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ANGPTL2 expression in gastric cancer tissues and cells and its biological behavior
AIM: To explore expression of angiopoietin-like protein 2 (ANGPTL2) and its effect on biological behavior such as proliferation and invasiveness in gastric cancer. METHODS: Western blotting was used to detect expression of ANGPTL2 in 60 human normal gastric tissues, 60 human gastric cancer tissues a...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Baishideng Publishing Group Inc
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5175248/ https://www.ncbi.nlm.nih.gov/pubmed/28058016 http://dx.doi.org/10.3748/wjg.v22.i47.10364 |
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author | Sheng, Wei-Zhong Chen, Yu-Sheng Tu, Chuan-Tao He, Juan Zhang, Bo Gao, Wei-Dong |
author_facet | Sheng, Wei-Zhong Chen, Yu-Sheng Tu, Chuan-Tao He, Juan Zhang, Bo Gao, Wei-Dong |
author_sort | Sheng, Wei-Zhong |
collection | PubMed |
description | AIM: To explore expression of angiopoietin-like protein 2 (ANGPTL2) and its effect on biological behavior such as proliferation and invasiveness in gastric cancer. METHODS: Western blotting was used to detect expression of ANGPTL2 in 60 human normal gastric tissues, 60 human gastric cancer tissues and gastric cell lines including GES-1, N87, SGC7901, BGC823 and PAMC82. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and Transwell assay were used to detect the proliferation and invasive ability of gastric cancer cells. RESULTS: Compared to normal tissues, ANGPTL2 protein levels were significantly upregulated in gastric tissues, and this level was closely correlated with gastric tumor grade, clinical stage and lymph node metastasis. Compared to GES-1 cells, ANGPTL2 mRNA and protein levels were significantly increased in gastric cancer cells including N87, SGC7901, BGC823 and PAMC82. The expression of ANGPTL2 in highly malignant gastric cancer cell lines BGC823 and PAMC82 was significantly higher than in low malignancy gastric cancer cell lines N87 and SGC7901. MTT and Transwell experiments indicated that the proliferation rate and invasive ability of stable overexpressed gastric cancer cells was faster than in cells transfected with Lv-NC and blank control cells, and the invasive ability of stable overexpressed gastric cancer cells was higher than that of cells transfected with Lv-NC and blank control cells. CONCLUSION: ANGPTL2 contributed to proliferation and invasion of gastric cancer cells. In clinical treatment, ANGPTL2 may become a new target for treatment of gastric cancer. |
format | Online Article Text |
id | pubmed-5175248 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Baishideng Publishing Group Inc |
record_format | MEDLINE/PubMed |
spelling | pubmed-51752482017-01-05 ANGPTL2 expression in gastric cancer tissues and cells and its biological behavior Sheng, Wei-Zhong Chen, Yu-Sheng Tu, Chuan-Tao He, Juan Zhang, Bo Gao, Wei-Dong World J Gastroenterol Basic Study AIM: To explore expression of angiopoietin-like protein 2 (ANGPTL2) and its effect on biological behavior such as proliferation and invasiveness in gastric cancer. METHODS: Western blotting was used to detect expression of ANGPTL2 in 60 human normal gastric tissues, 60 human gastric cancer tissues and gastric cell lines including GES-1, N87, SGC7901, BGC823 and PAMC82. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and Transwell assay were used to detect the proliferation and invasive ability of gastric cancer cells. RESULTS: Compared to normal tissues, ANGPTL2 protein levels were significantly upregulated in gastric tissues, and this level was closely correlated with gastric tumor grade, clinical stage and lymph node metastasis. Compared to GES-1 cells, ANGPTL2 mRNA and protein levels were significantly increased in gastric cancer cells including N87, SGC7901, BGC823 and PAMC82. The expression of ANGPTL2 in highly malignant gastric cancer cell lines BGC823 and PAMC82 was significantly higher than in low malignancy gastric cancer cell lines N87 and SGC7901. MTT and Transwell experiments indicated that the proliferation rate and invasive ability of stable overexpressed gastric cancer cells was faster than in cells transfected with Lv-NC and blank control cells, and the invasive ability of stable overexpressed gastric cancer cells was higher than that of cells transfected with Lv-NC and blank control cells. CONCLUSION: ANGPTL2 contributed to proliferation and invasion of gastric cancer cells. In clinical treatment, ANGPTL2 may become a new target for treatment of gastric cancer. Baishideng Publishing Group Inc 2016-12-21 2016-12-21 /pmc/articles/PMC5175248/ /pubmed/28058016 http://dx.doi.org/10.3748/wjg.v22.i47.10364 Text en ©The Author(s) 2016. Published by Baishideng Publishing Group Inc. All rights reserved. http://creativecommons.org/licenses/by-nc/4.0/ This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. |
spellingShingle | Basic Study Sheng, Wei-Zhong Chen, Yu-Sheng Tu, Chuan-Tao He, Juan Zhang, Bo Gao, Wei-Dong ANGPTL2 expression in gastric cancer tissues and cells and its biological behavior |
title | ANGPTL2 expression in gastric cancer tissues and cells and its biological behavior |
title_full | ANGPTL2 expression in gastric cancer tissues and cells and its biological behavior |
title_fullStr | ANGPTL2 expression in gastric cancer tissues and cells and its biological behavior |
title_full_unstemmed | ANGPTL2 expression in gastric cancer tissues and cells and its biological behavior |
title_short | ANGPTL2 expression in gastric cancer tissues and cells and its biological behavior |
title_sort | angptl2 expression in gastric cancer tissues and cells and its biological behavior |
topic | Basic Study |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5175248/ https://www.ncbi.nlm.nih.gov/pubmed/28058016 http://dx.doi.org/10.3748/wjg.v22.i47.10364 |
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