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Efficient pig ICSI using Percoll-selected spermatozoa; evidence for the essential role of phospholipase C-ζ in ICSI success

In pigs, the damaged sperm membrane leads to leakage of phospholipase C-ζ (PLCζ), which has been identified as a sperm factor, and a reduction of oocyte-activating ability. In this study, we investigated whether sperm selected by Percoll gradient centrifugation (Percoll) have sufficient PLCζ, and wh...

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Detalles Bibliográficos
Autores principales: NAKAI, Michiko, SUZUKI, Shun-ichi, ITO, Junya, FUCHIMOTO, Dai-ichiro, SEMBON, Shoichiro, NOGUCHI, Junko, ONISHI, Akira, KASHIWAZAKI, Naomi, KIKUCHI, Kazuhiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Society for Reproduction and Development 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5177984/
https://www.ncbi.nlm.nih.gov/pubmed/27725346
http://dx.doi.org/10.1262/jrd.2016-103