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Efficient pig ICSI using Percoll-selected spermatozoa; evidence for the essential role of phospholipase C-ζ in ICSI success
In pigs, the damaged sperm membrane leads to leakage of phospholipase C-ζ (PLCζ), which has been identified as a sperm factor, and a reduction of oocyte-activating ability. In this study, we investigated whether sperm selected by Percoll gradient centrifugation (Percoll) have sufficient PLCζ, and wh...
Autores principales: | NAKAI, Michiko, SUZUKI, Shun-ichi, ITO, Junya, FUCHIMOTO, Dai-ichiro, SEMBON, Shoichiro, NOGUCHI, Junko, ONISHI, Akira, KASHIWAZAKI, Naomi, KIKUCHI, Kazuhiro |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Society for Reproduction and Development
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5177984/ https://www.ncbi.nlm.nih.gov/pubmed/27725346 http://dx.doi.org/10.1262/jrd.2016-103 |
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