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BTeam, a Novel BRET-based Biosensor for the Accurate Quantification of ATP Concentration within Living Cells
ATP levels may represent fundamental health conditions of cells. However, precise measurement of intracellular ATP levels in living cells is hindered by the lack of suitable methodologies. Here, we developed a novel ATP biosensor termed “BTeam”. BTeam comprises a yellow fluorescent protein (YFP), th...
Autores principales: | Yoshida, Tomoki, Kakizuka, Akira, Imamura, Hiromi |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5175186/ https://www.ncbi.nlm.nih.gov/pubmed/28000761 http://dx.doi.org/10.1038/srep39618 |
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