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Human induced pluripotent stem cell differentiation and direct transdifferentiation into corneal epithelial-like cells
The corneal epithelium is maintained by a small pool of tissue stem cells located at the limbus. Through certain injuries or diseases this pool of stem cells may get depleted. This leads to visual impairment. Standard treatment options include autologous or allogeneic limbal stem cell (LSC) transpla...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5173137/ https://www.ncbi.nlm.nih.gov/pubmed/27275539 http://dx.doi.org/10.18632/oncotarget.9791 |
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author | Cieślar-Pobuda, Artur Rafat, Mehrdad Knoflach, Viktoria Skonieczna, Magdalena Hudecki, Andrzej Małecki, Andrzej Urasińska, Elżbieta Ghavami, Seaid Łos, Marek J. |
author_facet | Cieślar-Pobuda, Artur Rafat, Mehrdad Knoflach, Viktoria Skonieczna, Magdalena Hudecki, Andrzej Małecki, Andrzej Urasińska, Elżbieta Ghavami, Seaid Łos, Marek J. |
author_sort | Cieślar-Pobuda, Artur |
collection | PubMed |
description | The corneal epithelium is maintained by a small pool of tissue stem cells located at the limbus. Through certain injuries or diseases this pool of stem cells may get depleted. This leads to visual impairment. Standard treatment options include autologous or allogeneic limbal stem cell (LSC) transplantation, however graft rejection and chronic inflammation lowers the success rate over long time. Induced pluripotent stem (iPS) cells have opened new possibilities for treating various diseases with patient specific cells, eliminating the risk of immune rejection. In recent years, several protocols have been developed, aimed at the differentiation of iPS cells into the corneal epithelial lineage by mimicking the environmental niche of limbal stem cells. However, the risk of teratoma formation associated with the use of iPS cells hinders most applications from lab into clinics. Here we show that the differentiation of iPS cells into corneal epithelial cells results in the expression of corneal epithelial markers showing a successful differentiation, but the process is long and the level of gene expression for the pluripotency markers does not vanish completely. Therefore we set out to determine a direct transdifferentiation approach to circumvent the intermediate state of pluripotency (iPS-stage). The resulting cells, obtained by direct transdifferentiation of fibroblasts into limbal cells, exhibited corneal epithelial cell morphology and expressed corneal epithelial markers. Hence we shows for the first time a direct transdifferentiation of human dermal fibroblasts into the corneal epithelial lineage that may serve as source for corneal epithelial cells for transplantation approaches. |
format | Online Article Text |
id | pubmed-5173137 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-51731372016-12-23 Human induced pluripotent stem cell differentiation and direct transdifferentiation into corneal epithelial-like cells Cieślar-Pobuda, Artur Rafat, Mehrdad Knoflach, Viktoria Skonieczna, Magdalena Hudecki, Andrzej Małecki, Andrzej Urasińska, Elżbieta Ghavami, Seaid Łos, Marek J. Oncotarget Research Paper The corneal epithelium is maintained by a small pool of tissue stem cells located at the limbus. Through certain injuries or diseases this pool of stem cells may get depleted. This leads to visual impairment. Standard treatment options include autologous or allogeneic limbal stem cell (LSC) transplantation, however graft rejection and chronic inflammation lowers the success rate over long time. Induced pluripotent stem (iPS) cells have opened new possibilities for treating various diseases with patient specific cells, eliminating the risk of immune rejection. In recent years, several protocols have been developed, aimed at the differentiation of iPS cells into the corneal epithelial lineage by mimicking the environmental niche of limbal stem cells. However, the risk of teratoma formation associated with the use of iPS cells hinders most applications from lab into clinics. Here we show that the differentiation of iPS cells into corneal epithelial cells results in the expression of corneal epithelial markers showing a successful differentiation, but the process is long and the level of gene expression for the pluripotency markers does not vanish completely. Therefore we set out to determine a direct transdifferentiation approach to circumvent the intermediate state of pluripotency (iPS-stage). The resulting cells, obtained by direct transdifferentiation of fibroblasts into limbal cells, exhibited corneal epithelial cell morphology and expressed corneal epithelial markers. Hence we shows for the first time a direct transdifferentiation of human dermal fibroblasts into the corneal epithelial lineage that may serve as source for corneal epithelial cells for transplantation approaches. Impact Journals LLC 2016-06-02 /pmc/articles/PMC5173137/ /pubmed/27275539 http://dx.doi.org/10.18632/oncotarget.9791 Text en Copyright: © 2016 Cieślar-Pobuda et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Cieślar-Pobuda, Artur Rafat, Mehrdad Knoflach, Viktoria Skonieczna, Magdalena Hudecki, Andrzej Małecki, Andrzej Urasińska, Elżbieta Ghavami, Seaid Łos, Marek J. Human induced pluripotent stem cell differentiation and direct transdifferentiation into corneal epithelial-like cells |
title | Human induced pluripotent stem cell differentiation and direct transdifferentiation into corneal epithelial-like cells |
title_full | Human induced pluripotent stem cell differentiation and direct transdifferentiation into corneal epithelial-like cells |
title_fullStr | Human induced pluripotent stem cell differentiation and direct transdifferentiation into corneal epithelial-like cells |
title_full_unstemmed | Human induced pluripotent stem cell differentiation and direct transdifferentiation into corneal epithelial-like cells |
title_short | Human induced pluripotent stem cell differentiation and direct transdifferentiation into corneal epithelial-like cells |
title_sort | human induced pluripotent stem cell differentiation and direct transdifferentiation into corneal epithelial-like cells |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5173137/ https://www.ncbi.nlm.nih.gov/pubmed/27275539 http://dx.doi.org/10.18632/oncotarget.9791 |
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