsevier 2005 2016-11-28 /pmc/articles/PMC5172542/ /pubmed/16487079 http://dx.doi.org/10.1016/S1672-0229(05)03019-6 Text en . http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Review
Komatsu, Setsuko
Konishi, Hirosato
Proteome Analysis of Rice Root Proteins Regulated by Gibberellin
title Proteome Analysis of Rice Root Proteins Regulated by Gibberellin
title_full Proteome Analysis of Rice Root Proteins Regulated by Gibberellin
title_fullStr Proteome Analysis of Rice Root Proteins Regulated by Gibberellin
title_full_unstemmed Proteome Analysis of Rice Root Proteins Regulated by Gibberellin
title_short Proteome Analysis of Rice Root Proteins Regulated by Gibberellin
title_sort proteome analysis of rice root proteins regulated by gibberellin
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5172542/
https://www.ncbi.nlm.nih.gov/pubmed/16487079
http://dx.doi.org/10.1016/S1672-0229(05)03019-6
work_keys_str_mv AT komatsusetsuko proteomeanalysisofricerootproteinsregulatedbygibberellin
AT konishihirosato proteomeanalysisofricerootproteinsregulatedbygibberellin