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Optimisation of the two-dimensional gel electrophoresis protocol using the Taguchi approach

BACKGROUND: Quantitative proteomic analyses have traditionally used two-dimensional gel electrophoresis (2DE) for separation and characterisation of complex protein mixtures. Among the difficulties associated with this approach is the solubilisation of protein mixtures for isoelectric focusing (IEF)...

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Detalles Bibliográficos
Autores principales:	Khoudoli, Guennadi A, Porter, Iain M, Blow, J Julian, Swedlow, Jason R
Formato:	Texto
Lenguaje:	English
Publicado:	BioMed Central 2004
Materias:	Methodology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC517948/ https://www.ncbi.nlm.nih.gov/pubmed/15357868 http://dx.doi.org/10.1186/1477-5956-2-6

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