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Potassium Aspartate Attenuates Brain Injury Induced by Controlled Cortical Impact in Rats Through Increasing Adenosine Triphosphate (ATP) Levels, Na(+)/K(+)-ATPase Activity and Reducing Brain Edema

BACKGROUND: Potassium aspartate (PA), as an electrolyte supplement, is widely used in clinical practice. In our previous study, we found PA had neuroprotective effects against apoptosis after cerebral ischemia/reperfusion in rats. In this study, we examine whether PA has protective effects on trauma...

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Autores principales: Gu, Yi, Zhang, Jie, Zhao, Yumei, Su, Yujin, Zhang, Yazhuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Scientific Literature, Inc. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5175720/
https://www.ncbi.nlm.nih.gov/pubmed/27959885
http://dx.doi.org/10.12659/MSM.898185
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author Gu, Yi
Zhang, Jie
Zhao, Yumei
Su, Yujin
Zhang, Yazhuo
author_facet Gu, Yi
Zhang, Jie
Zhao, Yumei
Su, Yujin
Zhang, Yazhuo
author_sort Gu, Yi
collection PubMed
description BACKGROUND: Potassium aspartate (PA), as an electrolyte supplement, is widely used in clinical practice. In our previous study, we found PA had neuroprotective effects against apoptosis after cerebral ischemia/reperfusion in rats. In this study, we examine whether PA has protective effects on traumatic brain injury (TBI). MATERIAL/METHODS: TBI was induced by controlled cortical impact (CCI) in rats. Vehicle treatment (control) or PA treatment was administered intraperitoneally at 30 minutes after CCI. The modified neurological severity score (mNSS) and cortical lesion volume were examined. Brain edema and blood-brain barrier (BBB) integrity were measured, as well as brain ATP contents, lactic acid levels, and Na(+)/K(+)-ATPase activities. RESULTS: We found that CCI induced cortical injury in rats. Acute PA treatment at the dose of 62.5 mg/kg and 125 mg/kg significantly improved neurological deficits (p<0.05 and p<0.001, respectively) and decreased the cortical lesion volume (p<0.05 and p<0.001, respectively) compared with vehicle-only treatment. PA treatment at the dose of 125 mg/kg attenuated brain edema and ameliorated BBB integrity. In addition, PA treatment significantly reduced the loss of ATP (p<0.01), reduced lactic acid levels (p<0.001), and increased the activity of Na(+)/K(+)-ATPase (p<0.01). CONCLUSIONS: Our results indicate PA has neuroprotective effects on TBI through increasing ATP levels, Na(+)/K(+)-ATPase activity, and reducing brain edema. It provides experimental evidence for the clinical application of PA.
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spelling pubmed-51757202017-01-03 Potassium Aspartate Attenuates Brain Injury Induced by Controlled Cortical Impact in Rats Through Increasing Adenosine Triphosphate (ATP) Levels, Na(+)/K(+)-ATPase Activity and Reducing Brain Edema Gu, Yi Zhang, Jie Zhao, Yumei Su, Yujin Zhang, Yazhuo Med Sci Monit Animal Study BACKGROUND: Potassium aspartate (PA), as an electrolyte supplement, is widely used in clinical practice. In our previous study, we found PA had neuroprotective effects against apoptosis after cerebral ischemia/reperfusion in rats. In this study, we examine whether PA has protective effects on traumatic brain injury (TBI). MATERIAL/METHODS: TBI was induced by controlled cortical impact (CCI) in rats. Vehicle treatment (control) or PA treatment was administered intraperitoneally at 30 minutes after CCI. The modified neurological severity score (mNSS) and cortical lesion volume were examined. Brain edema and blood-brain barrier (BBB) integrity were measured, as well as brain ATP contents, lactic acid levels, and Na(+)/K(+)-ATPase activities. RESULTS: We found that CCI induced cortical injury in rats. Acute PA treatment at the dose of 62.5 mg/kg and 125 mg/kg significantly improved neurological deficits (p<0.05 and p<0.001, respectively) and decreased the cortical lesion volume (p<0.05 and p<0.001, respectively) compared with vehicle-only treatment. PA treatment at the dose of 125 mg/kg attenuated brain edema and ameliorated BBB integrity. In addition, PA treatment significantly reduced the loss of ATP (p<0.01), reduced lactic acid levels (p<0.001), and increased the activity of Na(+)/K(+)-ATPase (p<0.01). CONCLUSIONS: Our results indicate PA has neuroprotective effects on TBI through increasing ATP levels, Na(+)/K(+)-ATPase activity, and reducing brain edema. It provides experimental evidence for the clinical application of PA. International Scientific Literature, Inc. 2016-12-13 /pmc/articles/PMC5175720/ /pubmed/27959885 http://dx.doi.org/10.12659/MSM.898185 Text en © Med Sci Monit, 2016 This work is licensed under Creative Common Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
spellingShingle Animal Study
Gu, Yi
Zhang, Jie
Zhao, Yumei
Su, Yujin
Zhang, Yazhuo
Potassium Aspartate Attenuates Brain Injury Induced by Controlled Cortical Impact in Rats Through Increasing Adenosine Triphosphate (ATP) Levels, Na(+)/K(+)-ATPase Activity and Reducing Brain Edema
title Potassium Aspartate Attenuates Brain Injury Induced by Controlled Cortical Impact in Rats Through Increasing Adenosine Triphosphate (ATP) Levels, Na(+)/K(+)-ATPase Activity and Reducing Brain Edema
title_full Potassium Aspartate Attenuates Brain Injury Induced by Controlled Cortical Impact in Rats Through Increasing Adenosine Triphosphate (ATP) Levels, Na(+)/K(+)-ATPase Activity and Reducing Brain Edema
title_fullStr Potassium Aspartate Attenuates Brain Injury Induced by Controlled Cortical Impact in Rats Through Increasing Adenosine Triphosphate (ATP) Levels, Na(+)/K(+)-ATPase Activity and Reducing Brain Edema
title_full_unstemmed Potassium Aspartate Attenuates Brain Injury Induced by Controlled Cortical Impact in Rats Through Increasing Adenosine Triphosphate (ATP) Levels, Na(+)/K(+)-ATPase Activity and Reducing Brain Edema
title_short Potassium Aspartate Attenuates Brain Injury Induced by Controlled Cortical Impact in Rats Through Increasing Adenosine Triphosphate (ATP) Levels, Na(+)/K(+)-ATPase Activity and Reducing Brain Edema
title_sort potassium aspartate attenuates brain injury induced by controlled cortical impact in rats through increasing adenosine triphosphate (atp) levels, na(+)/k(+)-atpase activity and reducing brain edema
topic Animal Study
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5175720/
https://www.ncbi.nlm.nih.gov/pubmed/27959885
http://dx.doi.org/10.12659/MSM.898185
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