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A novel method of cultivating cardiac myocytes in agarose microchamber chips for studying cell synchronization

We have developed a new method that enables agar microstructures to be used to cultivate cardiac myocyte cells in a manner that allows their connection patterns to be controlled. Non-contact three-dimensional photo-thermal etching with a 1064-nm infrared focused laser beam was used to form the shape...

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Detalles Bibliográficos
Autores principales: Kojima, Kensuke, Kaneko, Tomoyuki, Yasuda, Kenji
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2004
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC517946/
https://www.ncbi.nlm.nih.gov/pubmed/15357869
http://dx.doi.org/10.1186/1477-3155-2-9
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author Kojima, Kensuke
Kaneko, Tomoyuki
Yasuda, Kenji
author_facet Kojima, Kensuke
Kaneko, Tomoyuki
Yasuda, Kenji
author_sort Kojima, Kensuke
collection PubMed
description We have developed a new method that enables agar microstructures to be used to cultivate cardiac myocyte cells in a manner that allows their connection patterns to be controlled. Non-contact three-dimensional photo-thermal etching with a 1064-nm infrared focused laser beam was used to form the shapes of agar microstructures. This wavelength was selected as it is not absorbed by water or agar. Identical rat cardiac myocytes were cultured in adjacent microstructures connected by microchannels and the interactions of asynchronous beating cardiac myocyte cells observed. Two isolated and independently beating cardiac myocytes were shown to form contacts through the narrow microchannels and by 90 minutes had synchronized their oscillations. This occurred by one of the two cells stopping their oscillation and following the pattern of the other cell. In contrast, when two sets of synchronized beating cells came into contact, those two sets synchronized without any observable interruptions to their rhythms. The results indicate that the synchronization process of cardiac myocytes may be dependent on the community size and network pattern of these cells.
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spelling pubmed-5179462004-09-24 A novel method of cultivating cardiac myocytes in agarose microchamber chips for studying cell synchronization Kojima, Kensuke Kaneko, Tomoyuki Yasuda, Kenji J Nanobiotechnology Short Communication We have developed a new method that enables agar microstructures to be used to cultivate cardiac myocyte cells in a manner that allows their connection patterns to be controlled. Non-contact three-dimensional photo-thermal etching with a 1064-nm infrared focused laser beam was used to form the shapes of agar microstructures. This wavelength was selected as it is not absorbed by water or agar. Identical rat cardiac myocytes were cultured in adjacent microstructures connected by microchannels and the interactions of asynchronous beating cardiac myocyte cells observed. Two isolated and independently beating cardiac myocytes were shown to form contacts through the narrow microchannels and by 90 minutes had synchronized their oscillations. This occurred by one of the two cells stopping their oscillation and following the pattern of the other cell. In contrast, when two sets of synchronized beating cells came into contact, those two sets synchronized without any observable interruptions to their rhythms. The results indicate that the synchronization process of cardiac myocytes may be dependent on the community size and network pattern of these cells. BioMed Central 2004-09-09 /pmc/articles/PMC517946/ /pubmed/15357869 http://dx.doi.org/10.1186/1477-3155-2-9 Text en Copyright © 2004 Kojima et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open-access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Kojima, Kensuke
Kaneko, Tomoyuki
Yasuda, Kenji
A novel method of cultivating cardiac myocytes in agarose microchamber chips for studying cell synchronization
title A novel method of cultivating cardiac myocytes in agarose microchamber chips for studying cell synchronization
title_full A novel method of cultivating cardiac myocytes in agarose microchamber chips for studying cell synchronization
title_fullStr A novel method of cultivating cardiac myocytes in agarose microchamber chips for studying cell synchronization
title_full_unstemmed A novel method of cultivating cardiac myocytes in agarose microchamber chips for studying cell synchronization
title_short A novel method of cultivating cardiac myocytes in agarose microchamber chips for studying cell synchronization
title_sort novel method of cultivating cardiac myocytes in agarose microchamber chips for studying cell synchronization
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC517946/
https://www.ncbi.nlm.nih.gov/pubmed/15357869
http://dx.doi.org/10.1186/1477-3155-2-9
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