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Consumption of antimicrobial manuka honey does not significantly perturb the microbiota in the hind gut of mice

The aim of this study was to test the hypothesis that consuming manuka honey, which contains antimicrobial methylglyoxal, may affect the gut microbiota. We undertook a mouse feeding study to investigate whether dietary manuka honey supplementation altered microbial numbers and their production of or...

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Autores principales: Rosendale, Doug, Butts, Christine A., de Guzman, Cloe Erika, Maddox, Ian S., Martell, Sheridan, McIntyre, Lynn, Skinner, Margot A., Dinnan, Hannah, Ansell, Juliet
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5178337/
https://www.ncbi.nlm.nih.gov/pubmed/28028466
http://dx.doi.org/10.7717/peerj.2787
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author Rosendale, Doug
Butts, Christine A.
de Guzman, Cloe Erika
Maddox, Ian S.
Martell, Sheridan
McIntyre, Lynn
Skinner, Margot A.
Dinnan, Hannah
Ansell, Juliet
author_facet Rosendale, Doug
Butts, Christine A.
de Guzman, Cloe Erika
Maddox, Ian S.
Martell, Sheridan
McIntyre, Lynn
Skinner, Margot A.
Dinnan, Hannah
Ansell, Juliet
author_sort Rosendale, Doug
collection PubMed
description The aim of this study was to test the hypothesis that consuming manuka honey, which contains antimicrobial methylglyoxal, may affect the gut microbiota. We undertook a mouse feeding study to investigate whether dietary manuka honey supplementation altered microbial numbers and their production of organic acid products from carbohydrate fermentation, which are markers of gut microbiota function. The caecum of C57BL/6 mice fed a diet supplemented with antimicrobial UMF® 20+ manuka honey at 2.2 g/kg animal did not show any significantly changed concentrations of microbial short chain fatty acids as measured by gas chromatography, except for increased formate and lowered succinate organic acid concentrations, compared to mice fed a control diet. There was no change in succinate-producing Bacteroidetes numbers, or honey-utilising Bifidobacteria, nor any other microbes measured by real time quantitative PCR. These results suggest that, despite the antimicrobial activity of the original honey, consumption of manuka honey only mildly affects substrate metabolism by the gut microbiota.
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spelling pubmed-51783372016-12-27 Consumption of antimicrobial manuka honey does not significantly perturb the microbiota in the hind gut of mice Rosendale, Doug Butts, Christine A. de Guzman, Cloe Erika Maddox, Ian S. Martell, Sheridan McIntyre, Lynn Skinner, Margot A. Dinnan, Hannah Ansell, Juliet PeerJ Biochemistry The aim of this study was to test the hypothesis that consuming manuka honey, which contains antimicrobial methylglyoxal, may affect the gut microbiota. We undertook a mouse feeding study to investigate whether dietary manuka honey supplementation altered microbial numbers and their production of organic acid products from carbohydrate fermentation, which are markers of gut microbiota function. The caecum of C57BL/6 mice fed a diet supplemented with antimicrobial UMF® 20+ manuka honey at 2.2 g/kg animal did not show any significantly changed concentrations of microbial short chain fatty acids as measured by gas chromatography, except for increased formate and lowered succinate organic acid concentrations, compared to mice fed a control diet. There was no change in succinate-producing Bacteroidetes numbers, or honey-utilising Bifidobacteria, nor any other microbes measured by real time quantitative PCR. These results suggest that, despite the antimicrobial activity of the original honey, consumption of manuka honey only mildly affects substrate metabolism by the gut microbiota. PeerJ Inc. 2016-12-20 /pmc/articles/PMC5178337/ /pubmed/28028466 http://dx.doi.org/10.7717/peerj.2787 Text en ©2016 Rosendale et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Biochemistry
Rosendale, Doug
Butts, Christine A.
de Guzman, Cloe Erika
Maddox, Ian S.
Martell, Sheridan
McIntyre, Lynn
Skinner, Margot A.
Dinnan, Hannah
Ansell, Juliet
Consumption of antimicrobial manuka honey does not significantly perturb the microbiota in the hind gut of mice
title Consumption of antimicrobial manuka honey does not significantly perturb the microbiota in the hind gut of mice
title_full Consumption of antimicrobial manuka honey does not significantly perturb the microbiota in the hind gut of mice
title_fullStr Consumption of antimicrobial manuka honey does not significantly perturb the microbiota in the hind gut of mice
title_full_unstemmed Consumption of antimicrobial manuka honey does not significantly perturb the microbiota in the hind gut of mice
title_short Consumption of antimicrobial manuka honey does not significantly perturb the microbiota in the hind gut of mice
title_sort consumption of antimicrobial manuka honey does not significantly perturb the microbiota in the hind gut of mice
topic Biochemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5178337/
https://www.ncbi.nlm.nih.gov/pubmed/28028466
http://dx.doi.org/10.7717/peerj.2787
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