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A programmable Cas9-serine recombinase fusion protein that operates on DNA sequences in mammalian cells

We describe the development of ‘recCas9’, an RNA-programmed small serine recombinase that functions in mammalian cells. We fused a catalytically inactive dCas9 to the catalytic domain of Gin recombinase using an optimized fusion architecture. The resulting recCas9 system recombines DNA sites contain...

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Detalles Bibliográficos
Autores principales:	Chaikind, Brian, Bessen, Jeffrey L., Thompson, David B., Hu, Johnny H., Liu, David R.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2016
Materias:	Genome Integrity, Repair and Replication
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5175349/ https://www.ncbi.nlm.nih.gov/pubmed/27515511 http://dx.doi.org/10.1093/nar/gkw707

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