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Influence of Polymorphisms in the HTR3A and HTR3B Genes on Experimental Pain and the Effect of the 5-HT(3) Antagonist Granisetron
The aim of this study was to investigate experimentally if 5-HT(3) single nucleotide polymorphisms (SNP) contribute to pain perception and efficacy of the 5-HT(3)-antagonist granisetron and sex differences. Sixty healthy participants were genotyped regarding HTR3A (rs1062613) and HTR3B (rs1176744)....
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5176308/ https://www.ncbi.nlm.nih.gov/pubmed/28002447 http://dx.doi.org/10.1371/journal.pone.0168703 |
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author | Louca Jounger, Sofia Christidis, Nikolaos Hedenberg-Magnusson, Britt List, Thomas Svensson, Peter Schalling, Martin Ernberg, Malin |
author_facet | Louca Jounger, Sofia Christidis, Nikolaos Hedenberg-Magnusson, Britt List, Thomas Svensson, Peter Schalling, Martin Ernberg, Malin |
author_sort | Louca Jounger, Sofia |
collection | PubMed |
description | The aim of this study was to investigate experimentally if 5-HT(3) single nucleotide polymorphisms (SNP) contribute to pain perception and efficacy of the 5-HT(3)-antagonist granisetron and sex differences. Sixty healthy participants were genotyped regarding HTR3A (rs1062613) and HTR3B (rs1176744). First, pain was induced by bilateral hypertonic saline injections (HS, 5.5%, 0.2 mL) into the masseter muscles. Thirty min later the masseter muscle on one side was pretreated with 0.5 mL granisetron (1 mg/mL) and on the other side with 0.5 mL placebo (isotonic saline) followed by another HS injection (0.2 mL). Pain intensity, pain duration, pain area and pressure pain thresholds (PPTs) were assessed after each injection. HS evoked moderate pain, with higher intensity in the women (P = 0.023), but had no effect on PPTs. None of the SNPs influenced any pain variable in general, but compared to men, the pain area was larger in women carrying the C/C (HTR3A) (P = 0.015) and pain intensity higher in women with the A/C alleles (HTR3B) (P = 0.019). Pre-treatment with granisetron reduced pain intensity, duration and area to a lesser degree in women (P < 0.05), but the SNPs did not in general influence the efficacy of granisetron. Women carrying the C/T & T/T (HTR3A) genotype had less reduction of pain intensity (P = 0.041) and area (P = 0.005), and women with the C/C genotype (HTR3B) had less reduction of pain intensity (P = 0.030), duration (P = 0.030) and area compared to men (P = 0.017). In conclusion, SNPs did not influence experimental muscle pain or the effect of granisetron on pain variables in general, but there were some sex differences in pain variables that seem to be influenced by genotypes. However, due to the small sample size further research is needed before any firm conclusions can be drawn. |
format | Online Article Text |
id | pubmed-5176308 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-51763082017-01-04 Influence of Polymorphisms in the HTR3A and HTR3B Genes on Experimental Pain and the Effect of the 5-HT(3) Antagonist Granisetron Louca Jounger, Sofia Christidis, Nikolaos Hedenberg-Magnusson, Britt List, Thomas Svensson, Peter Schalling, Martin Ernberg, Malin PLoS One Research Article The aim of this study was to investigate experimentally if 5-HT(3) single nucleotide polymorphisms (SNP) contribute to pain perception and efficacy of the 5-HT(3)-antagonist granisetron and sex differences. Sixty healthy participants were genotyped regarding HTR3A (rs1062613) and HTR3B (rs1176744). First, pain was induced by bilateral hypertonic saline injections (HS, 5.5%, 0.2 mL) into the masseter muscles. Thirty min later the masseter muscle on one side was pretreated with 0.5 mL granisetron (1 mg/mL) and on the other side with 0.5 mL placebo (isotonic saline) followed by another HS injection (0.2 mL). Pain intensity, pain duration, pain area and pressure pain thresholds (PPTs) were assessed after each injection. HS evoked moderate pain, with higher intensity in the women (P = 0.023), but had no effect on PPTs. None of the SNPs influenced any pain variable in general, but compared to men, the pain area was larger in women carrying the C/C (HTR3A) (P = 0.015) and pain intensity higher in women with the A/C alleles (HTR3B) (P = 0.019). Pre-treatment with granisetron reduced pain intensity, duration and area to a lesser degree in women (P < 0.05), but the SNPs did not in general influence the efficacy of granisetron. Women carrying the C/T & T/T (HTR3A) genotype had less reduction of pain intensity (P = 0.041) and area (P = 0.005), and women with the C/C genotype (HTR3B) had less reduction of pain intensity (P = 0.030), duration (P = 0.030) and area compared to men (P = 0.017). In conclusion, SNPs did not influence experimental muscle pain or the effect of granisetron on pain variables in general, but there were some sex differences in pain variables that seem to be influenced by genotypes. However, due to the small sample size further research is needed before any firm conclusions can be drawn. Public Library of Science 2016-12-21 /pmc/articles/PMC5176308/ /pubmed/28002447 http://dx.doi.org/10.1371/journal.pone.0168703 Text en © 2016 Louca Jounger et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Louca Jounger, Sofia Christidis, Nikolaos Hedenberg-Magnusson, Britt List, Thomas Svensson, Peter Schalling, Martin Ernberg, Malin Influence of Polymorphisms in the HTR3A and HTR3B Genes on Experimental Pain and the Effect of the 5-HT(3) Antagonist Granisetron |
title | Influence of Polymorphisms in the HTR3A and HTR3B Genes on Experimental Pain and the Effect of the 5-HT(3) Antagonist Granisetron |
title_full | Influence of Polymorphisms in the HTR3A and HTR3B Genes on Experimental Pain and the Effect of the 5-HT(3) Antagonist Granisetron |
title_fullStr | Influence of Polymorphisms in the HTR3A and HTR3B Genes on Experimental Pain and the Effect of the 5-HT(3) Antagonist Granisetron |
title_full_unstemmed | Influence of Polymorphisms in the HTR3A and HTR3B Genes on Experimental Pain and the Effect of the 5-HT(3) Antagonist Granisetron |
title_short | Influence of Polymorphisms in the HTR3A and HTR3B Genes on Experimental Pain and the Effect of the 5-HT(3) Antagonist Granisetron |
title_sort | influence of polymorphisms in the htr3a and htr3b genes on experimental pain and the effect of the 5-ht(3) antagonist granisetron |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5176308/ https://www.ncbi.nlm.nih.gov/pubmed/28002447 http://dx.doi.org/10.1371/journal.pone.0168703 |
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