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Annona glabra Flavonoids Act As Antimicrobials by Binding to Pseudomonas aeruginosa Cell Walls
Pseudomonas aeruginosa is an important pathogen in opportunistic infections in humans. The increased incidence of antimicrobial-resistant P. aeruginosa isolates has highlighted the need for novel and more potent therapies against this microorganism. Annona glabra is known for presenting different co...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5174114/ https://www.ncbi.nlm.nih.gov/pubmed/28066374 http://dx.doi.org/10.3389/fmicb.2016.02053 |
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author | Galvão, Stanley de S. L. Monteiro, Andrea de S. Siqueira, Ezequias P. Bomfim, Maria Rosa Q. Dias-Souza, Marcus Vinícius Ferreira, Gabriella F. Denadai, Angelo Márcio L. Santos, Áquila R. C. Lúcia dos Santos, Vera de Souza-Fagundes, Elaine M. Fernandes, Elizabeth S. Monteiro-Neto, Valério |
author_facet | Galvão, Stanley de S. L. Monteiro, Andrea de S. Siqueira, Ezequias P. Bomfim, Maria Rosa Q. Dias-Souza, Marcus Vinícius Ferreira, Gabriella F. Denadai, Angelo Márcio L. Santos, Áquila R. C. Lúcia dos Santos, Vera de Souza-Fagundes, Elaine M. Fernandes, Elizabeth S. Monteiro-Neto, Valério |
author_sort | Galvão, Stanley de S. L. |
collection | PubMed |
description | Pseudomonas aeruginosa is an important pathogen in opportunistic infections in humans. The increased incidence of antimicrobial-resistant P. aeruginosa isolates has highlighted the need for novel and more potent therapies against this microorganism. Annona glabra is known for presenting different compounds with diverse biological activities, such as anti-tumor and immunomodulatory activities. Although other species of the family display antimicrobial actions, this has not yet been reported for A. glabra. Here, we investigated the antimicrobial activity of the ethyl acetate fraction (EAF) obtained from the leaf hydroalcoholic extract of A. glabra. EAF was bactericidal against different strains of P. aeruginosa. EAF also presented with a time- and concentration-dependent effect on P. aeruginosa viability. Testing of different EAF sub-fractions showed that the sub-fraction 32-33 (SF32-33) was the most effective against P. aeruginosa. Analysis of the chemical constituents of SF32-33 demonstrated a high content of flavonoids. Incubation of this active sub-fraction with P. aeruginosa ATCC 27983 triggered an endothermic reaction, which was accompanied by an increased electric charge, suggesting a high binding of SF32-33 compounds to bacterial cell walls. Collectively, our results suggest that A. glabra-derived compounds, especially flavonoids, may be useful for treating infections caused by P. aeruginosa. |
format | Online Article Text |
id | pubmed-5174114 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-51741142017-01-06 Annona glabra Flavonoids Act As Antimicrobials by Binding to Pseudomonas aeruginosa Cell Walls Galvão, Stanley de S. L. Monteiro, Andrea de S. Siqueira, Ezequias P. Bomfim, Maria Rosa Q. Dias-Souza, Marcus Vinícius Ferreira, Gabriella F. Denadai, Angelo Márcio L. Santos, Áquila R. C. Lúcia dos Santos, Vera de Souza-Fagundes, Elaine M. Fernandes, Elizabeth S. Monteiro-Neto, Valério Front Microbiol Microbiology Pseudomonas aeruginosa is an important pathogen in opportunistic infections in humans. The increased incidence of antimicrobial-resistant P. aeruginosa isolates has highlighted the need for novel and more potent therapies against this microorganism. Annona glabra is known for presenting different compounds with diverse biological activities, such as anti-tumor and immunomodulatory activities. Although other species of the family display antimicrobial actions, this has not yet been reported for A. glabra. Here, we investigated the antimicrobial activity of the ethyl acetate fraction (EAF) obtained from the leaf hydroalcoholic extract of A. glabra. EAF was bactericidal against different strains of P. aeruginosa. EAF also presented with a time- and concentration-dependent effect on P. aeruginosa viability. Testing of different EAF sub-fractions showed that the sub-fraction 32-33 (SF32-33) was the most effective against P. aeruginosa. Analysis of the chemical constituents of SF32-33 demonstrated a high content of flavonoids. Incubation of this active sub-fraction with P. aeruginosa ATCC 27983 triggered an endothermic reaction, which was accompanied by an increased electric charge, suggesting a high binding of SF32-33 compounds to bacterial cell walls. Collectively, our results suggest that A. glabra-derived compounds, especially flavonoids, may be useful for treating infections caused by P. aeruginosa. Frontiers Media S.A. 2016-12-21 /pmc/articles/PMC5174114/ /pubmed/28066374 http://dx.doi.org/10.3389/fmicb.2016.02053 Text en Copyright © 2016 Galvão, Monteiro, Siqueira, Bomfim, Dias-Souza, Ferreira, Denadai, Santos, Santos, Souza-Fagundes, Fernandes and Monteiro-Neto. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Galvão, Stanley de S. L. Monteiro, Andrea de S. Siqueira, Ezequias P. Bomfim, Maria Rosa Q. Dias-Souza, Marcus Vinícius Ferreira, Gabriella F. Denadai, Angelo Márcio L. Santos, Áquila R. C. Lúcia dos Santos, Vera de Souza-Fagundes, Elaine M. Fernandes, Elizabeth S. Monteiro-Neto, Valério Annona glabra Flavonoids Act As Antimicrobials by Binding to Pseudomonas aeruginosa Cell Walls |
title | Annona glabra Flavonoids Act As Antimicrobials by Binding to Pseudomonas aeruginosa Cell Walls |
title_full | Annona glabra Flavonoids Act As Antimicrobials by Binding to Pseudomonas aeruginosa Cell Walls |
title_fullStr | Annona glabra Flavonoids Act As Antimicrobials by Binding to Pseudomonas aeruginosa Cell Walls |
title_full_unstemmed | Annona glabra Flavonoids Act As Antimicrobials by Binding to Pseudomonas aeruginosa Cell Walls |
title_short | Annona glabra Flavonoids Act As Antimicrobials by Binding to Pseudomonas aeruginosa Cell Walls |
title_sort | annona glabra flavonoids act as antimicrobials by binding to pseudomonas aeruginosa cell walls |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5174114/ https://www.ncbi.nlm.nih.gov/pubmed/28066374 http://dx.doi.org/10.3389/fmicb.2016.02053 |
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