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Highly Efficient CRISPR/Cas9-Mediated Homologous Recombination Promotes the Rapid Generation of Bacterial Artificial Chromosomes of Pseudorabies Virus

Bacterial artificial chromosomes (BACs) are powerful tools for the manipulation of the large genomes of DNA viruses, such as herpesviruses. However, the methods currently used to construct the recombinant viruses, an important intermediate link in the generation of BACs, involve the laborious proces...

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Detalles Bibliográficos
Autores principales:	Guo, Jin-Chao, Tang, Yan-Dong, Zhao, Kuan, Wang, Tong-Yun, Liu, Ji-Ting, Gao, Jia-Cong, Chang, Xiao-Bo, Cui, Hong-Yu, Tian, Zhi-Jun, Cai, Xue-Hui, An, Tong-Qing
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2016
Materias:	Microbiology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5179515/ https://www.ncbi.nlm.nih.gov/pubmed/28066407 http://dx.doi.org/10.3389/fmicb.2016.02110

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