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PATHFAST, a novel chemiluminescent enzyme immunoassay for measuring estradiol in equine whole blood and serum
A novel chemiluminescent enzyme immunoassay system, PATHFAST, for the measurement of estradiol in horses was evaluated. The concentrations of estradiol in the whole blood and serum of mares were measured using PATHFAST and the estradiol concentrations measured by PATHFAST were compared with those me...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Society for Reproduction and Development
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5177982/ https://www.ncbi.nlm.nih.gov/pubmed/27545960 http://dx.doi.org/10.1262/jrd.2016-038 |
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author | TOISHI, Yuko TSUNODA, Nobuo KUME, Kouiti NAGAOKA, Kentaro WATANABE, Gen TAYA, Kazuyoshi |
author_facet | TOISHI, Yuko TSUNODA, Nobuo KUME, Kouiti NAGAOKA, Kentaro WATANABE, Gen TAYA, Kazuyoshi |
author_sort | TOISHI, Yuko |
collection | PubMed |
description | A novel chemiluminescent enzyme immunoassay system, PATHFAST, for the measurement of estradiol in horses was evaluated. The concentrations of estradiol in the whole blood and serum of mares were measured using PATHFAST and the estradiol concentrations measured by PATHFAST were compared with those measured by a time-resolved fluoro-immunoassay (FIA). To monitor physiological changes, serum estradiol concentrations in mares were measured using PATHFAST throughout the gestation period. The serum estradiol concentrations correlated highly with those in whole blood samples. The serum concentrations of estradiol measured by PATHFAST also correlated well with FIA. Circulating estradiol increased during mid-gestation and high levels of serum estradiol were maintained in late gestation, followed by an abrupt decline to term. These results demonstrate the utility of PATHFAST in equine clinics as an accurate diagnostic tool for the rapid assay of estradiol within 26 min using unextracted whole blood. |
format | Online Article Text |
id | pubmed-5177982 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | The Society for Reproduction and Development |
record_format | MEDLINE/PubMed |
spelling | pubmed-51779822016-12-23 PATHFAST, a novel chemiluminescent enzyme immunoassay for measuring estradiol in equine whole blood and serum TOISHI, Yuko TSUNODA, Nobuo KUME, Kouiti NAGAOKA, Kentaro WATANABE, Gen TAYA, Kazuyoshi J Reprod Dev Technology Report A novel chemiluminescent enzyme immunoassay system, PATHFAST, for the measurement of estradiol in horses was evaluated. The concentrations of estradiol in the whole blood and serum of mares were measured using PATHFAST and the estradiol concentrations measured by PATHFAST were compared with those measured by a time-resolved fluoro-immunoassay (FIA). To monitor physiological changes, serum estradiol concentrations in mares were measured using PATHFAST throughout the gestation period. The serum estradiol concentrations correlated highly with those in whole blood samples. The serum concentrations of estradiol measured by PATHFAST also correlated well with FIA. Circulating estradiol increased during mid-gestation and high levels of serum estradiol were maintained in late gestation, followed by an abrupt decline to term. These results demonstrate the utility of PATHFAST in equine clinics as an accurate diagnostic tool for the rapid assay of estradiol within 26 min using unextracted whole blood. The Society for Reproduction and Development 2016-08-20 2016-12 /pmc/articles/PMC5177982/ /pubmed/27545960 http://dx.doi.org/10.1262/jrd.2016-038 Text en ©2016 Society for Reproduction and Development http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. |
spellingShingle | Technology Report TOISHI, Yuko TSUNODA, Nobuo KUME, Kouiti NAGAOKA, Kentaro WATANABE, Gen TAYA, Kazuyoshi PATHFAST, a novel chemiluminescent enzyme immunoassay for measuring estradiol in equine whole blood and serum |
title | PATHFAST, a novel chemiluminescent enzyme immunoassay for measuring estradiol in equine whole blood and serum |
title_full | PATHFAST, a novel chemiluminescent enzyme immunoassay for measuring estradiol in equine whole blood and serum |
title_fullStr | PATHFAST, a novel chemiluminescent enzyme immunoassay for measuring estradiol in equine whole blood and serum |
title_full_unstemmed | PATHFAST, a novel chemiluminescent enzyme immunoassay for measuring estradiol in equine whole blood and serum |
title_short | PATHFAST, a novel chemiluminescent enzyme immunoassay for measuring estradiol in equine whole blood and serum |
title_sort | pathfast, a novel chemiluminescent enzyme immunoassay for measuring estradiol in equine whole blood and serum |
topic | Technology Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5177982/ https://www.ncbi.nlm.nih.gov/pubmed/27545960 http://dx.doi.org/10.1262/jrd.2016-038 |
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