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Dynamics of Bovine Sperm Interaction with Epithelium Differ Between Oviductal Isthmus and Ampulla

In mammals, many sperm that reach the oviduct are held in a reservoir by binding to epithelium. To leave the reservoir, sperm detach from the epithelium; however, they may bind and detach again as they ascend into the ampulla toward oocytes. In order to elucidate the nature of binding interactions a...

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Autores principales: Ardon, Florencia, Markello, Ross D., Hu, Lian, Deutsch, Zarah I., Tung, Chih-Kuan, Wu, Mingming, Suarez, Susan S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Society for the Study of Reproduction, Inc. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5176364/
https://www.ncbi.nlm.nih.gov/pubmed/27605344
http://dx.doi.org/10.1095/biolreprod.116.140632
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author Ardon, Florencia
Markello, Ross D.
Hu, Lian
Deutsch, Zarah I.
Tung, Chih-Kuan
Wu, Mingming
Suarez, Susan S.
author_facet Ardon, Florencia
Markello, Ross D.
Hu, Lian
Deutsch, Zarah I.
Tung, Chih-Kuan
Wu, Mingming
Suarez, Susan S.
author_sort Ardon, Florencia
collection PubMed
description In mammals, many sperm that reach the oviduct are held in a reservoir by binding to epithelium. To leave the reservoir, sperm detach from the epithelium; however, they may bind and detach again as they ascend into the ampulla toward oocytes. In order to elucidate the nature of binding interactions along the oviduct, we compared the effects of bursts of strong fluid flow (as would be caused by oviductal contractions), heparin, and hyperactivation on detachment of bovine sperm bound in vitro to epithelium on intact folds of isthmic and ampullar mucosa. Intact folds of oviductal mucosa were used to represent the strong attachments of epithelial cells to each other and to underlying connective tissue that exist in vivo. Effects of heparin on binding were tested because heparin binds to the Binder of SPerm (BSP) proteins that attach sperm to oviductal epithelium. Sperm bound by their heads to beating cilia on both isthmic and ampullar epithelia and could not be detached by strong bursts of fluid flow. Addition of heparin immediately detached sperm from isthmic epithelium but not ampullar epithelium. Addition of 4-aminopyridine immediately stimulated hyperactivation of sperm but did not detach them from isthmic or ampullar epithelium unless added with heparin. These observations indicate that the nature of binding of sperm to ampullar epithelium differs from that of binding to isthmic epithelium; specifically, sperm bound to isthmic epithelium can be detached by heparin alone, while sperm bound to ampullar epithelium requires both heparin and hyperactivation to detach from the epithelium.
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spelling pubmed-51763642017-10-01 Dynamics of Bovine Sperm Interaction with Epithelium Differ Between Oviductal Isthmus and Ampulla Ardon, Florencia Markello, Ross D. Hu, Lian Deutsch, Zarah I. Tung, Chih-Kuan Wu, Mingming Suarez, Susan S. Biol Reprod Articles In mammals, many sperm that reach the oviduct are held in a reservoir by binding to epithelium. To leave the reservoir, sperm detach from the epithelium; however, they may bind and detach again as they ascend into the ampulla toward oocytes. In order to elucidate the nature of binding interactions along the oviduct, we compared the effects of bursts of strong fluid flow (as would be caused by oviductal contractions), heparin, and hyperactivation on detachment of bovine sperm bound in vitro to epithelium on intact folds of isthmic and ampullar mucosa. Intact folds of oviductal mucosa were used to represent the strong attachments of epithelial cells to each other and to underlying connective tissue that exist in vivo. Effects of heparin on binding were tested because heparin binds to the Binder of SPerm (BSP) proteins that attach sperm to oviductal epithelium. Sperm bound by their heads to beating cilia on both isthmic and ampullar epithelia and could not be detached by strong bursts of fluid flow. Addition of heparin immediately detached sperm from isthmic epithelium but not ampullar epithelium. Addition of 4-aminopyridine immediately stimulated hyperactivation of sperm but did not detach them from isthmic or ampullar epithelium unless added with heparin. These observations indicate that the nature of binding of sperm to ampullar epithelium differs from that of binding to isthmic epithelium; specifically, sperm bound to isthmic epithelium can be detached by heparin alone, while sperm bound to ampullar epithelium requires both heparin and hyperactivation to detach from the epithelium. Society for the Study of Reproduction, Inc. 2016-09-07 2016-10 /pmc/articles/PMC5176364/ /pubmed/27605344 http://dx.doi.org/10.1095/biolreprod.116.140632 Text en © 2016 by the Society for the Study of Reproduction, Inc. http://creativecommons.org/licenses/by-nc/4.0/ This article is available under a Creative Commons License 4.0 (Attribution-Non-Commercial), as described at http://creativecommons.org/licenses/by-nc/4.0
spellingShingle Articles
Ardon, Florencia
Markello, Ross D.
Hu, Lian
Deutsch, Zarah I.
Tung, Chih-Kuan
Wu, Mingming
Suarez, Susan S.
Dynamics of Bovine Sperm Interaction with Epithelium Differ Between Oviductal Isthmus and Ampulla
title Dynamics of Bovine Sperm Interaction with Epithelium Differ Between Oviductal Isthmus and Ampulla
title_full Dynamics of Bovine Sperm Interaction with Epithelium Differ Between Oviductal Isthmus and Ampulla
title_fullStr Dynamics of Bovine Sperm Interaction with Epithelium Differ Between Oviductal Isthmus and Ampulla
title_full_unstemmed Dynamics of Bovine Sperm Interaction with Epithelium Differ Between Oviductal Isthmus and Ampulla
title_short Dynamics of Bovine Sperm Interaction with Epithelium Differ Between Oviductal Isthmus and Ampulla
title_sort dynamics of bovine sperm interaction with epithelium differ between oviductal isthmus and ampulla
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5176364/
https://www.ncbi.nlm.nih.gov/pubmed/27605344
http://dx.doi.org/10.1095/biolreprod.116.140632
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