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Antiproliferative and Pro-Apoptotic Effects of MiR-4286 Inhibition in Melanoma Cells
INTRODUCTION: MicroRNAs are essential regulators of gene expression at the post-transcriptional level. Their expression is altered in cancer tissues, and evaluation of these alterations is considered a promising tool used to diagnose and identify prognostic markers. MATERIALS AND METHODS: The microR...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5179095/ https://www.ncbi.nlm.nih.gov/pubmed/28005927 http://dx.doi.org/10.1371/journal.pone.0168229 |
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author | Komina, Anna Palkina, Nadezhda Aksenenko, Mariya Tsyrenzhapova, Seseg Ruksha, Tatiana |
author_facet | Komina, Anna Palkina, Nadezhda Aksenenko, Mariya Tsyrenzhapova, Seseg Ruksha, Tatiana |
author_sort | Komina, Anna |
collection | PubMed |
description | INTRODUCTION: MicroRNAs are essential regulators of gene expression at the post-transcriptional level. Their expression is altered in cancer tissues, and evaluation of these alterations is considered a promising tool used to diagnose and identify prognostic markers. MATERIALS AND METHODS: The microRNA expression profiles of formalin-fixed, paraffin-embedded melanoma and melanocytic nevi samples were estimated with a microarray and subsequently validated by real-time PCR. Melanoma cells were transfected with miR-4286 inhibitor to evaluate the influence of this microRNA on the viability, proliferation, apoptosis, migration, and invasion of melanoma cells. RESULTS: The microarray revealed that the expression of 1,171 microRNAs was altered in melanoma samples compared to melanocytic nevi. Real-time PCR validation experiments found the microRNA expression levels to correspond to the melanoma/melanocytic nevi microarray results. The pathway analysis identified 52 modulated pathways in melanoma. Moreover, the application of miR-4286 inhibitor to BRO melanoma cells resulted in a 2.6-fold increase in the apoptosis rate and a 1.7-fold decrease in the cell proliferation/viability but did not affect the invasiveness and migration of these cells. Furthermore, the use of miR-4286 inhibitor altered the mRNA expression of several miR-4286 gene targets: folylpolyglutamate synthase, RNA polymerase I-specific transcription initiation factor, apelin, G-protein-coupled receptor 55, and high-mobility group A1 protein, which have been implicated in cell proliferation/apoptosis regulation. Lastly, the transiently transfected SK-MEL-1 cells with miR-4286 inhibitor decreased proliferation rate and modulated folylpolyglutamate synthase rates of these cells. CONCLUSION: Our results demonstrate that miR-4286 mediates proliferation and apoptosis in melanoma cells, these findings may represent a novel mechanism underlying these processes. |
format | Online Article Text |
id | pubmed-5179095 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-51790952017-01-04 Antiproliferative and Pro-Apoptotic Effects of MiR-4286 Inhibition in Melanoma Cells Komina, Anna Palkina, Nadezhda Aksenenko, Mariya Tsyrenzhapova, Seseg Ruksha, Tatiana PLoS One Research Article INTRODUCTION: MicroRNAs are essential regulators of gene expression at the post-transcriptional level. Their expression is altered in cancer tissues, and evaluation of these alterations is considered a promising tool used to diagnose and identify prognostic markers. MATERIALS AND METHODS: The microRNA expression profiles of formalin-fixed, paraffin-embedded melanoma and melanocytic nevi samples were estimated with a microarray and subsequently validated by real-time PCR. Melanoma cells were transfected with miR-4286 inhibitor to evaluate the influence of this microRNA on the viability, proliferation, apoptosis, migration, and invasion of melanoma cells. RESULTS: The microarray revealed that the expression of 1,171 microRNAs was altered in melanoma samples compared to melanocytic nevi. Real-time PCR validation experiments found the microRNA expression levels to correspond to the melanoma/melanocytic nevi microarray results. The pathway analysis identified 52 modulated pathways in melanoma. Moreover, the application of miR-4286 inhibitor to BRO melanoma cells resulted in a 2.6-fold increase in the apoptosis rate and a 1.7-fold decrease in the cell proliferation/viability but did not affect the invasiveness and migration of these cells. Furthermore, the use of miR-4286 inhibitor altered the mRNA expression of several miR-4286 gene targets: folylpolyglutamate synthase, RNA polymerase I-specific transcription initiation factor, apelin, G-protein-coupled receptor 55, and high-mobility group A1 protein, which have been implicated in cell proliferation/apoptosis regulation. Lastly, the transiently transfected SK-MEL-1 cells with miR-4286 inhibitor decreased proliferation rate and modulated folylpolyglutamate synthase rates of these cells. CONCLUSION: Our results demonstrate that miR-4286 mediates proliferation and apoptosis in melanoma cells, these findings may represent a novel mechanism underlying these processes. Public Library of Science 2016-12-22 /pmc/articles/PMC5179095/ /pubmed/28005927 http://dx.doi.org/10.1371/journal.pone.0168229 Text en © 2016 Komina et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Komina, Anna Palkina, Nadezhda Aksenenko, Mariya Tsyrenzhapova, Seseg Ruksha, Tatiana Antiproliferative and Pro-Apoptotic Effects of MiR-4286 Inhibition in Melanoma Cells |
title | Antiproliferative and Pro-Apoptotic Effects of MiR-4286 Inhibition in Melanoma Cells |
title_full | Antiproliferative and Pro-Apoptotic Effects of MiR-4286 Inhibition in Melanoma Cells |
title_fullStr | Antiproliferative and Pro-Apoptotic Effects of MiR-4286 Inhibition in Melanoma Cells |
title_full_unstemmed | Antiproliferative and Pro-Apoptotic Effects of MiR-4286 Inhibition in Melanoma Cells |
title_short | Antiproliferative and Pro-Apoptotic Effects of MiR-4286 Inhibition in Melanoma Cells |
title_sort | antiproliferative and pro-apoptotic effects of mir-4286 inhibition in melanoma cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5179095/ https://www.ncbi.nlm.nih.gov/pubmed/28005927 http://dx.doi.org/10.1371/journal.pone.0168229 |
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