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Combination of density gradient centrifugation and swim-up methods effectively decreases morphologically abnormal sperms

Density gradient centrifugation (DGC) and swim-up techniques have been reported for semen preparation in assisted reproductive techniques in humans. We investigated whether semen preparation using a combination of DGC and swim-up techniques could effectively decrease morphologically abnormal human s...

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Autores principales: YAMANAKA, Masaya, TOMITA, Kazuhisa, HASHIMOTO, Shu, MATSUMOTO, Hiroshi, SATOH, Manabu, KATO, Hiromi, HOSOI, Yoshihiko, INOUE, Masayasu, NAKAOKA, Yoshiharu, MORIMOTO, Yoshiharu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Society for Reproduction and Development 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5177978/
https://www.ncbi.nlm.nih.gov/pubmed/27616283
http://dx.doi.org/10.1262/jrd.2016-112
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author YAMANAKA, Masaya
TOMITA, Kazuhisa
HASHIMOTO, Shu
MATSUMOTO, Hiroshi
SATOH, Manabu
KATO, Hiromi
HOSOI, Yoshihiko
INOUE, Masayasu
NAKAOKA, Yoshiharu
MORIMOTO, Yoshiharu
author_facet YAMANAKA, Masaya
TOMITA, Kazuhisa
HASHIMOTO, Shu
MATSUMOTO, Hiroshi
SATOH, Manabu
KATO, Hiromi
HOSOI, Yoshihiko
INOUE, Masayasu
NAKAOKA, Yoshiharu
MORIMOTO, Yoshiharu
author_sort YAMANAKA, Masaya
collection PubMed
description Density gradient centrifugation (DGC) and swim-up techniques have been reported for semen preparation in assisted reproductive techniques in humans. We investigated whether semen preparation using a combination of DGC and swim-up techniques could effectively decrease morphologically abnormal human sperms at the ultrastructural level. Semen samples were obtained from 16 infertile males and fractionated by swim-up following DGC. Ultrastructural abnormalities of sperms obtained from original semen, lower layer of swim-up following DGC, and upper layer of swim-up following DGC were analyzed by transmission electron microscopy. The correlation among ultrastructural head abnormality in sperms from the upper layer of swim-up, fertilization in in vitro fertilization, and pregnancy after embryo transfer was also investigated. Furthermore, sperms with DNA fragmentation in the samples processed via a combination of DGC and swim-up was assessed in a sperm chromatin structure assay. Ultrastructural abnormalities in sperm heads and tails in the upper layer after swim-up following DGC was the lowest among the three groups. Sperms with nuclear vacuoles were the most difficult to eliminate using a combination of DGC and swim-up in all types of head abnormalities. A negative correlation was confirmed between the fertilization rates of intracytoplasmic sperm injection and head abnormality of sperms obtained from the upper layer of the swim-up following DGC. Sperms with DNA fragmentation were effectively decreased using the combination of two techniques. In conclusion, the combination of DGC and swim-up effectively decreased the number of sperms with ultrastructural abnormalities both in the head and in the tail. However, sperms with ultrastructural abnormalities that cannot be completely decreased using a combination of DGC and swim-up may impair fertilization in some cases of intracytoplasmic sperm injection.
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spelling pubmed-51779782016-12-23 Combination of density gradient centrifugation and swim-up methods effectively decreases morphologically abnormal sperms YAMANAKA, Masaya TOMITA, Kazuhisa HASHIMOTO, Shu MATSUMOTO, Hiroshi SATOH, Manabu KATO, Hiromi HOSOI, Yoshihiko INOUE, Masayasu NAKAOKA, Yoshiharu MORIMOTO, Yoshiharu J Reprod Dev Original Article Density gradient centrifugation (DGC) and swim-up techniques have been reported for semen preparation in assisted reproductive techniques in humans. We investigated whether semen preparation using a combination of DGC and swim-up techniques could effectively decrease morphologically abnormal human sperms at the ultrastructural level. Semen samples were obtained from 16 infertile males and fractionated by swim-up following DGC. Ultrastructural abnormalities of sperms obtained from original semen, lower layer of swim-up following DGC, and upper layer of swim-up following DGC were analyzed by transmission electron microscopy. The correlation among ultrastructural head abnormality in sperms from the upper layer of swim-up, fertilization in in vitro fertilization, and pregnancy after embryo transfer was also investigated. Furthermore, sperms with DNA fragmentation in the samples processed via a combination of DGC and swim-up was assessed in a sperm chromatin structure assay. Ultrastructural abnormalities in sperm heads and tails in the upper layer after swim-up following DGC was the lowest among the three groups. Sperms with nuclear vacuoles were the most difficult to eliminate using a combination of DGC and swim-up in all types of head abnormalities. A negative correlation was confirmed between the fertilization rates of intracytoplasmic sperm injection and head abnormality of sperms obtained from the upper layer of the swim-up following DGC. Sperms with DNA fragmentation were effectively decreased using the combination of two techniques. In conclusion, the combination of DGC and swim-up effectively decreased the number of sperms with ultrastructural abnormalities both in the head and in the tail. However, sperms with ultrastructural abnormalities that cannot be completely decreased using a combination of DGC and swim-up may impair fertilization in some cases of intracytoplasmic sperm injection. The Society for Reproduction and Development 2016-09-11 2016-12 /pmc/articles/PMC5177978/ /pubmed/27616283 http://dx.doi.org/10.1262/jrd.2016-112 Text en ©2016 Society for Reproduction and Development http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Original Article
YAMANAKA, Masaya
TOMITA, Kazuhisa
HASHIMOTO, Shu
MATSUMOTO, Hiroshi
SATOH, Manabu
KATO, Hiromi
HOSOI, Yoshihiko
INOUE, Masayasu
NAKAOKA, Yoshiharu
MORIMOTO, Yoshiharu
Combination of density gradient centrifugation and swim-up methods effectively decreases morphologically abnormal sperms
title Combination of density gradient centrifugation and swim-up methods effectively decreases morphologically abnormal sperms
title_full Combination of density gradient centrifugation and swim-up methods effectively decreases morphologically abnormal sperms
title_fullStr Combination of density gradient centrifugation and swim-up methods effectively decreases morphologically abnormal sperms
title_full_unstemmed Combination of density gradient centrifugation and swim-up methods effectively decreases morphologically abnormal sperms
title_short Combination of density gradient centrifugation and swim-up methods effectively decreases morphologically abnormal sperms
title_sort combination of density gradient centrifugation and swim-up methods effectively decreases morphologically abnormal sperms
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5177978/
https://www.ncbi.nlm.nih.gov/pubmed/27616283
http://dx.doi.org/10.1262/jrd.2016-112
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