Two-Photon Laser Scanning Stereomicroscopy for Fast Volumetric Imaging

Bessel beams have been successfully used in two-photon laser scanning fluorescence microscopy to extend the depth of field (EDF), which makes it possible to observe fast events volumetrically. However, the depth information is lost due to integration of fluorescence signals along the propagation dir...

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Detalles Bibliográficos
Autores principales: Yang, Yanlong, Yao, Baoli, Lei, Ming, Dan, Dan, Li, Runze, Horn, Mark Van, Chen, Xun, Li, Yang, Ye, Tong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5173245/
https://www.ncbi.nlm.nih.gov/pubmed/27997624
http://dx.doi.org/10.1371/journal.pone.0168885
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author Yang, Yanlong
Yao, Baoli
Lei, Ming
Dan, Dan
Li, Runze
Horn, Mark Van
Chen, Xun
Li, Yang
Ye, Tong
author_facet Yang, Yanlong
Yao, Baoli
Lei, Ming
Dan, Dan
Li, Runze
Horn, Mark Van
Chen, Xun
Li, Yang
Ye, Tong
author_sort Yang, Yanlong
collection PubMed
description Bessel beams have been successfully used in two-photon laser scanning fluorescence microscopy to extend the depth of field (EDF), which makes it possible to observe fast events volumetrically. However, the depth information is lost due to integration of fluorescence signals along the propagation direction. We describe the design and implementation of two-photon lasers scanning stereomicroscopy, which allows viewing dynamic processes in three-dimensional (3D) space stereoscopically in real-time with shutter glasses at the speed of 1.4 volumes per second. The depth information can be appreciated by human visual system or be recovered with correspondence algorithms for some cases.
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spelling pubmed-51732452017-01-04 Two-Photon Laser Scanning Stereomicroscopy for Fast Volumetric Imaging Yang, Yanlong Yao, Baoli Lei, Ming Dan, Dan Li, Runze Horn, Mark Van Chen, Xun Li, Yang Ye, Tong PLoS One Research Article Bessel beams have been successfully used in two-photon laser scanning fluorescence microscopy to extend the depth of field (EDF), which makes it possible to observe fast events volumetrically. However, the depth information is lost due to integration of fluorescence signals along the propagation direction. We describe the design and implementation of two-photon lasers scanning stereomicroscopy, which allows viewing dynamic processes in three-dimensional (3D) space stereoscopically in real-time with shutter glasses at the speed of 1.4 volumes per second. The depth information can be appreciated by human visual system or be recovered with correspondence algorithms for some cases. Public Library of Science 2016-12-20 /pmc/articles/PMC5173245/ /pubmed/27997624 http://dx.doi.org/10.1371/journal.pone.0168885 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication.
spellingShingle Research Article
Yang, Yanlong
Yao, Baoli
Lei, Ming
Dan, Dan
Li, Runze
Horn, Mark Van
Chen, Xun
Li, Yang
Ye, Tong
Two-Photon Laser Scanning Stereomicroscopy for Fast Volumetric Imaging
title Two-Photon Laser Scanning Stereomicroscopy for Fast Volumetric Imaging
title_full Two-Photon Laser Scanning Stereomicroscopy for Fast Volumetric Imaging
title_fullStr Two-Photon Laser Scanning Stereomicroscopy for Fast Volumetric Imaging
title_full_unstemmed Two-Photon Laser Scanning Stereomicroscopy for Fast Volumetric Imaging
title_short Two-Photon Laser Scanning Stereomicroscopy for Fast Volumetric Imaging
title_sort two-photon laser scanning stereomicroscopy for fast volumetric imaging
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5173245/
https://www.ncbi.nlm.nih.gov/pubmed/27997624
http://dx.doi.org/10.1371/journal.pone.0168885
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