Cargando…

PATHFAST, a novel chemiluminescent enzyme immunoassay for measuring estradiol in equine whole blood and serum

A novel chemiluminescent enzyme immunoassay system, PATHFAST, for the measurement of estradiol in horses was evaluated. The concentrations of estradiol in the whole blood and serum of mares were measured using PATHFAST and the estradiol concentrations measured by PATHFAST were compared with those me...

Descripción completa

Detalles Bibliográficos
Autores principales: TOISHI, Yuko, TSUNODA, Nobuo, KUME, Kouiti, NAGAOKA, Kentaro, WATANABE, Gen, TAYA, Kazuyoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Society for Reproduction and Development 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5177982/
https://www.ncbi.nlm.nih.gov/pubmed/27545960
http://dx.doi.org/10.1262/jrd.2016-038
_version_ 1782485093746475008
author TOISHI, Yuko
TSUNODA, Nobuo
KUME, Kouiti
NAGAOKA, Kentaro
WATANABE, Gen
TAYA, Kazuyoshi
author_facet TOISHI, Yuko
TSUNODA, Nobuo
KUME, Kouiti
NAGAOKA, Kentaro
WATANABE, Gen
TAYA, Kazuyoshi
author_sort TOISHI, Yuko
collection PubMed
description A novel chemiluminescent enzyme immunoassay system, PATHFAST, for the measurement of estradiol in horses was evaluated. The concentrations of estradiol in the whole blood and serum of mares were measured using PATHFAST and the estradiol concentrations measured by PATHFAST were compared with those measured by a time-resolved fluoro-immunoassay (FIA). To monitor physiological changes, serum estradiol concentrations in mares were measured using PATHFAST throughout the gestation period. The serum estradiol concentrations correlated highly with those in whole blood samples. The serum concentrations of estradiol measured by PATHFAST also correlated well with FIA. Circulating estradiol increased during mid-gestation and high levels of serum estradiol were maintained in late gestation, followed by an abrupt decline to term. These results demonstrate the utility of PATHFAST in equine clinics as an accurate diagnostic tool for the rapid assay of estradiol within 26 min using unextracted whole blood.
format Online
Article
Text
id pubmed-5177982
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher The Society for Reproduction and Development
record_format MEDLINE/PubMed
spelling pubmed-51779822016-12-23 PATHFAST, a novel chemiluminescent enzyme immunoassay for measuring estradiol in equine whole blood and serum TOISHI, Yuko TSUNODA, Nobuo KUME, Kouiti NAGAOKA, Kentaro WATANABE, Gen TAYA, Kazuyoshi J Reprod Dev Technology Report A novel chemiluminescent enzyme immunoassay system, PATHFAST, for the measurement of estradiol in horses was evaluated. The concentrations of estradiol in the whole blood and serum of mares were measured using PATHFAST and the estradiol concentrations measured by PATHFAST were compared with those measured by a time-resolved fluoro-immunoassay (FIA). To monitor physiological changes, serum estradiol concentrations in mares were measured using PATHFAST throughout the gestation period. The serum estradiol concentrations correlated highly with those in whole blood samples. The serum concentrations of estradiol measured by PATHFAST also correlated well with FIA. Circulating estradiol increased during mid-gestation and high levels of serum estradiol were maintained in late gestation, followed by an abrupt decline to term. These results demonstrate the utility of PATHFAST in equine clinics as an accurate diagnostic tool for the rapid assay of estradiol within 26 min using unextracted whole blood. The Society for Reproduction and Development 2016-08-20 2016-12 /pmc/articles/PMC5177982/ /pubmed/27545960 http://dx.doi.org/10.1262/jrd.2016-038 Text en ©2016 Society for Reproduction and Development http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Technology Report
TOISHI, Yuko
TSUNODA, Nobuo
KUME, Kouiti
NAGAOKA, Kentaro
WATANABE, Gen
TAYA, Kazuyoshi
PATHFAST, a novel chemiluminescent enzyme immunoassay for measuring estradiol in equine whole blood and serum
title PATHFAST, a novel chemiluminescent enzyme immunoassay for measuring estradiol in equine whole blood and serum
title_full PATHFAST, a novel chemiluminescent enzyme immunoassay for measuring estradiol in equine whole blood and serum
title_fullStr PATHFAST, a novel chemiluminescent enzyme immunoassay for measuring estradiol in equine whole blood and serum
title_full_unstemmed PATHFAST, a novel chemiluminescent enzyme immunoassay for measuring estradiol in equine whole blood and serum
title_short PATHFAST, a novel chemiluminescent enzyme immunoassay for measuring estradiol in equine whole blood and serum
title_sort pathfast, a novel chemiluminescent enzyme immunoassay for measuring estradiol in equine whole blood and serum
topic Technology Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5177982/
https://www.ncbi.nlm.nih.gov/pubmed/27545960
http://dx.doi.org/10.1262/jrd.2016-038
work_keys_str_mv AT toishiyuko pathfastanovelchemiluminescentenzymeimmunoassayformeasuringestradiolinequinewholebloodandserum
AT tsunodanobuo pathfastanovelchemiluminescentenzymeimmunoassayformeasuringestradiolinequinewholebloodandserum
AT kumekouiti pathfastanovelchemiluminescentenzymeimmunoassayformeasuringestradiolinequinewholebloodandserum
AT nagaokakentaro pathfastanovelchemiluminescentenzymeimmunoassayformeasuringestradiolinequinewholebloodandserum
AT watanabegen pathfastanovelchemiluminescentenzymeimmunoassayformeasuringestradiolinequinewholebloodandserum
AT tayakazuyoshi pathfastanovelchemiluminescentenzymeimmunoassayformeasuringestradiolinequinewholebloodandserum