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Isolation, identification, and characterization of novel nanovesicles

Extracellular microvesicles (EVs) have been recognized for many potential clinical applications including biomarkers for disease diagnosis. In this study, we identified a major population of EVs by simply screening fluid samples with a nanosizer. Unlike other EVs, this extracellular nanovesicle (nam...

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Autores principales: Zhang, Huang-Ge, Cao, Pengxiao, Teng, Yun, Hu, Xin, Wang, Qilong, Yeri, Ashish S., Zhuang, Xiaoying, Samykutty, Abhilash, Mu, Jingyao, Deng, Zhong-Bin, Zhang, Lifeng, Mobley, James A., Yan, Jun, Van Keuren-Jensen, Kendall, Miller, Donald
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5173064/
https://www.ncbi.nlm.nih.gov/pubmed/27191656
http://dx.doi.org/10.18632/oncotarget.9325
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author Zhang, Huang-Ge
Cao, Pengxiao
Teng, Yun
Hu, Xin
Wang, Qilong
Yeri, Ashish S.
Zhuang, Xiaoying
Samykutty, Abhilash
Mu, Jingyao
Deng, Zhong-Bin
Zhang, Lifeng
Mobley, James A.
Yan, Jun
Van Keuren-Jensen, Kendall
Miller, Donald
author_facet Zhang, Huang-Ge
Cao, Pengxiao
Teng, Yun
Hu, Xin
Wang, Qilong
Yeri, Ashish S.
Zhuang, Xiaoying
Samykutty, Abhilash
Mu, Jingyao
Deng, Zhong-Bin
Zhang, Lifeng
Mobley, James A.
Yan, Jun
Van Keuren-Jensen, Kendall
Miller, Donald
author_sort Zhang, Huang-Ge
collection PubMed
description Extracellular microvesicles (EVs) have been recognized for many potential clinical applications including biomarkers for disease diagnosis. In this study, we identified a major population of EVs by simply screening fluid samples with a nanosizer. Unlike other EVs, this extracellular nanovesicle (named HG-NV, HG-NV stands for HomoGenous nanovesicle as well as for Huang-Ge- nanovesicle) can be detected with a nanosizer with minimal in vitro manipulation and are much more homogenous in size (8–12 nm) than other EVs. A simple filtration platform is capable of separating HG-NVs from peripheral blood or cell culture supernatants. In comparison with corresponding exosome profiles, HG-NVs released from both mouse and human breast tumor cells are enriched with RNAs. Tumor derived HG-NVs are more potent in promoting tumor progression than exosomes. In summary, we identified a major subset of EVs as a previously unrecognized nanovesicle. Tumor cell derived HG-NVs promote tumor progression. Molecules predominantly present in breast tumor HG-NVs have been identified and characterized. This discovery may have implications in advancing both microvesicle biology research and clinical management including potential used as a biomarker.
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spelling pubmed-51730642016-12-23 Isolation, identification, and characterization of novel nanovesicles Zhang, Huang-Ge Cao, Pengxiao Teng, Yun Hu, Xin Wang, Qilong Yeri, Ashish S. Zhuang, Xiaoying Samykutty, Abhilash Mu, Jingyao Deng, Zhong-Bin Zhang, Lifeng Mobley, James A. Yan, Jun Van Keuren-Jensen, Kendall Miller, Donald Oncotarget Research Paper Extracellular microvesicles (EVs) have been recognized for many potential clinical applications including biomarkers for disease diagnosis. In this study, we identified a major population of EVs by simply screening fluid samples with a nanosizer. Unlike other EVs, this extracellular nanovesicle (named HG-NV, HG-NV stands for HomoGenous nanovesicle as well as for Huang-Ge- nanovesicle) can be detected with a nanosizer with minimal in vitro manipulation and are much more homogenous in size (8–12 nm) than other EVs. A simple filtration platform is capable of separating HG-NVs from peripheral blood or cell culture supernatants. In comparison with corresponding exosome profiles, HG-NVs released from both mouse and human breast tumor cells are enriched with RNAs. Tumor derived HG-NVs are more potent in promoting tumor progression than exosomes. In summary, we identified a major subset of EVs as a previously unrecognized nanovesicle. Tumor cell derived HG-NVs promote tumor progression. Molecules predominantly present in breast tumor HG-NVs have been identified and characterized. This discovery may have implications in advancing both microvesicle biology research and clinical management including potential used as a biomarker. Impact Journals LLC 2016-05-12 /pmc/articles/PMC5173064/ /pubmed/27191656 http://dx.doi.org/10.18632/oncotarget.9325 Text en Copyright: © 2016 Zhang et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Zhang, Huang-Ge
Cao, Pengxiao
Teng, Yun
Hu, Xin
Wang, Qilong
Yeri, Ashish S.
Zhuang, Xiaoying
Samykutty, Abhilash
Mu, Jingyao
Deng, Zhong-Bin
Zhang, Lifeng
Mobley, James A.
Yan, Jun
Van Keuren-Jensen, Kendall
Miller, Donald
Isolation, identification, and characterization of novel nanovesicles
title Isolation, identification, and characterization of novel nanovesicles
title_full Isolation, identification, and characterization of novel nanovesicles
title_fullStr Isolation, identification, and characterization of novel nanovesicles
title_full_unstemmed Isolation, identification, and characterization of novel nanovesicles
title_short Isolation, identification, and characterization of novel nanovesicles
title_sort isolation, identification, and characterization of novel nanovesicles
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5173064/
https://www.ncbi.nlm.nih.gov/pubmed/27191656
http://dx.doi.org/10.18632/oncotarget.9325
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