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Short-term Exposure to 50-Hz Electromagnetic Field and Alterations in NQO1 and NQO2 Expression in MCF-7 Cells

AIM: Extremely low-frequency electromagnetic fields (ELF-EMFs) have some genotoxic effects and it may alter the mRNA levels of antioxidant genes. The NAD(P)H: quinone oxidoreductase-1 (NQO1) and NQO2 are ubiquitously expressed. Considering that there is no published data on the effect(s) of ELF-EMF...

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Autores principales: Mahmoudinasab, Hamideh, Saadat, Mostafa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Institute of Immunobiology and Human Genetics 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5175497/
https://www.ncbi.nlm.nih.gov/pubmed/28028389
http://dx.doi.org/10.3889/oamjms.2016.102
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author Mahmoudinasab, Hamideh
Saadat, Mostafa
author_facet Mahmoudinasab, Hamideh
Saadat, Mostafa
author_sort Mahmoudinasab, Hamideh
collection PubMed
description AIM: Extremely low-frequency electromagnetic fields (ELF-EMFs) have some genotoxic effects and it may alter the mRNA levels of antioxidant genes. The NAD(P)H: quinone oxidoreductase-1 (NQO1) and NQO2 are ubiquitously expressed. Considering that there is no published data on the effect(s) of ELF-EMF (50-Hz) exposure and expression levels of NQO1 and NQO2 in the human MCF-7 cells, the present study was carried out. METHODS: The ELF-EMF (0.25 and 0.50 mT) exposure patterns were: 5 min field-on/5 min filed-off, 15 min field-on/15 min field-off, and 30 min field-on continuously. In all exposure conditions, total exposure time were 30 minutes. The RNA extraction was done at two times; immediately post exposure and two hours post exposure. The effect of ELF-EMF on gene expression was assessed by real-time PCR. RESULTS: The NQO1 mRNA level (at 0h) decreased in the cells exposed to 5 min field-on/5 min filed-off condition at 0.25 mT EMF when compared with the unexposed cells. The NQO2 mRNA level (at 0h and 2h) increased in the cells exposed to 5 min field-on/5 min filed-off condition at 0.50 mT EMF when compared with the unexposed cells. CONCLUSIONS: Alterations in the NQO1 and NQO2 mRNA levels seem at the “5 min field-on/5 min field-off” condition.
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spelling pubmed-51754972016-12-27 Short-term Exposure to 50-Hz Electromagnetic Field and Alterations in NQO1 and NQO2 Expression in MCF-7 Cells Mahmoudinasab, Hamideh Saadat, Mostafa Open Access Maced J Med Sci Basic Science AIM: Extremely low-frequency electromagnetic fields (ELF-EMFs) have some genotoxic effects and it may alter the mRNA levels of antioxidant genes. The NAD(P)H: quinone oxidoreductase-1 (NQO1) and NQO2 are ubiquitously expressed. Considering that there is no published data on the effect(s) of ELF-EMF (50-Hz) exposure and expression levels of NQO1 and NQO2 in the human MCF-7 cells, the present study was carried out. METHODS: The ELF-EMF (0.25 and 0.50 mT) exposure patterns were: 5 min field-on/5 min filed-off, 15 min field-on/15 min field-off, and 30 min field-on continuously. In all exposure conditions, total exposure time were 30 minutes. The RNA extraction was done at two times; immediately post exposure and two hours post exposure. The effect of ELF-EMF on gene expression was assessed by real-time PCR. RESULTS: The NQO1 mRNA level (at 0h) decreased in the cells exposed to 5 min field-on/5 min filed-off condition at 0.25 mT EMF when compared with the unexposed cells. The NQO2 mRNA level (at 0h and 2h) increased in the cells exposed to 5 min field-on/5 min filed-off condition at 0.50 mT EMF when compared with the unexposed cells. CONCLUSIONS: Alterations in the NQO1 and NQO2 mRNA levels seem at the “5 min field-on/5 min field-off” condition. Institute of Immunobiology and Human Genetics 2016-12-15 2016-09-24 /pmc/articles/PMC5175497/ /pubmed/28028389 http://dx.doi.org/10.3889/oamjms.2016.102 Text en Copyright: © 2016 Hamideh Mahmoudinasab, Mostafa Saadat. http://creativecommons.org/licenses/CC BY-NC/4.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0).
spellingShingle Basic Science
Mahmoudinasab, Hamideh
Saadat, Mostafa
Short-term Exposure to 50-Hz Electromagnetic Field and Alterations in NQO1 and NQO2 Expression in MCF-7 Cells
title Short-term Exposure to 50-Hz Electromagnetic Field and Alterations in NQO1 and NQO2 Expression in MCF-7 Cells
title_full Short-term Exposure to 50-Hz Electromagnetic Field and Alterations in NQO1 and NQO2 Expression in MCF-7 Cells
title_fullStr Short-term Exposure to 50-Hz Electromagnetic Field and Alterations in NQO1 and NQO2 Expression in MCF-7 Cells
title_full_unstemmed Short-term Exposure to 50-Hz Electromagnetic Field and Alterations in NQO1 and NQO2 Expression in MCF-7 Cells
title_short Short-term Exposure to 50-Hz Electromagnetic Field and Alterations in NQO1 and NQO2 Expression in MCF-7 Cells
title_sort short-term exposure to 50-hz electromagnetic field and alterations in nqo1 and nqo2 expression in mcf-7 cells
topic Basic Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5175497/
https://www.ncbi.nlm.nih.gov/pubmed/28028389
http://dx.doi.org/10.3889/oamjms.2016.102
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